Chemical exploration for two isolates of the recently described ascomycete species Polyphilus sieberi, derived from eggs plant parasitic nematode Heterodera filipjevi, afforded identification many compounds that belong to various metabolite families: previously undescribed chlorinated cyclotetrapeptides, omnipolyphilins A (1) and B (2), one new pyranonaphthoquinone, ventiloquinone P (3), a 6,6′-binaphto-α-pyranone dimer, talaroderxine D (4) in addition nine known metabolites (5–13) were...

Abstract In this study fungal strains were investigated, which had been isolated from eggs of the cereal cyst nematode Heterodera filipjevi , and roots Microthlaspi perfoliatum ( Brassicaceae ). The morphology, interaction with nematodes plants phylogenetic relationships these originating a broad geographic range covering Western Europe to Asia Minor studied. Phylogenetic analyses using five genomic loci including ITSrDNA, LSUrDNA, SSUrDNA, rpb2 tef1-α carried out. found represent distinct...

Cyst nematodes are globally important pathogens in agriculture. Their sedentary lifestyle and long-term association with the roots of host plants render cyst especially good targets for attack by parasitic fungi. In this context fungi were specifically isolated from nematode eggs cereal Heterodera filipjevi. Here, Ijuhya vitellina (Ascomycota, Hypocreales, Bionectriaceae), encountered wheat fields Turkey, is newly described on basis phylogenetic analyses, morphological characters life-style...

Fungal root endophytes, including the common group of dark septate endophytes (DSEs), represent different taxonomic groups and potentially diverse life strategies. In this study, we investigated two unidentified helotialean lineages found previously in a study DSE fungi semiarid grasslands, from several other sites, collected recently pezizalean truffle ascoma eggs cereal cyst nematode Heterodera filipjevi. The positions phylogenetic relationships 21 isolates with hosts geographic origins...

Motor pools comprise a heterogeneous population of motor neurons that innervate distinct intramuscular targets. While the organization into has been well described, time course and mechanism pool diversification functionally classes remains unclear. γ-Motor (γ-MNs) α-motor (α-MNs) differ in size, molecular identity, synaptic input peripheral target. α-MNs extrafusal skeletal muscle fibers to mediate contraction, γ-MNs intrafusal spindle, regulate sensitivity spindle response stretch. In this...

The new cyclic lipodepsipeptide ophiotine (1), two arthrichitin derivatives named arthrichitins B (4) and C (5), a xanthocillin-like alkaloid, xanthomide Z (2), the previously described (3) were isolated from liquid culture broth of nematode-associated fungus with affinities to genus Ophiosphaerella. structural elucidation determination absolute configuration molecules accomplished using combination spectroscopic chemical techniques, including 1D 2D NMR, HRMS, Marfey's analysis. Opiotine (1)...

Abstract Finding beneficial fungi antagonistic toward nematodes is important for developing more sustainable agricultural practices. We isolated abundant from cysts of the beet cyst nematode Heterodera schachtii and tested their interaction with sugar beet. By molecular screening fungal communities colonizing five field populations H. using DGGE Sanger sequencing, three strains identified as Exophiala sp., Pochonia chlamydosporia Pyrenochaeta sp. were frequently found in infected then...

Root nodules of legume plants are primarily inhabited by rhizobial nitrogen-fixing bacteria. Here, we propose two new Rhizobiales species isolated from root common sainfoin (Onobrychis viciifolia), as shown core-gene phylogeny, overall genome relatedness indices, and pan-genome analysis. Mesorhizobium onobrychidis sp. nov. actively induces achieves atmospheric nitrogen carbon dioxide fixation. This appears to be depleted in motility genes is enriched for direct effects on plant growth...

In the course of our survey to study metabolic potential two species a new helotialean genus Polyphilus, namely P. frankenii and sieberi, their crude extracts were obtained using different cultivation techniques, which led isolation characterization naphtho-α-pyranone derivatives recognized as monomer (1) its 6,6'-homodimer (2) together with known diketopiperazine congeners, outovirin B (3) (3S,6S)-3,6-dibenzylpiperazine-2,5-dione (4). The structures isolated compounds determined based on...

Monocillium gamsii sp. nov. (Ascomycota, Hypocreales, Niessliaceae) isolated from eggs of the cereal cyst nematode Heterodera filipjevi is described and illustrated based on morphological molecular phylogenetic evidence. The new taxon discovered in wheat fields Turkey destructively parasitises eggs. infected were readily colonised by fungus, which produced microsclerotia. fungus could be grown artificial media parasitism M. towards H. was reproducible vitro. Hyphae penetrating entirely...

