A5055599966- Chemical Synthesis and Analysis
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- RNA and protein synthesis mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Antimicrobial Peptides and Activities
- Biochemical and Structural Characterization
- Microfluidic and Capillary Electrophoresis Applications
- Click Chemistry and Applications
- Microbial Natural Products and Biosynthesis
- Advanced biosensing and bioanalysis techniques
- Various Chemistry Research Topics
- Biotin and Related Studies
- Tuberculosis Research and Epidemiology
- Cyclopropane Reaction Mechanisms
- Boron and Carbon Nanomaterials Research
- Nanocluster Synthesis and Applications
- Molecular Junctions and Nanostructures
- Organoboron and organosilicon chemistry
- Boron Compounds in Chemistry
- Genetics, Bioinformatics, and Biomedical Research
Massachusetts Institute of Technology
2019-2023
University of California, Los Angeles
2016
Fully synthetic whole proteins in reach Solid-phase peptide synthesis of homogeneous peptides longer than about 50 amino acids has been a long-standing challenge because inefficient coupling and side reactions. Hartrampf et al. used an automated chemistry platform to optimize fast-flow were able produce fully single-domain (see the Perspective by Proulx). The targets included proinsulin enzymes such as barnase version HIV-1 protease containing multiple noncanonical acids. Refolded nearly...
Few chemical methods exist for the covalent conjugation of two proteins. We report preparation site-specific protein-protein conjugates that arise from sequential cross-coupling cysteine residues on different The method involves synthesis stable palladium-protein oxidative addition complexes (Pd-protein OACs), a process converts nucleophilic into an electrophilic S-aryl-Pd-X unit by taking advantage intramolecular strategy. This is demonstrated proteins up to 83 kDa in size and can be...
The impermeable outer membrane of Pseudomonas aeruginosa is bypassed by antibacterial proteins known as S-type pyocins. Because their properties, pyocins are investigated a potential new class antimicrobials against infections. Their production and modification, however, remain challenging. To address this limitation, we employed automated fast-flow peptide synthesis for the rapid pyocin S2 import domain. N-terminal domain sequence (PyS2NTD) was synthesized in under 10 h purified to yield...
Pseudomonas aeruginosa is a major bacterial pathogen associated with rising prevalence of antibiotic resistance. We evaluated the resistance mechanisms P. against POL7080, species-specific, first-in-class in clinical trials that targets lipopolysaccharide transport protein LptD. isolated series POL7080-resistant strains mutations two-component sensor gene pmrB .
<p>Ribosomes produce most proteins of living cells in seconds. Here we report highly efficient chemistry matched with an automated fast-flow instrument for the direct manufacturing peptide chains up to 164 amino acids over 328 consecutive reactions. The machine is rapid - chain elongation complete hours. We demonstrate utility this approach by chemical synthesis nine different protein that represent enzymes, structural units, and regulatory factors. After purification folding,...
Ribosomes produce most proteins of living cells in seconds. Here we report highly efficient chemistry matched with an automated fast-flow instrument for the direct manufacturing peptide chains up to 164 amino acids over 328 consecutive reactions. The machine is rapid - chain elongation complete hours. We demonstrate utility this approach by chemical synthesis nine different protein that represent enzymes, structural units, and regulatory factors. After purification folding, synthetic...
Abstract The impermeable outer membrane of Pseudomonas aeruginosa is bypassed by antibacterial proteins known as S-type pyocins. Because their properties, pyocins are investigated a potential new class antimicrobials against infections. Their production and modification, however, remains challenging. To address this limitation, we employed automated fast-flow peptide synthesis (AFPS) for the rapid pyocin S2 import domain. N-terminal domain sequence (PyS2 NTD ) was synthesized in under 10...
This work describes select narratives pertaining to undergraduate teaching and mentorship at UCLA Chemistry Biochemistry by Alex Spokoyny his junior colleagues. Specifically, we discuss how individual researchers contributed jump-started multiple research themes since the conception of our laboratory. also several recent innovations in inorganic general chemistry courses taught with a focus nurturing appreciation for creative process sciences including use social media platforms.
Pseudomonas aeruginosa is a major bacterial pathogen for which there rising antibiotic resistance. We evaluated the resistance mechanisms of P. against POL7080, species-specific, first-in-class in phase 3 clinical trials targeting lipopolysaccharide transport protein LptD. found mutations two-component regulator pmrB . Genome-wide transcriptomics and confocal microscopy studies together suggest that POL7080 vulnerable to same described previously polymyxins, including colistin, involve lipid...