A5105349884- Insect Resistance and Genetics
- Iron Metabolism and Disorders
- Mosquito-borne diseases and control
- Insect symbiosis and bacterial influences
- Invertebrate Immune Response Mechanisms
- Insect Utilization and Effects
- Endoplasmic Reticulum Stress and Disease
- Studies on Chitinases and Chitosanases
- Entomopathogenic Microorganisms in Pest Control
- Trypanosoma species research and implications
- Malaria Research and Control
- Insect Pest Control Strategies
- Celiac Disease Research and Management
- Enzyme Production and Characterization
- Phytoplasmas and Hemiptera pathogens
- Trace Elements in Health
- Insect and Pesticide Research
- Retinal Development and Disorders
- Hemoglobinopathies and Related Disorders
- Insect-Plant Interactions and Control
University of Arizona
2012-2022
Arizona Science Center
2003
Tsetse flies are the sole vectors of human African trypanosomiasis throughout sub-Saharan Africa. Both sexes adult tsetse feed exclusively on blood and contribute to disease transmission. Notable differences between other include obligate microbial symbioses, viviparous reproduction, lactation. Here, we describe sequence annotation 366-megabase Glossina morsitans genome. Analysis genome 12,308 predicted protein-encoding genes led multiple discoveries, including chromosomal integrations...
Diseases transmitted by hematophagous (blood‐feeding) insects are responsible for millions of human deaths worldwide. In insects, the blood meal is important regulating egg maturation. Although a high concentration iron toxic most organisms, seem unaffected load in meal. One means which handle this is, perhaps, expression iron‐binding proteins, specifically storage protein ferritin. vertebrates, ferritin an oligomer composed two types subunits called heavy and light chains, part constitutive...
Secreted ferritin is the major iron storage and transport protein in insects. Here, we characterize message expression profiles of yellow fever mosquito (Aedes aegypti) heavy chain homologue (HCH) light (LCH) subunits response to bacterial challenge. In vivo experiments demonstrated tissue-specific regulation HCH LCH over time post-blood meal (PBM). Transcriptional was treatment specific, with differences for naïve versus mosquitoes challenged heat-killed bacteria (HKB). Translational by...
Ferritin is a 24-subunit molecule, made up of heavy chain (HC) and light (LC) subunits, which stores controls the release dietary iron in mammals, plants, insects. In mosquitoes, taken bloodmeal stored inside ferritin. Our previous work has demonstrated transport to ovaries via ferritin during oogenesis. We evaluated localization subunits CCL-125 [ Aedes aegypti Linnaeus (Diptera: Culicidae), yellow fever mosquito] 4a3b Anopheles gambiae Giles African malaria cells under various treatment...
Abstract Dengue, yellow fever, and Zika are viruses transmitted by fever mosquito, Aedes aegypti [Linnaeus (Diptera: Culicidae)], to thousands of people each year. Mosquitoes transmit these while consuming a blood meal that is required for oogenesis. Iron, an essential nutrient from the meal, egg development. receive high iron load in meal; although can be toxic, animals have developed mechanisms dealing with this load. Our previous research has shown absorbed gut transported ferritin, main...
Amylases are an important family of enzymes involved in insect carbohydrate metabolism that required for the survival larvae. For this reason, from starch-dependent insects targets insecticidal control. PF2 (Olneya tesota) is a lectin toxic to Zabrotes subfasciatus (Coleoptera: Bruchidae) In study, we evaluated recognition α-amylases Z. midgut and effect on α-amylase activity. caused decrease total amylase activity vitro. Subsequently, several isoforms were isolated tissues using ion...
Zabrotes subfasciatus (Boheman) is the main pest of common beans ( Phaselous vulgaris ). Wild legume seeds from Olneya tesota contain a lectin, PF2, that shows insecticidal activities against this insect. The binding PF2 to midgut glycoproteins 20-day-old larvae was evaluated using affinity chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis proteins retained on revealed several putative glycoproteins, ranging in mass 17 97 kDa. Subsequent protein...
