Abstract Background Although highly effective, primary failure and loss of response to anti tumor necrosis factors (anti-TNF) alpha in inflammatory bowel disease (IBD) are common associated with poor clinical outcomes. Multiple factors, including genetics, have been involved the TNF therapy. Thus, purpose our study was evaluate impact polymorphism SNPs rs1799724 (TNF alpha), rs767455 (TNFRSF1A), rs1061622 (TNFRSF1B) anti-TNF treatment Tunisian IBD patients. Methods We conducted a prospective...

Background Early identification of cardiotoxicity chemotherapy is crucial. Gene expression considered a promising tool.

Cardiopulmonary bypass (CPB) causes reperfusion injury that when most severe is clinically manifested as a systemic inflammatory response syndrome. The anaesthetic propofol may have anti-inflammatory properties reduce such response. We hypothesised differing effects of and isoflurane on markers in patients having CBP. Forty undergoing elective CPB were randomised to receive either or for maintenance anaesthesia. CRP IL-6, IL-8, HIF-1α (ELISA), CD11 CD18 expression (flow cytometry),...

Background/Aims: The most appropriate route for bone marrow-derived mesenchymal stem cell (BM-MSC) transplantation in the management of liver fibrosis remains controversial. This study investigated therapeutic efficacy intravenous and intrasplenic BM-MSC on carbon tetrachloride (CCl4)-induced rat fibrosis. Methods: Fifty rats were divided into 5 groups (n = 10 per group): healthy control group, CCl4 CCl4/ recovery CCl4/BM-MSC group. BM-MSCs isolated, labeled with green fluorescent protein...

Summary It was the aim of this study to determine plasma haemoxygenase-1 (HO-1) across spectrum health, angina but normal coronary arteries (NCA), stable artery disease (CAD), and acute syndromes (ACS), relationships with angiogenin, matrix metalloproteinase-9 (MMP-9), tissue inhibitor metalloproteinase-1, vascular endothelial growth factor. Plasma markers were measured (ELISA) in peripheral venous citrated from 50 healthy subjects, 30 NCA, 70 CAD 24 an ACS, patient’s aortic root, ostium,...

Data about plasma levels of neutrophil gelatinase-associated lipocalin (NGAL) in children with heart failure (HF) are very limited. NGAL is used widely as a biomarker for the diagnosis renal injury numerous clinical studies. The aim this study to investigate HF caused by idiopathic dilated cardiomyopathy (IDCM) and its relation severity HF.In case-control study, 30 nondiabetic children, aged -16 years (all have IDCM) recruited from pediatric department our institute together healthy were...

Hypercoagulability in mitral valve disease (MVD), a cause of atrial fibrillation (AF) and stroke, is potentially due to endothelial damage/dysfunction (marked by circulating cells [CECs]), platelet activation (soluble P-selectin [sPsel], microparticles [PMPs], soluble CD40 [sCD40]), oxidized low-density lipoprotein (oxLDL) cholesterol. We measured these variables 24 patients with MVD as well 21 + AF compared them 20 healthy controls (HCs). The CECs PMPs were flow cytometry; sPsel, oxLDL,...

Hepatocellular carcinoma (HCC) is one of the commonest tumors and considered fifth most common malignant neoplasm a major cause death. Egypt has increased incidence HCC cases, as highest prevalence hepatitis C virus (HCV) infection. The aim was to study epidemiological characteristics in Assiut, Egypt. A descriptive observational design applied for present study. studied population 400 patients with (288 them were male 112 female) who fulfilled diagnostic criteria HCC. Data analyzed...

Background/Aims: Hepatocellular carcinoma (HCC) is the fifth most common malignancy and frequently develops in patients with cirrhosis.Surveillance strategies are recommended high-risk groups because early detection of small lesions improves likelihood curative treatment.This study investigated prospective clinical significance serum levels anti-Ku86 plasma lamin B1and vimentin as markers HCC.Methods: We recruited 74 at Assiut University Hospital-37 HCC 37 chronic liver disease (liver...

