A5041757700- Advanced NMR Techniques and Applications
- Protein Structure and Dynamics
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Enzyme Structure and Function
- Solid-state spectroscopy and crystallography
- DNA and Nucleic Acid Chemistry
- Photochromic and Fluorescence Chemistry
- Organophosphorus compounds synthesis
- Food composition and properties
- Biofuel production and bioconversion
- Muon and positron interactions and applications
- NMR spectroscopy and applications
- Analytical Chemistry and Chromatography
- Asymmetric Hydrogenation and Catalysis
- Photoreceptor and optogenetics research
- Epilepsy research and treatment
- Atomic and Subatomic Physics Research
- Polysaccharides and Plant Cell Walls
- Alcoholism and Thiamine Deficiency
- Historical Art and Culture Studies
- Infectious Encephalopathies and Encephalitis
- Advanced biosensing and bioanalysis techniques
- RNA modifications and cancer
- Cultural Heritage Management and Preservation
Goethe University Frankfurt
2017-2023
Budapest University of Technology and Economics
2012
NMR sensitivity-enhancement methods involving hyperpolarized water could be of importance for solution-state biophysical investigations. Hyperpolarized (HyperW) can enhance the 1 H signals exchangeable sites by orders magnitude over their thermal counterparts, while providing insight into chemical exchange and solvent accessibility at a site-resolved level. As HyperW’s enhancements are achieved exploiting fast exchanges associated with minimal interscan delays, possibilities rapid monitoring...
Both experimental and theoretical structure determinations of RNAs have remained challenging due to the intrinsic dynamics RNAs. We report here an integrated nuclear magnetic resonance/molecular (NMR/MD) determination approach describe dynamic CUUG tetraloop. show that tetraloop undergoes substantial dynamics, leading averaging data. These are particularly linked temperature-dependent presence a hydrogen bond within Interpreting NMR data by single represents low-temperature well but fails...
The microwave-assisted double Kabachnik–Fields (phospha-Mannich) reaction of cyclohexylamine, two equivalents paraformaldehyde and > P(O)H species, such as dialkyl phosphites, ethyl phenyl-H-phosphinate diphenylphosphine oxide affords the title compounds in good yields. bis(diphenylphosphinoxido) derivative was converted to corresponding bidentate chelate platinum complex steps, whose stereostructure elucidated by quantum chemical calculations. crystal structure studied X-ray analysis....
Photolabile protecting groups are widely used to trigger oligonucleotide activity. The ON/OFF-amplitude is a critical parameter. An experimental setup has been developed identify group derivatives with superior caging properties. Bulky rests attached the cage moiety via Cu-catalyzed azide-alkyne cycloaddition post-synthetically on DNA. Interestingly, decrease in melting temperature upon introducing o-nitrobenzyl-caged (NPBY-) and diethylaminocoumarin-cages (DEACM-) DNA duplexes reaches...
A simple system has been constructed for the quantitative determination of barley β-glucans. Measurements were made by flow-injection-analysis (FIA) technique using fluorescent dye Calcofluor as a specific reagent (1·3) (1·4)–B—D—linkages. In this paper single-line is described involving only one pump samples β-glucan being directly injected into stream. The reaction results in an increased intensity fluorescence detected flow-through-cell fluorimeter and evaluated peak heights or areas...
The C40A/C82A double mutant of barstar has been shown to undergo cold denaturation above the water freezing point. By rapidly applying radio-frequency power lossy aqueous samples, refolding from its cold-denatured state can be followed by real-time NMR spectroscopy. Since temperature-induced unfolding and is reversible for this mutant, multiple cycling utilized obtain 2D data. Barstar contains two proline residues that adopt a mix cis trans conformations in low-temperature-unfolded state,...
We shall overcome: As a result of efforts to overcome the sensitivity challenge liquid-state NMR spectroscopy, thousand-fold signal enhancement was achieved by dynamic nuclear polarization (DNP) for 13 C signals at high magnetic field (3.4 T) and room temperature, thereby exceeding predicted limitations high-field in situ DNP.
Grenzen durchbrechen: Eine 1000-fache Verstärkung von 13C-Signalen in der Flüssigphasen-NMR-Spektroskopie wurde durch dynamische Kernpolarisation (DNP) bei hohem Magnetfeld und Raumtemperatur erzielt. Die früher vorhergesagte Obergrenze für Hochfeld-DNP flüssiger Phase damit deutlich übertroffen. “Die Schwäche Kernspinwechselwirkungen, die bislang als Vorteil dargestellt wurde, führt allerdings zu gravierenden Schwierigkeiten Detektion. große Zahl Spins ist notwendig, um schwachen Signale...
Abstract The C40A/C82A double mutant of barstar has been shown to undergo cold denaturation above the water freezing point. By rapidly applying radio‐frequency power lossy aqueous samples, refolding from its cold‐denatured state can be followed by real‐time NMR spectroscopy. Since temperature‐induced unfolding and is reversible for this mutant, multiple cycling utilized obtain 2D data. Barstar contains two proline residues that adopt a mix cis trans conformations in low‐temperature‐unfolded...
Abstract. The review describes the application of NMR spectroscopy to study kinetics folding, refolding and aggregation proteins, RNA DNA. Time-resolved experiments can be conducted in a reversible or an irreversible manner. In particular folding pose large requirements on (i) signal-to-noise due time limitations (ii) synchronizing steps. Thus, this contribution discusses methods for increases including dynamic nuclear polarization, hyperpolarization photo-CIDNP time-resolved studies....