A5083196300- Plant and fungal interactions
- Fungal Biology and Applications
- Plant Pathogens and Fungal Diseases
- Botanical Research and Chemistry
- Mycorrhizal Fungi and Plant Interactions
- Legume Nitrogen Fixing Symbiosis
- Plant-Microbe Interactions and Immunity
- Law and Political Science
- Photochromic and Fluorescence Chemistry
- Viral Infections and Immunology Research
- Acute Myeloid Leukemia Research
- Bacteriophages and microbial interactions
- Plant Pathogenic Bacteria Studies
- Nematode management and characterization studies
- Photoreceptor and optogenetics research
- Protein Degradation and Inhibitors
- Viral Infectious Diseases and Gene Expression in Insects
- Plant Pathogens and Resistance
- Virus-based gene therapy research
- Research on scale insects
- Ubiquitin and proteasome pathways
- Plant Taxonomy and Phylogenetics
- Yeasts and Rust Fungi Studies
- Metalloenzymes and iron-sulfur proteins
- Genomics and Chromatin Dynamics
Julius Kühn-Institut
2018-2024
University Hospital Bonn
2023
Goethe University Frankfurt
2008-2020
University of Bonn
2019
Leibniz Institute of Vegetable and Ornamental Crops
2016-2018
Massey University
2011-2018
Georg Speyer Haus
2007-2010
University of Tübingen
2008
Regulated synthesis of reactive oxygen species (ROS) by membrane-bound fungal NADPH oxidases (Nox) plays a key role in morphogenesis, growth, and development. Generation the plant symbiotic fungus, Epichloë festucae , requires functional assembly multisubunit complex composed NoxA, regulatory component, NoxR, small GTPase RacA. However, mechanism for activation this at plasma membrane is unknown. We found yeast two-hybrid coimmunoprecipitation assays that E. NoxR interacts with homologs...
Summary Epichloë festucae forms a mutualistic symbiotic association with Lolium perenne . This biotrophic fungus systemically colonizes the intercellular spaces of aerial tissues to form an endophytic hyphal network. E. also grows as epiphyte, but mechanism for leaf surface colonization is not known. Here we identify appressorium‐like structure, which call expressorium that allows hyphae penetrate cuticle from inside establish epiphytic net on leaf. We used combination scanning electron,...
Abstract In this study fungal strains were investigated, which had been isolated from eggs of the cereal cyst nematode Heterodera filipjevi , and roots Microthlaspi perfoliatum ( Brassicaceae ). The morphology, interaction with nematodes plants phylogenetic relationships these originating a broad geographic range covering Western Europe to Asia Minor studied. Phylogenetic analyses using five genomic loci including ITSrDNA, LSUrDNA, SSUrDNA, rpb2 tef1-α carried out. found represent distinct...
Epichloë festucae is a mutualistic symbiont that systemically colonizes the intercellular spaces of Lolium perenne leaves to form highly structured and interconnected hyphal network. In an Agrobacterium tumefaciens T-DNA forward genetic screen, we identified mutant TM1066 had severe host interaction phenotype, causing stunting premature senescence host. Molecular analysis revealed mutation responsible for this phenotype was in cell-wall integrity (CWI) mitogen-activated protein kinase...
The recent worldwide discovery of plant growth-promoting (PGP) Kosakonia radicincitans in a large variety crop plants suggests that this species confers significant influence on plants, both terms yield increase and product quality improvement. We provide comparative genome analysis which helps to unravel the genetic basis for K. radicincitans' motility, competitiveness capacities. discovered carries multiple copies complex gene clusters, among them two flagellar systems three type VI...
With a new photolabile protecting group – exclusively cleavable by two-photon-excitation complex light scenarios for three-dimensional uncaging are possible.
Epichloë festucae is an endophyte of the agriculturally important perennial ryegrass. This species systemically colonises aerial tissues this host where its growth tightly regulated thereby maintaining a mutualistic symbiotic interaction. Recent studies have suggested that small secreted proteins, termed effectors, play vital role in suppression defence responses. To date only few effectors with roles interactions been described. Here we make use fully assembled E. genome and EffectorP to...
Summary Cell–cell fusion in fungi is required for colony formation, nutrient transfer and signal transduction. Disruption of genes hyphal Epichloë festucae , a mutualistic symbiont Lolium grasses, severely disrupts the host interaction phenotype. They examined whether symB symC E. homologs Podospora anserina self‐signaling IDC2 IDC3 are symbiosis. Deletion mutants these were defective cell fusion, formed intra‐hyphal hyphae, had enhanced conidiation. SymB‐GFP SymC‐mRFP1 localize to plasma...
