A5044306653- MicroRNA in disease regulation
- Circular RNAs in diseases
- Multiple Myeloma Research and Treatments
- Cancer-related molecular mechanisms research
- Antibiotic Resistance in Bacteria
- Rheumatoid Arthritis Research and Therapies
- Escherichia coli research studies
- Cancer therapeutics and mechanisms
- Protein Degradation and Inhibitors
- Enterobacteriaceae and Cronobacter Research
- Histone Deacetylase Inhibitors Research
- Bone and Joint Diseases
- Cancer Treatment and Pharmacology
- Chemotherapy-induced cardiotoxicity and mitigation
- Clay minerals and soil interactions
- Retinopathy of Prematurity Studies
- Toxoplasma gondii Research Studies
- Antibiotics Pharmacokinetics and Efficacy
- Cytokine Signaling Pathways and Interactions
- Nanoparticle-Based Drug Delivery
- Pharmaceutical and Antibiotic Environmental Impacts
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Epigenetics and DNA Methylation
- Neonatal Respiratory Health Research
- Corneal surgery and disorders
University of Florida
2020-2024
Beni-Suef University
2022-2023
Center for Rheumatology
2023
National Student Clearinghouse Research Center
2021
Florida College
2021
Children Cancer Hospital
2015-2020
Kafrelsheikh University
2018
Al-Azhar University
2017
Cairo University
2011-2013
Human Cytomegalovirus (HCMV) is an endemic herpes virus that re-emerges in cancer patients enhancing oncogenic potential. Recent studies have shown HCMV infection associated with certain types of morbidity such as glioblastoma. Although has been detected breast tissues, its role, if any, the etiology specific forms not investigated. In present study we investigated presence inflammatory (IBC), a rapidly progressing form characterized by molecular signature. We screened for anti-CMV IgG...
MicroRNAs (miRNAs) have been repeatedly shown to play important roles in liver pathologies, including hepatitis, cirrhosis, and cancer. Egypt has the highest hepatitis C virus (HCV) infection rate worldwide, predominantly involving genotype-4.In this study, we attempted characterize miRNA profile of poorly studied genotype 4 HCV chronically infected Egyptian patients obtain a better understanding disease its complications help design management protocols.We analyzed expression levels...
Understanding pediatric brain tumor biology is essential to help on disease stratification, and find novel markers for early diagnosis. MicroRNA (miRNA) expression has been linked clinical outcomes biology. Here, we aimed detect the of different miRNAs in subtypes discover biomarkers detection develop therapies. Expression 82 was detected 120 tumors from fixed-formalin paraffin-embedded tissues, low-grade glioma, high-grade ependymoma, medulloblastoma, using quantitative real-time PCR....
Hepatocellular carcinoma (HCC) is a lethal malignancy with poor prognosis and limited treatment options. Transarterial chemoembolization (TACE) using chemotherapy agents—doxorubicin cisplatin—is an accepted option for locally advanced hepatocellular carcinoma. In the current study, we analyzed expression pattern of selected panel 94 miRNAs in archival samples that were collected prior to from 15 Egyptian patients diagnosed hepatocelleular We observed overall increase miRNA HCC compared...
Background Thiazide diuretics are the second most frequently prescribed class of antihypertensives, but up to 50% patients with hypertension have minimal antihypertensive response thiazides. We explored circulating microRNAs (miRNAs) in search predictive biomarkers thiazide response. Methods and Results profiled 754 miRNAs baseline plasma samples 36 hypertensive European American adults treated hydrochlorothiazide, categorized into responders (n=18) nonresponders on basis diastolic blood...
Infection with multiple drug resistant (MDR) Escherichia coli poses a life threat to immunocompromised pediatric cancer patients. Our aim is genotypically characterize the plasmids harbored in MDR E. isolates recovered from bacteremic patients of Children's Cancer Hospital Egypt 57357 (CCHE 57357). In this study, 21 carbapenem-resistant (CRE) were selected that exhibit Quinolones and Aminoglycosides resistance. Plasmid shot-gun sequencing was performed using Illumina next- generation...
Mutations in the mitochondrial genome (mtDNA) are associated with different types of cancer, specifically colorectal cancer (CRC). However, few studies have been performed on precancerous lesions, such as ulcerative colitis (UC) lesions and adenomatous polyps (AP). The aim this study was to identify mtDNA mutations cancerous Egyptian patients. An analysis found six regions (ND1, ND5, COI, tRNAser, D-loop 1, 2) 80 patients (40 CRC, 20 UC, AP) using polymerase chain reaction-single-strand...
Cytochrome P450 (CYP) drug metabolizing enzymes are responsible for the metabolism of over 70% currently used medications with CYP3A family being most important CYP in liver. Large inter-person variability expression/activity CYP3As greatly affects exposure and treatment outcomes, yet cause such remains elusive. Micro-RNAs (miRNAs) small noncoding RNAs that negatively regulate gene expression involved diverse cellular processes including xenobiotics therapeutic outcomes. Target prediction...
Abstract As a treatment for relapsed or refractory multiple myeloma (MM), carfilzomib has been associated with significant risk of cardiovascular adverse events (CVAE). The goals our study were to evaluate the metabolomic profile MM patients identify those at high prior and explore mechanisms carfilzomib‐CVAE inform potential strategies protect from this cardiotoxicity. Global profiling was performed on baseline post‐baseline plasma samples 60 treated carfilzomib‐based therapy, including 31...