Background Interleukin-18 (IL-18) and interferon-γ (IFN-γ) are cytokines of crucial role in inflammation immune reactions. There is a growing evidence supporting important roles for IL-18 IFN γ tuberculosis (TB) infection anti-tuberculosis immunity. Objective To evaluate the polymorphisms IL-18-607 -137 INF-γ +874 susceptibility to TB among Egyptian patients. Methods A case control study was conducted investigate polymorphism at IL-18-607, INF-γ+874 by sequence specific primer-polymerase...

Background: Most clinical trials of sepsis treatment modalities fail at their primary objective establishing superiority over placebo when added to background standard care.While there is no definitive explanation for the high failure rate, it might be stated that our attempts insert a new therapeutic agent into care encounters severe problems with definition exactly what stage ongoing, and are criteria progression or resolution from time point onwards.Clearly need means defining steps in...

Rheumatoid arthritis (RA) is the most widespread chronic inflammatory rheumatic disease over world. It characterized by proliferation of synovium, cartilage destruction, and periarticular erosion/bone loss. We investigated serum levels C-telopeptide type II collagen (CTX-II), Dickkopf-1 (DKK1), oligomeric matrix protein (COMP) in relationship to activity.Serum COMP, CTX-II, DKK1 were measured 63 RA patients 50 person age gender matched as a healthy controls ELISA test. Disease activity score...

Abstract We investigated the whole blood GLUT1 mRNA expression and serum pigment epithelium-derived factor (PEDF), interleukin-6 (IL-6), fetuin-A, pentraxin-3 (PTX3) levels in psoriatic patients tested their correlations with severity of psoriasis using area index (PASI) score. Also, we after an vitro treatment human skin fibroblast (HSF) cell lines PEDF. The case–control part study recruited 74 participants (44 30 healthy volunteers). Whole fold changes were estimated by RT-PCR, PEDF, IL-6,...

BACKGROUND & AIM. The mechanisms by which type 2 diabetes mellitus (T2DM) worsen liver function are not yet established. Tissue factor (TF) is a protein that participates in hemostatic, immune and inflammatory processes. To test the hypothesis T2DM contributes to clinical outcome through changes of TF expression on monocytes investigate association between antidiabetic therapies monocytic HCV-related cirrhotic patients with T2DM. MATERIAL AND METHODS. In (139 diabetics 130 non diabetics)...

Ultrasound-guided supraclavicular brachial plexus block (BPB) has come into wider use as a regional anesthetic during upper limb operations. This study assessed the neurological and hemodynamic changes gene expression after co-administration of midazolam or neostigmine with bupivacaine BPB.The involved 90 adults divided three groups: control (bupivacaine), (bupivacaine plus midazolam), neostigmine). Blood samples were taken interleukin-6 (IL-6) tumor necrosis factor-alpha (TNF-α) mRNA levels...

Rheumatoid arthritis (RA) is one of the most widespread autoimmune disorders and it has a genetic background with variety genes affecting degradation immune system. Along these lines, we assessed relationship between BsmI, FokI VDR polymorphisms inflammable records identified infections activity. Such as interleukins (IL-6, IL-8), hypoxia inducible factor-alpha (HIF-α), soluble receptor advanced glycation end product (sRAGE), oxidized low-density lipoprotein cholesterol (oxLDL), neutrophil...

Human umbilical cord blood (UCB) cells and rat bone marrow mesenchymal stem (BM-MSCs) have many advantages as grafts for cell transplantation.The aim of this study was to investigate the impact UCB BM-MSCs on reversal hepatic injury revival function in a model carbon tetrachloride (CCl 4 )-induced liver fibrosis.Keywords: CCl fibrosis,

Background/Aims: Genetic alterations, including changes in the expression of spastic paraplegia 20 (SPG20) and serine/threonine protein kinase 31 (STK31), may play an important role carcinogenesis colorectal cancer (CRC). Identification such is suitable for recognition tumors at early stage, which would significantly improve patient survival. While recent studies have identified that SPG20 STK31 levels increase CRC tissues, their use as a biomarker yet to be investigated. Our aim was...