A5075946064- Growth Hormone and Insulin-like Growth Factors
- Digestive system and related health
- Animal Genetics and Reproduction
- Infant Nutrition and Health
- Metabolism, Diabetes, and Cancer
- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- Cancer-related Molecular Pathways
- Breastfeeding Practices and Influences
- Clinical Nutrition and Gastroenterology
- Genetic Mapping and Diversity in Plants and Animals
- Reproductive Physiology in Livestock
- Genetic and phenotypic traits in livestock
- Virus-based gene therapy research
- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
- Neonatal Respiratory Health Research
- Mitochondrial Function and Pathology
- Cancer Risks and Factors
- Estrogen and related hormone effects
- Birth, Development, and Health
- Carcinogens and Genotoxicity Assessment
- Adipose Tissue and Metabolism
- Metabolism and Genetic Disorders
- Pancreatic function and diabetes
Children's Nutrition Research Center at Baylor College of Medicine
2009-2025
Baylor College of Medicine
2006-2018
United States Department of Agriculture
2002-2016
Agricultural Research Service
2001-2016
Arkansas Children's Nutrition Center
2002-2010
Texas Children's Hospital
2010
Center for Cancer Research
2003
Breast Center
2002
Pennsylvania State University
1990-1994
Allegheny-Singer Research Institute
1990
The insulin-like growth factor I receptor (IGF-IR) is a transmembrane tyrosine kinase that essential to and development also thought provide survival signal for the maintenance of transformed phenotype. There has been increasing interest in further understanding role IGF-I signaling cancer developing antagonists therapeutic application. We describe herein novel animal model involves transgenic expression fusion constitutively activated by homodimerization. Transgenic mice expressed showed...
This paper resulted from a conference entitled "Lactation and Milk: Defining refining the critical questions" held at University of Colorado School Medicine January 18–20, 2012. The mission was to identify unresolved questions set future goals for research into human milk composition, mammary development lactation. We first outline unanswered regarding composition (Section I) mechanisms by which components affect neonatal development, growth health recommend models research. Emerging about...
Insulin receptor substrates (IRSs) are signaling adaptors that play a major role in the metabolic and mitogenic actions of insulin insulin-like growth factors. Reports have recently noted increased levels, or activity, IRSs many human cancers, some linked this to poor patient prognosis. We found overexpressed IRS-1 was constitutively phosphorylated vitro vivo transgenic mice overexpressing IRS-2 mammary gland showed progressive hyperplasia, tumorigenesis, metastasis. Tumors extensive...
The molecular physiology underlying human milk production is largely unknown because of limitations in obtaining tissue samples. Determining gene expression normal lactating women would be a potential step toward understanding why some struggle with or fail at breastfeeding their infants. Recently, we demonstrated the utility RNA obtained from breast fat globule (MFG) to detect mammary epithelial cell (MEC)-specific expression. We used MFG determine profile MEC during lactation. Microarray...
To test the hypothesis that insulin-like growth factor I (IGF-I) regulates mammary gland development and lactation, expression of both human (h) IGF-I des(1-3)hIGF-I was targeted to in transgenic mice using a novel exon replacement strategy rat whey acidic protein (rWAP) gene regulatory sequences. Both transgenes expressed 0.7-kilobase messenger RNA (mRNA). The abundance WAP-IGE-I WAP-DES mRNA on day 10 lactation ranged from 0.2-1.0% 0.2-13% endogenous mouse WAP mRNA, respectively. For mice,...
Colostrum is rich in IGF-I and IGF-II, the dietary effects of recombinant human (rh)IGF-I on newborn are interest. The objective this study was to examine rhIGF-I intestinal tissue growth populations IGF receptors. Twenty-three male dairy calves were fed one three experimental diets: 1) milk replacer plus isolated colostrum-derived globulins (MR−), 2) same as 1 750 ng rhIGF-I/mL (MR+), or 3) pooled cow colostrum (COL). After first four feedings, all received without additional globulins; MR+...
IGF-I mediates mammary ductal development through stimulation of terminal end bud (TEB) development; however, no published data exist on the mechanism which this occurs. The action TEB was studied by determining requirement for receptor (IGF-IR) in IGF-I-dependent development. We hypothesized that loss IGF-IR would disrupt a combination decreased proliferation or increased apoptosis. Because null mice die at birth, embryonic gland transplantation used to study effects disrupted gene....
Prolactin (PRL) is a major determinant of mammary epithelial cell proliferation during alveolar development in sexually mature and pregnant mice. To date, it has not been clear whether PRL effects these responses alone or by also invoking the action autocrine/paracrine growth factors. In this study, we provide evidence that part effect on gland mediated IGF-II. During sexual maturity early pregnancy, level IGF-II mRNA was increased concurrent with receptor expression. The reduced tissue from...
Concentrations of IGF-I and IGF-II, IGF binding proteins (IGFBP) in serum mammary gland secretions were surveyed during the dry period early lactation 30 Holstein cows. Although there was a threefold drop concentration from last week to parturition (81 ± 7 24 3 ng/ml, P < .01), no significant change IGF-II this (150 17 vs 173 ±13 > .05). Furthermore, 57% increase observed second drying off (100 5 157 8 Changes not (126 11 150 10 respectively; IGF-I, IGFBP concentrations peaked 2 wk before...
Journal Article Perinatal Expression of Type I IGF Receptors in Porcine Small Intestine Get access DOUGLAS A. SCHOBER, SCHOBER 1Department Animal Science (D.A.S., F.A.S.), Ohio Agricultural Research and Development Center. The State UniversityWooster, OH 44691; Department Dairy Science, Pennsylvania University (D.L.H., C.R.B.)University Park, PA 16802 Search for other works by this author on: Oxford Academic Google Scholar FRANK SIMMEN, SIMMEN DARRYL L. HADSELL, HADSELL CRAIG R. BAUMRUCKER...
Lactose synthesis is believed to be rate limiting for milk production. However, understanding the molecular events controlling lactose in humans still rudimentary. We have utilized our established model of RNA isolated from breast fat globule seven healthy, exclusively breastfeeding women 6 h 42 days following delivery determine temporal coordination changes gene expression carbohydrate metabolic processes emphasizing pathway human mammary epithelial cell. showed that concentrations...
During pregnancy the mammary epithelium and its supporting vasculature rapidly expand to prepare for lactation, resulting in dramatic changes micro-environment. In order investigate role of oxygenation metabolism these processes, oxygen-responsive component hypoxia-inducible factor (HIF) 1 complex, HIF1α, was deleted murine gland. Although vascular density unchanged HIF1α null gland, loss impaired differentiation lipid secretion, culminating lactation failure striking milk composition....
We previously demonstrated the dose-dependent glucagon-like peptide (GLP)-2 activation of intracellular signals associated with increased epithelial cell survival and proliferation in neonatal intestine. Our current aim was to quantify acute, temporal GLP-2 these key relate this changes studied 29 total parenteral nutrition-fed piglets infused intravenously either saline (control) or human (420 micromol.kg(-1).h(-1)) for 1, 4, 48 h. infusion small intestinal weight, DNA protein content,...
Abstract Previous studies from our laboratory have demonstrated that p190-B RhoGAP (p190-B) is differentially expressed in the Cap cells of terminal end buds (TEBs) and poorly differentiated rodent mammary tumors. Based on these observations we hypothesized might play an essential role invasion TEBs into surrounding fat pad during ductal morphogenesis. To test this hypothesis, development was studied p190-B-deficient mice. A haploinsufficiency phenotype observed heterozygous mice as...