We previously reported that lifetime consumption of soy proteins or whey reduced the incidence azoxymethane (AOM)-induced colon tumors in rats. To obtain insights into these effects, global gene expression profiles colons from rats with ingestion casein (CAS, control diet), protein isolate (SPI), and hydrolysate (WPH) diets were determined. Male Sprague Dawley rats, fed one three purified diets, studied at 40 weeks after AOM injection when had developed some animals each group. Total RNA,...

In order to facilitate studies of insulin-like growth factor-I (IGF-I) expression during the pregnancyassociated development uterus and mammary gland in pig model, we have isolated several cDNA clones corresponding porcine IGF-I (pIGFI) mRNA. Sequence analysis two fragments (sigf.2 sigf.3) revealed an open reading frame encoding a putative 25 amino acid (aa) hydrophobic leader peptide, mature (processed) 70 aa pIGF-I peptide 35 carboxy-terminal extension (E) peptide. The deduced sequence is...

This study was conducted to identify polymorphisms in the promoter and coding regions of bovine growth hormone receptor genes association identified these with traits serum insulin-like factor-I (IGF-I) concentration. The denaturing gradient gel electrophoresis method sequencing were utilized three new single nucleotide region gene Angus cattle. Polymerase chain reaction-based restriction fragment length polymorphism procedures developed for rapid determination genotypes among calves from...

The present study characterized the changes in concentrations of insulin-like growth factors-I and -II (IGF-I IGF-II) uterine luminal flushings, endometrial mRNA levels IGF-I, IGF-II, IGF binding protein-2 (IGFBP-2) obtained from Days 0 through 18 bovine estrous cycle early pregnancy. Concentrations IGF-I IGF-II flushings were greater on 5 than other days or Northern blot analysis poly(A)+ RNA revealed two major transcripts 7.5 1.0 Kb for one transcript 4.0 1.3 IGFBP-2. dot-blot analyses...

The present studies were undertaken to define potential regulation of endometrial insulin-like growth factor-I (IGF-I) synthesis and secretion by steroid hormones correlate conceptus- serum-derived estrogens with these events. Four experimental groups gilts studied: 1) prepubertal administered estradiol (E) and/or progesterone (P4) daily for up 15 days; 2) mature ovariectomized (ovx) on day 2 the estrous cycle, then E, P4, or E plus P4 from days 4–11 after estrus; 3) unilaterally pregnant 12...

Breast cancer risk is highly modifiable by diet; however, mechanisms underlying dietary protection against mammary tumorigenesis remain poorly understood. A proportion of breast carcinomas associated with deregulation β-catenin stability and amplification c-Myc expression. We recently showed that exposure to the soy isoflavone genistein (Gen) inhibited Wnt transduction in rat epithelial cells vivo . Here, we explored role Gen on cell adhesion protein, E-cadherin, expression downregulate...

Endometriosis is characterized by progesterone resistance and associated with infertility. Krüppel-like factor 9 (KLF9) a receptor (PGR)-interacting protein, mice null for Klf9 are subfertile. Whether loss of KLF9 expression contributes to eutopic endometrium women endometriosis unknown.The aims were investigate 1) in without endometriosis, 2) effects attenuated on WNT-signaling component WNT inhibitor Dickkopf-1 promoter activity human endometrial stromal cells (HESC), 3) PGR coregulation...

The effects of growth hormone (GH) and pregnancy on insulin-like factor (IGF)-I, IGF binding protein (IGFBP)-2, IGFBP-3 mRNA in reproductive tissues were studied cattle. Lactating dairy cows inseminated at estrus treated with 25 mg/day GH (n = 8) or saline for 16 days. Corpus luteum (CL), ovary (CL removed), oviduct, endometrium, myometrium collected the end treatment. Messenger RNA receptor, IGF-I, IGFBP-2, IGFBP-3, actin measured by nuclease protection assays. CL contained more receptor...

The insulin-like growth factors (IGF-I and IGFII) circulate in plasma association with IGF-binding proteins (IGFBPs). As a first step to understanding the regulation of expression these pigs, we characterized ontogeny circulating IGFs IGFBPs during fetal early postnatal development. Serum were separated from IGFBPs, before IGF RIA, by acidification chromatography on C18 Sep-Pak cartridges. IGF-I levels increased latter half life 11 ± 1 ng/ml day 60 37 3 112 (2-3 days term) further...