Summary In both Sordaria macrospora and Neurospora crassa , components of the conserved STRIPAK (striatin‐interacting phosphatase kinase) complex regulate cell–cell fusion, hyphal network development fruiting body formation. Interestingly, a number Epichloë festucae genes that are required for such as noxA noxR proA mpkA mkkA also establishment mutualistic symbiotic interaction with Lolium perenne . To determine whether MobC, homologue component MOB3 in S. N. is E. fusion symbiosis, mobC...
Natural hosts for the fungal endophyte Epichloë festucae include Festuca rubra (fine fescue) and trachyphylla (hard fescue). Some strains also form stable associations with Lolium perenne (perennial ryegrass). L. is a suitable host to study endophyte-grass interactions, such as endophytic growth within plant epiphyllous on surface. Here we provide detailed protocol based work by, artificial inoculation of E. into perenne, newly developed staining visualization techniques observing hyphae...
Photolabile protecting groups are widely used to trigger oligonucleotide activity. The ON/OFF-amplitude is a critical parameter. An experimental setup has been developed identify group derivatives with superior caging properties. Bulky rests attached the cage moiety via Cu-catalyzed azide-alkyne cycloaddition post-synthetically on DNA. Interestingly, decrease in melting temperature upon introducing o-nitrobenzyl-caged (NPBY-) and diethylaminocoumarin-cages (DEACM-) DNA duplexes reaches...
// Yvonne Becker 1 , * Sarah Förster Gerrit H. Gielen 2 Ian Loke 3 Morten Thaysen-Andersen Christine Laurini Kristin Wehrand Torsten Pietsch and Simone Diestel Institute of Nutrition Food Sciences, Department Human Metabolomics, University Bonn, Bonn 53115, Germany Neuropathology, Medical Center, 53127, Molecular Macquarie University, Sydney, NSW 2109, Australia These authors contributed equally to this work Correspondence to: Diestel, email: s.diestel@uni-bonn.de Keywords: cancer...
We developed three bathochromic, green-light activatable, photolabile protecting groups based on a nitrodibenzofuran (NDBF) core with D-π-A push-pull structures. Variation of donor substituents (D) at the favored ring position enabled us to observe their impact photolysis quantum yields. Comparing our new azetidinyl-NDBF (Az-NDBF) group earlier published DMA-NDBF, we obtained insight into its excitation-specific photochemistry. While "two-photon-only" cage DMA-NDBF was inert against...
Abstract Tilletia caries and T. laevis , which are the causal agents of common bunt, as well controversa, causes dwarf bunt wheat, threaten especially organic wheat farming. The three closely related fungal species differ in their teliospore morphology partially physiology infection biology. gene content intraspecies variation these genetic basis separation is unknown. We sequenced genome four five controversa two extended this dataset with publicly available ones. genomes displayed...
The conformational dynamics of proteins plays a key role in their complex physiological functions. Fluorescence resonance energy transfer (FRET) is particular powerful tool for studying protein dynamics, but requires efficient site-specific labeling with fluorescent reporter probes. We have employed different tris-NTA/fluorophore conjugates, which bind histidine-tagged high affinity, incorporation FRET acceptors into proteins, were covalently labeled donor fluorophore. demonstrate versatile...
Here we describe a new, haploid and stroma forming species within the genus Epichloë, as Epichloë scottii sp. nov. The fungus was isolated from Melica uniflora growing in Bad Harzburg, Germany. Phylogenetic reconstruction using combined dataset of tubB tefA genes strongly support that E. is distinct so far unknown ancestor hybrid disjuncta. A distribution analysis showed high infection rate close vicinity initial sampling site only two more spots with low rates. Genetic variations key...
Neuropilin-2 (NRP2) is a cell surface receptor that plays key roles in lymphangiogenesis, but also pathophysiological conditions such as cancer and inflammation. NRP2 targeting by efzofitimod, novel immunomodulatory molecule, currently being tested for the treatment of pulmonary sarcoidosis. To date, no anti-NRP2 antibodies are available companion diagnostics. Here we describe development characterization antibody. Using variety research techniques, is, enzyme-linked immunoassay, Western...
Fungi from the genus Epichloë form systemic endobiotic infections of cool season grasses, producing a range host-protective natural products in return for access to nutrients. These are asymptomatic during vegetative host growth, with associations between asexual spp. and their hosts considered mutualistic. However, sexual cycle involves virulent characterized by envelopment sterilization developing inflorescence dense sheath mycelia known as stroma. Microscopic analysis stromata revealed...