Fungal associations with nematodes have attracted scientific attention because of the need to develop new biocontrol agents. In this context, Ijuhya vitellina, an antagonistic fungus previously isolated from plant parasitic cyst nematode Heterodera filipjevi, was selected carry out in-depth metabolomic study for its active metabolites. Herein, three nonapeptide peptaibols leucinostatin based sequences were and identified by 1, 2D NMR, HR-ESI-MS-MS. The absolute configuration assigned on...

Abstract Fungal root endophytes, including the common form group of dark septate endophytes (DSEs), represent different taxonomic groups with potentially diverse life strategies. During surveys DSE communities and nematode cysts colonizing fungi, isolates representing Laburnicola ( Didymosphaeriaceae , Pleosporales ) lineages were discovered. Here we carried out a comprehensive study phylogenetic relationships taxonomy fungi collected from plant roots in Hungary, Mongolia, Kazakhstan eggs...

As a part of our ongoing research on nematode-associated fungi, we are aiming to identify sec-ondary metabolites that have unique structures and/or biological activities. The cultivation Laburnicola nematophila, isolated from cysts the plant parasitic nematode Heterodera filipjevi affored three dactylofungin derivatives (1–3) and tetralone congeners (4–6). Dactylofungin C (1), laburnicolin (4) laburnicolenone (5) revealed be previously undescribed natural products. chemical compounds were...

Nematode-associated fungi revealed the potential to produce a broad spectrum of chemical scaffolds. In this study, mycelial extract Laburnicola nematophila, fungal strain derived from cereal cyst nematode Heterodera filipjevi, was chemically explored and afforded six unprecedentedly reported acylic N-acetyl oligopeptides, laburnicotides A–F (1–6). Structure elucidation isolated compounds established based on comprehensive 1D 2D NMR spectroscopic analyses together with acquired HR-ESI-MS...

A new disease was observed on chamomile plants (Matricaria recutita) in various commercially cultivated fields 2016 and 2017 Germany. From symptomatic stems leaves, the fungal species described here isolated repeatedly. Kochʼs postulates using planta experiments were fulfilled by inoculation of proving fungus to be causal agent disease. Morphological studies phylogenetic analyses internal transcribed spacer, large subunit, translation elongation factor-1α sequences suggested that represents...

Root-knot nematodes (RKNs) are one of the most important plant-parasitic cereal crops in sub-Saharan Africa. This study was designed to evaluate rotation effects different cultivars sainfoin (Esparsette, Perly, Taja and Visnovsky), soybean (DM-5953-RSF) alfalfa (BAR 7) with maize (P-2432-R), on a Meloidogyne enterolobii population, compared monoculture maize. The results showed that (Perly Esparsette) had significantly ( P ≤ 0.05) lower numbers M. eggs second stage juveniles (J2) first...

Here we describe a new, haploid and stroma forming species within the genus Epichloë, as Epichloë scottii sp. nov. The fungus was isolated from Melica uniflora growing in Bad Harzburg, Germany. Phylogenetic reconstruction using combined dataset of tubB tefA genes strongly support that E. is distinct so far unknown ancestor hybrid disjuncta. A distribution analysis showed high infection rate close vicinity initial sampling site only two more spots with low rates. Genetic variations key...

Meloidoderita salinasp. n. is described and illustrated from the halophytic plant Atriplex portulacoides L. (sea purslane) growing in a micro-tidal salt marsh Mont-Saint-Michel Bay France. This new species first member of Poghossian, 1966 collected saline environment, characterized by following features: sedentary mature females having small swollen body with clear posterior protuberance; slightly dorsally curved stylet, 19.9 µm long, posteriorly sloping knobs; neck region irregular shape...

Plant-parasitic nematodes are one of the main biotic factors limiting agricultural production worldwide, with root-knot (Meloidogyne spp.) being most damaging group. This study was conducted to evaluate efficacy soil microbiomes, associated various subtropical fruit trees, on management a Meloidogyne enterolobii population. Of 14 microbiomes tested for nematode suppression, 9 samples in first experiment and 10 repeat had significantly (p ≤ 0.05) lower numbers eggs J2 compared untreated...

The downy mildew species parasitic to Mentheae are of particular interest, as this tribe Lamiaceae contains a variety important medicinal plants and culinary herbs. Over the past two decades, pathogens, Peronospora belbahrii Pe. salviae-officinalis have spread globally, impacting basil common sage production, respectively. In original circumscription belbahrii, coleus (Plectranthus scutellarioides) was ascribed in broader sense, but subtle differences morphological molecular phylogenetic...