A5031434363- RNA modifications and cancer
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- Cancer-related molecular mechanisms research
- Genetics and Neurodevelopmental Disorders
- Peptidase Inhibition and Analysis
- Cancer Cells and Metastasis
- Chromatin Remodeling and Cancer
- MicroRNA in disease regulation
- Mechanisms of cancer metastasis
- Cancer-related gene regulation
- Advanced biosensing and bioanalysis techniques
- S100 Proteins and Annexins
- Genomics and Chromatin Dynamics
- Genomics and Phylogenetic Studies
- Neurogenesis and neuroplasticity mechanisms
- Renal cell carcinoma treatment
- Aging and Gerontology Research
- Barrier Structure and Function Studies
- Muscle Physiology and Disorders
- Social and Demographic Issues in Germany
- Circular RNAs in diseases
- DNA Repair Mechanisms
- Cellular transport and secretion
University of South Alabama
2021-2023
USA Mitchell Cancer Institute
2021-2023
Medical University of South Carolina
2017-2022
MUSC Hollings Cancer Center
2018-2021
Munzur University
2018
University of Charleston
2017
Inserm
2014-2015
Université de Reims Champagne-Ardenne
2014-2015
University of Liège
2014
Centre National de la Recherche Scientifique
2014
Abstract The epithelial to mesenchymal transition (EMT), a process that is aberrantly activated in cancer and facilitates metastasis distant organs, requires coordinated transcriptional post-transcriptional control of gene expression. tumor-suppressive RNA binding protein, hnRNP-E1, regulates splicing translation EMT-associated transcripts it thought plays major role the cell plasticity during progression. We have utilized yeast 2 hybrid screening identify novel hnRNP-E1 interactors play...
Abstract In many cancers, including lung carcinomas, Fragile histidine triad (Fhit) is frequently decreased or lost. Fhit status has recently been shown to be associated with elevated in vitro and vivo invasiveness cancer. Tumor cell invasion facilitated by epithelial–mesenchymal transition (EMT), a process which tumor cells lose their epithelial features acquire mesenchymal cell-like phenotype. this study, the mechanism underlying Fhit-regulated EMT was deciphered. Using Slug knockdown,...
Zonula occludens-1 (ZO-1) is a submembrane scaffolding protein that may display proinvasive functions when it relocates from tight junctions into the cytonuclear compartment. This article examines functional involvement of ZO-1 in CXCL8/IL-8 chemokine expression lung and breast tumor cells. small interfering RNA cDNA transfection experiments emphasized regulation via pool ZO-1. Luciferase reporter assays highlighted 173-bp region promoter responded to Moreover, by using mutated constructs,...
Metastasis is the leading driver of cancer-related death. Tumor cell plasticity associated with epithelial–mesenchymal transition (EMT), an embryonic program also observed in carcinomas, has been proposed to explain colonization distant organs by primary tumor cells. Many studies have established correlations between EMT marker expression and metastasis vivo. However, longstanding model EMT-transitioned cells disseminating secondary sites still actively debated hybrid states are presently...
We have explored the role of human NANOS3 gene in lung tumour progression. show that is over-expressed by invasive cancer cells and a prognostic marker for non-small cell carcinomas (NSCLCs). expression restricted testis brain regulated epigenetic events. It up-regulated cultured undergoing epithelial - mesenchymal transition (EMT). over-expression NSCLC lines enhances their invasiveness up-regulating EMT, whereas its silencing induces transition. represses E-cadherin at transcriptional...
The aggressive nature of certain cancers and their adverse effects on patient outcomes have been linked to cancer innervation, where neurons infiltrate differentiate within the stroma. Recently we demonstrated how plasticity TGFβ signaling could promote breast innervation that is associated with increased aggressivity. Despite promising potential as a target for anti-cancer therapies, there currently significant lack effective methods study cancer-induced neuronal differentiation, hindering...
The epithelial-mesenchymal transition (EMT), in which cells undergo a switch from polarized, epithelial phenotype to highly motile fibroblastic or mesenchymal is fundamental during embryonic development and can be reactivated variety of diseases including cancer. Spatio-temporally-regulated mechanisms are constantly orchestrated allow adapt their changing environments when disseminating distant organs. Although numerous transcriptional regulatory factors currently well-characterized, the...
Recent studies have placed transfer RNA (tRNA), a housekeeping molecule, in the heart of fundamental cellular processes such as embryonic development and tumor progression. Such discoveries were contingent on concomitant methods able to deliver high-quality tRNA profiles. The present study describes proof concept obtained Escherichia coli (E. coli) for an original analysis platform named SPOt (Streamlined Platform Observing tRNA). This approach comprises three steps. First, E. cultures are...
Heterogeneous nuclear ribonucleoprotein E1 (hnRNP E1) is a tumor suppressor protein that binds site- and structure-specifically to RNA sequences regulate mRNA stability, facilitate alternative splicing, suppress translation on several metastasis-associated mRNAs. Here, we show hnRNP polycytosine-rich DNA tracts present throughout the genome, including those at promoters of oncogenes telomeres monitors genome integrity. It in structure-specific manner. E1-knockdown cells displayed increased...
Transfer RNAs (tRNA) are abundant short non-coding RNA species that typically 76 to 90 nucleotides in length.tRNAs directly responsible for protein synthesis by translating codons mRNA into amino acid sequences.tRNAs were long considered as house-keeping molecules lacked regulatory functions.However, a growing body of evidence indicates cellular tRNA levels fluctuate correspondence varying conditions such cell type, environment, and stress.The fluctuation expression influences gene...
Transfer RNAs (tRNA) are abundant short non-coding RNA species that typically 76 to 90 nucleotides in length. tRNAs directly responsible for protein synthesis by translating codons mRNA into amino acid sequences. were long considered as house-keeping molecules lacked regulatory functions. However, a growing body of evidence indicates cellular tRNA levels fluctuate correspondence varying conditions such cell type, environment, and stress. The fluctuation expression influences gene...
<div>Abstract<p>In many cancers, including lung carcinomas, Fragile histidine triad (Fhit) is frequently decreased or lost. Fhit status has recently been shown to be associated with elevated <i>in vitro</i> and vivo</i> invasiveness in cancer. Tumor cell invasion facilitated by epithelial–mesenchymal transition (EMT), a process which tumor cells lose their epithelial features acquire mesenchymal cell-like phenotype. In this study, the mechanism underlying...
<div>Abstract<p>In many cancers, including lung carcinomas, Fragile histidine triad (Fhit) is frequently decreased or lost. Fhit status has recently been shown to be associated with elevated <i>in vitro</i> and vivo</i> invasiveness in cancer. Tumor cell invasion facilitated by epithelial–mesenchymal transition (EMT), a process which tumor cells lose their epithelial features acquire mesenchymal cell-like phenotype. In this study, the mechanism underlying...
<p>PDF file - 108KB, Supplementary figure 4: Effect of pharmacological inhibitors various signaling pathways on cell invasion induced by Fhit silencing. HBE4-E6/E7 cells were transfected with scrambled or siRNA as described in the "Materials and Methods" section. The invasive capacities transfectants tested a modified Boyden chamber assay presence PD98059 (ERK), PP1 (Src), SB203580 (p38), Y27632 (ROCK), KT5720 (PKA), wortmaninn (PI3K), U73122 (PLC) SP600125 (JNK) inhibitors. A...