A5028222365- Insect Resistance and Genetics
- CRISPR and Genetic Engineering
- Insect behavior and control techniques
- Viral Infectious Diseases and Gene Expression in Insects
- Insect symbiosis and bacterial influences
- Insect and Pesticide Research
- Forensic Entomology and Diptera Studies
- Insect Utilization and Effects
- Neurobiology and Insect Physiology Research
- Genomics and Chromatin Dynamics
- Animal Genetics and Reproduction
- Insect-Plant Interactions and Control
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- RNA Research and Splicing
- Invertebrate Immune Response Mechanisms
- Plant Virus Research Studies
- Mosquito-borne diseases and control
- Histone Deacetylase Inhibitors Research
- Ubiquitin and proteasome pathways
- Chromosomal and Genetic Variations
- Heat shock proteins research
- Animal Behavior and Reproduction
- RNA and protein synthesis mechanisms
- Epigenetics and DNA Methylation
- T-cell and Retrovirus Studies
North Carolina State University
2015-2024
Massey University
2002-2012
Emory University
1995
University of Alabama in Huntsville
1994
University of North Carolina at Chapel Hill
1991
Baylor College of Medicine
1987
Purdue University Northwest
1987
University West
1987
Tsetse flies are the sole vectors of human African trypanosomiasis throughout sub-Saharan Africa. Both sexes adult tsetse feed exclusively on blood and contribute to disease transmission. Notable differences between other include obligate microbial symbioses, viviparous reproduction, lactation. Here, we describe sequence annotation 366-megabase Glossina morsitans genome. Analysis genome 12,308 predicted protein-encoding genes led multiple discoveries, including chromosomal integrations...
Drosophila suzukii (Matsumura) (Diptera: Drosophilidae) also known as spotted-wing drosophila (SWD), is a pest native to Southeast Asia. In the last few decades, has expanded its range affect all major European and American fruit production regions. SWD highly adaptive insect that able disperse, survive, flourish under of environmental conditions. Infestation by generates both direct indirect economic impacts through yield losses, shorter shelf life infested fruit, increased costs. Fresh...
Genetic-based methods offer environmentally friendly species-specific approaches for control of insect pests. One method, CRISPR homing gene drive that target genes essential development, could provide very efficient and cost-effective control. While significant progress has been made in developing drives mosquito disease vectors, little with agricultural Here, we report the development evaluation split doublesex (dsx) Drosophila suzukii, an invasive pest soft-skinned fruits. The component,...
We have developed a tetracycline-repressible female-specific lethal genetic system in the vinegar fly Drosophila melanogaster. One component of is tetracycline-controlled transactivator gene under control fat body and transcription enhancer from yolk protein 1 gene. The other consists proapoptotic hid tetracycline-responsive element. Males females strain carrying both components are viable on medium supplemented with tetracycline, but only males survive normal medium. A such properties would...
Genetic technologies such as gene editing and drive systems have recently emerged potential tools for pest control. Gene drives, in particular, been described solutions to the problems that beset New Zealand. Here we describe current state of present a series examples examine benefits arising from approaches control We consider risks barriers, both biological social, would need be addressed deploy against our key pests with particular reference unique characteristics Zealand's biota,...
We must ensure that trials are scientifically, politically, and socially robust, publicly accountable, widely transparent
Insects use an amazing variety of genetic systems to control sexual development. A Y-linked male determining gene (M) controls sex in the Australian sheep blowfly Lucilia cuprina, important pest insect. In this study, we isolated L. cuprina transformer (Lctra) and transformer2 (Lctra2) genes, which are potential targets M. The LCTRA LCTRA2 proteins significantly more similar homologs from tephritid insects than Drosophila. Lctra transcript is alternatively spliced such that only females make...
Parasitism is an important life history strategy in many metazoan taxa. This particularly true of the Phylum Nematoda, which parasitism has evolved independently at least nine times. The apparent ease with amongst nematodes may, part, be due to a feature nematode development acting as pre-adaptation for transition from free-living parasitic history. One candidate pre-adaptive evolution terrestrial dauer larva, developmentally arrested morph formed response environmental signals.We...
To highlight how gene drives could be useful for control of agricultural insect pests, we selected species that are pests animals (New World screwworm), plants (spotted wing Drosophila, diamondback moth, Bemisia tabaci whitefly), or stored grains (red flour beetle). With the exception whitefly, routine methods delivering DNA to germline and selecting genetically modified insects have been developed. The traditional approach in agriculture has suppress pest populations using insecticides...