Estradiol (E2)-active (Day 5 ID5]), transitional E2-active (D8), atretic (D12-1), and E2-sustained dominant follicles (DF, D12+1) associated subordinate (SF) were obtained through use of an experimental model described here. The ovary bearing the DF was surgically removed by colpotomy, individual utilized to study changes in concentrations insulin like growth factor-I (IGF-I) -II (IGF-II) amounts proportions different IGF-binding proteins (IGFBP) present follicular fluid (FF). ratio FF E2...

Data for this study were obtained from an experiment involving divergent selection blood serum IGF-I concentration in beef cattle. Multiple trait derivative-free REML procedures used to obtain genetic parameter estimates at d 28, 42, and 56 of the postweaning period mean concentration, as well weights gains. Included analysis 1,563 animals A-1 matrix, 731 which had valid records concentration. Direct heritabilities (hd2d) .42 ± .13, .53 .15, .71 .16, .48 .13 IGF-I, respectively. Heritability...

Abstract The type 5, iron-containing, tartrate-resistant acid phosphatase (TR-AP) constitutes a relatively minor intracellular isozyme of in the human that is immunologically related to uteroferrin, secreted progesterone-induced protein porcine uterus. Here, purification small amounts TR-AP from placenta described. When placental lambda gt11 cDNA library was screened with two short 32P-labeled clones within coding region 1412-base pair identified encodes entire isozyme. This contains an open...

The temporal patterns of endometrial expression for mRNAs encoding insulin-like growth factor-I (IGF-I), IGF-II, IGF-binding protein-2 (IGFBP-2), and the type I IGF receptor (IGF-IR) were elucidated in cyclic pregnant pigs. Peak levels IGF-I occurred on day 12 early gilts, while IGFBP-2 mRNA lowest 10. Pregnant gilt endometrium had higher both RNA classes than corresponding endometrium. IGF-II IGF-IR remained low during this period. In pig rat uterus, decreased, those increased with stage...

Progesterone receptor (PR), a ligand-activated transcription factor, is key regulator of cellular proliferation and differentiation in reproductive tissues. The transcriptional activity PR influenced by co-regulatory proteins typically expressed tissue- cell-specific fashion. We previously demonstrated that basic element-binding protein-1 (BTEB1), member the Sp/Krüppel-like family factors, functionally interacts with two isoforms, PR-A PR-B, to mediate progestin sensitivity target genes...

Uterine luminal fluids (ULFs) from Days 10, 12, 14, and 16 cyclic (C) pregnant (Px) ewes were analyzed for presence of insulin-like growth factors (IGF-I, IGF-II) other mitogenic factor(s). IGF content non-IGF activity measured by RIAs after removal binding proteins stimulatory effects on DNA synthesis density arrested AKR-2B cells, respectively. ULF IGF-I was not different between days, but differences in C Px groups at Day (C greater than Px) found (p < 0.05). IGF-II ewes; however, among...

Journal Article Perinatal Expression of Type I IGF Receptors in Porcine Small Intestine Get access DOUGLAS A. SCHOBER, SCHOBER 1Department Animal Science (D.A.S., F.A.S.), Ohio Agricultural Research and Development Center. The State UniversityWooster, OH 44691; Department Dairy Science, Pennsylvania University (D.L.H., C.R.B.)University Park, PA 16802 Search for other works by this author on: Oxford Academic Google Scholar FRANK SIMMEN, SIMMEN DARRYL L. HADSELL, HADSELL CRAIG R. BAUMRUCKER...

The structural organization of the entire chicken calmodulin (CaM) gene was determined by analysis overlapping genomic clones obtained from Charon 4A and cosmid DNA libraries. These together span 39 kilobases DNA. CaM is 12 long contains 8 exons interrupted introns highly variable size. first intron (A) only 19 base pairs (bp) divides 5' untranslated region. Intron B separates ATG nucleotide triplet which encodes NH2-terminal amino acid (Ala) whereas C triplets encoding acids 10-11. Introns...

The role of diet in the prevention breast cancer is widely accepted, yet little known about how its biological effects mitigate susceptibility to this disease. Soy consumption associated with reduced risk women, an effect largely attributed soy isoflavone genistein (Gen). We previously showed incidence chemically induced mammary tumors young adult rats lifetime dietary intake protein isolate (SPI) than those fed control containing casein (Cas). To gain insight into signaling pathways...