Multiple myeloma (MM) is the second most frequent hematologic cancer in United States. Carfilzomib (CFZ), an irreversible proteasome inhibitor being used to treat relapsed and refractory MM, has been associated with cardiotoxicity, including heart failure. We hypothesized that a multi-omics approach integrating data from different omics would provide insights into mechanisms of CFZ-related cardiovascular adverse events (CVAEs). Plasma samples were collected 13 MM patients treated CFZ...
Proteasome inhibitor Carfilzomib (CFZ) is effective in treating patients with refractory or relapsed multiple myeloma (MM) but has been associated cardiovascular adverse events (CVAE) such as hypertension, cardiomyopathy, and heart failure. This study aimed to investigate the contribution of germline genetic variants protein-coding genes CFZ-CVAE among MM using whole-exome sequencing (WES) analysis.Exome-wide single-variant association analysis, gene-based rare variant analyses were...
To identify CYP1B1 gene mutations and evaluate their possible role as a prognostic factor for success rates in the surgical management of Egyptian congenital glaucoma patients.Totally 42 eyes 29 primary patients were operated on with combined trabeculotomy/trabeculectomy mitomycin-C followed up at 1d, 1wk, 1, 6 12mo postoperatively. Genomic DNA was extracted from peripheral blood leukocytes. Coding regions amplified using 13 pairs primers, screened single-strand conformation polymorphism by...
Aim: The aim of this study was to evaluate the diagnostic and prognostic performance miRNA-29a miRNA-100 in pediatric acute myeloid leukemia (AML). Patients Methods: In all, 73 children with diagnosed AML (based on standard morphologic, cytochemical, cytogenetic, immunologic, molecular workup, French-American British classification) admitted Children’s Cancer Hospital Egypt (CCHE-57357), 9 healthy age-matched sex-matched controls were recruited for a case-control study. Gene expression...
The epidemiology, Pathogencity and the possible ways of treatment Klebsiella pneumoniae (K. pneumoniae) infection as a respiratory pathogen in broiler chicken farms different places Gharbia Goernorate were investigated during 2014/2017.For this purpose 150 samples collected from lung, trachea, , liver intestine diseased, 1-5-week-old commercial chickens suffered disorders, diarrhea, growth retardation mortality with pneumonia, pericarditis, airsaculitis enlarged liver. Bacteriological...
Abstract Introduction: Human hepatocellular carcinoma (HCC) is consider one of the most common and lethal tumors worldwide, HCC currently fifth solid tumor worldwide fourth important cause cancer-related death. Eighty percent new cases found in developing countries as Egypt, but incidence increasing economically developed regions, including Japan, Western Europe, United States. It has shown that expression level different miRNAs was correlated with cellular liver processes such inflammation,...
Background and objectives: Although hepatoblastoma (HB) is a rare childhood tumor constitutes only 0.9% of all pediatric cancers, there was an obvious improvement in risk stratification prognosis over the last two decades. This study aimed to assess outcome HB patients treated our center investigate impact different prognostic factors on survival these patients. Patients methods: retrospective included newly diagnosed with presented Children Cancer Hospital Egypt (CCHE 57357), from July 2007...
This study investigates the association between circulating microRNA (miRNA) expression and cardiovascular adverse events (CVAE) in multiple myeloma (MM) patients treated with a carfilzomib (CFZ)-based regimen. A cohort of 60 MM from Prospective Observation Cardiac Safety Proteasome Inhibitor (PROTECT) was analyzed. Among these, 31 (51.6%) developed CVAE post-CFZ treatment. The Taqman OpenArray Human panels were used for miRNA profiling. We identified 13 differentially expressed miRNAs at...
Background: Inflammatory rheumatoid arthritis (RA) worsens over time.Severe symptoms can be avoided with an early diagnosis.RA increases CRP-albumin ratio (CAR) as acute-phase reactants.Objective: To validate the CAR inflammatory impact in RA and how it correlates disease activity indices musculoskeletal ultrasonography.Methods: Seventy-five patients were involved this cross-sectional research.50 normal subjects included a control group.All participants tested for CRP, albumin, CAR.Disease...
ABSTRACT The nucleos(t)ide analogs require phosphorylation to the pharmacologically active anabolites in cells. We investigated hypothesis that single-nucleotide polymorphisms (SNPs) genes encode transporters and phosphodiesterase (PDE) enzymes involved tenofovir (TFV), disoproxil fumarate (TDF), lamivudine (3TC) disposition will be associated with concentrations of their phosphate virologic response. Individuals human immunodeficiency virus (HIV) hepatitis B (HBV) coinfection receiving...
In Garb El-Mawhob area, northwest of El-Dakhla Oasis, New Valley governorate, Egypt, nine transects (≈ 5km away between two consecutive ones) containing 17 soil sites were designated to evaluate the available potassium (K)and its relation with some properties.Surface (0-30) and subsurface (30-60) samples collected from each profile, air-dried, ground, sieved then, kept for physical chemical analyses.The obtained results indicated that about 56, 65 41% total contained sand, silt clay...