A growing body of research indicates that pharmacological inhibition histone deacetylases (HDACs) correlates with enhancement long-term memory and current is concentrated on determining the roles individual HDACs play in cognitive function. Here, we investigate role HDAC4 formation Drosophila. We show overexpression adult mushroom body, an important structure for formation, resulted a specific impairment courtship memory, but had no affect short-term memory. Overexpression catalytic mutant...
The New World screwworm, Cochliomyia hominivorax, is a devastating pest of livestock endemic to subtropical and tropical regions the Western hemisphere. larvae this species feed on tissue living animals, including man, can cause death if untreated. Over 60 years ago, sterile insect technique (SIT) was developed with aim eradicating pest, initially from Florida but subsequently all North Central America. From outset it appreciated that SIT would be more efficient only males were released in...
Diabetes and its concurrent complications impact a significant proportion of the population US create large financial burden on American health care system. FDA-approved maggot debridement therapy (MDT), application sterile laboratory-reared Lucilia sericata (green bottle fly) larvae to wounds, is cost-effective successful treatment for diabetic foot ulcers other medical conditions. Human platelet derived growth factor-BB (PDGF-BB) secreted dimeric peptide factor that binds PDGF receptor....
Abstract New World screwworms, C ochliomyia hominivorax ( oquerel) D iptera: alliphoridae: hrysomyinae), are devastating pests of warm‐blooded animals that cause significant economic damage to livestock. The successful campaign eradicate screwworms from continental N orth A merica, led by the US Department Agriculture and using sterile insect technique, continues receive research support has resulted in improved technologies for all aspects program. process ingredients mass‐rearing is more...
The TAN-1 gene was originally discovered at the breakpoint of a recurrent (7;9)(q34;q34.3) chromosomal translocation found in subset human T-lymphoblastic leukemias (Reynolds et al. 1987; Smith 1988; Ellisen 1991). This joins roughly 3′ half head-to-head with portion β T-cell-receptor (TCRB) beginning 5′ boundary one or other J segment. Intact is normally transcribed into an 8.2-kb transcript that present many tissues, most abundantly developing thymus and spleen (Ellisen tissue distribution...
The structural organization of the entire chicken calmodulin (CaM) gene was determined by analysis overlapping genomic clones obtained from Charon 4A and cosmid DNA libraries. These together span 39 kilobases DNA. CaM is 12 long contains 8 exons interrupted introns highly variable size. first intron (A) only 19 base pairs (bp) divides 5' untranslated region. Intron B separates ATG nucleotide triplet which encodes NH2-terminal amino acid (Ala) whereas C triplets encoding acids 10-11. Introns...
Transformer (TRA) promotes female development in several dipteran species including the Australian sheep blowfly Lucilia cuprina, Mediterranean fruit fly, housefly and Drosophila melanogaster. tra transcripts are sex-specifically spliced such that only form encodes full length functional protein. The presence of six predicted TRA/TRA2 binding sites sex-specific intron L. cuprina gene suggested splicing is auto-regulated as medfly housefly. With aim identifying conserved motifs may play a...
Abstract Genetic approaches, including the sterile insect technique (SIT), have previously been considered for control of Australian sheep blow fly Lucilia cuprina , a major pest sheep. In an SIT program, females consume 50% diet but are ineffective as agents and compete with in field mating males, thereby decreasing efficiency program. Consequently, transgenic sexing strains L. were developed that produce 100% males when raised on lacks tetracycline. However, die mostly at pupal stage,...
The Drosophila suzukii invasion of western countries has created an immense agricultural and economic threat to crop production. Despite many attempts suppress its population, D. continues destroy soft-flesh fruits. Precision guided sterile insect technique (pgSIT) utilizes the accuracy programmable CRISPR gene targeting generate sterilized males that can be deployed populations. Here, we pgSIT in empirically mathematically demonstrate are fit, competitive, eliminate populations suzukii....
We have isolated cDNA clones and determined the gene structure of chicken ovoinhibitor, a seven domain Kazal serine proteinase inhibitor. Using RNA blot hybridization analysis, was identified initially as region 9-23 kilobases upstream for related inhibitor ovomucoid. Ovoinhibitor appears in oviduct liver. were by screening an library with nick-translated DNA restriction fragment which contained exon gene. The mature protein sequence derived from cDNa clone is excellent agreement that we...