Ferritin is required for iron storage in vertebrates and transport invertebrates, specifically insects. Classical ferritins consist of 24 subunits configured as a polyhedron wherein held. The include light heavy chains, each with specific functions. Several homologues the chains have been sequenced studied In addition to storage, ferritin has role dietary absorption, functions protective agent preventing overload, decreasing oxidative stress, reducing infection these animals. expression...
The available genomic and proteomic information of non-model organisms is often underrepresented in public databases hindering their study at molecular, cellular, physiological levels. Information on Zabrotes subfasciatus (Mexican bean weevil) poorly represented databases, yet it a major pest common beans. We report the transcriptome Z. larvae; transcripts were sequenced using an Illumina RNA-Seq technology assembled de novo identifying 29,029 unigenes with average size 1168 bp N50 value...
Aedes aegypti [Linnaeus in Hasselquist; yellow fever mosquito] transmits several viruses that infect millions of people each year, including Zika, dengue, fever, chikungunya, and West Nile. Pathogen transmission occurs during blood feeding. Only the females feed as they require a bloodmeal for oogenesis; bloodmeal, holo-transferrin hemoglobin provide with high iron load. We are interested effects on expression iron-associated proteins oogenesis. Previous data showed following digestion...
Diseases transmitted by mosquitoes are estimated to kill more than 2 million people annually. The vector for dengue, Aedes aegypti (yellow fever mosquito), is found in the desert Southwest of continental United States. One approach limiting spread disease reduce numbers mosquito vectors. Our area research iron metabolism. long term goal use molecular techniques alter expression related proteins fecundity and survival. hypothesis that female require blood meal as an essential nutrient aid egg...
Insect midguts, as well other animals, are rich in glycoconjugates that have important biological functions required for growth and development. Dietary lectins can bind to gut induce various insecticidal effects such blocking of receptors or transporters inactivation activation enzymes. PF2 is a lectin isolated from the seeds Olneya tesota (Palo Fierro), reported cause 100% mortality Zabrotes subfasciatus larvae (Mexican bean weevil). We evaluated recognition by α‐amylases Z. larval midgut...
Malaria and yellow fever are transmitted from mosquitoes to humans through a mosquito bite. Mosquitoes require the essential nutrients blood meal, including iron, for oogenesis. Very little research has been conducted elucidate effect of iron on fecundity. Understanding mechanism deposition in ovary is important finding potential strategies interfere with fecundity, decrease populations, reduce transmission rates vector‐borne diseases. To this end, Aedes aegypti (yellow mosquito) ovaries...
Female mosquitoes require a blood meal for oogenesis and receive substantial iron load in this the form of holo‐transferrin hemoglobin. During course digestion absorption 72 hours post feeding, concentration gut decreases 10‐fold while that ovaries more than doubles. Eggs are laid with ~125 ng Fe each; majority coming from meal. Iron is mitogenic deposition may induce changes endogenous protein expression profile over time. Proteomic analysis extracts developing eggs revealed proteins...
Dengue and Yellow Fever are caused by viruses transmitted mosquitoes that each year infect 390 million 200,000 people, respectively, cause more than 2 deaths. For mosquito eggs to develop produce viable offspring, iron is required as an essential nutrient from the blood meal. Our previous research has shown meal absorbed transported ferritin midgut ovaries in Aedes aegypti (yellow fever mosquito). Thus, understanding accumulation utilization important finding potential strategies interfere...
Ferritin is a 24‐subunit molecule, made up of heavy chain (HCH) and light (LCH) homologues that stores controls the release dietary iron in mammalian, plant, insect systems. Dietary taken blood meal stored inside ferritin, previous work mosquitoes demonstrates an important function for process egg development. One approach to limit spread arthropod‐borne diseases, such as yellow fever, West Nile virus malaria, reduce number potential vectors by interfering with this mosquito reproduction...
Mosquitoes are vectors of human pathogens that cause disease, such as malaria, yellow fever and dengue fever. Pathogen transmission occurs when the female mosquito takes a bloodmeal from host. The must bloodfeed for egg production, or oogenesis. An essential concentrated nutrient in blood meal is iron, which required optimal development viable offspring. Ferritin major iron‐storage transport protein expressed mosquitoes. increases following ovaries, midgut, fat body hemolymph. Insect...
In mammals, ferritin is a cytoplasmic iron storage protein made up of 24 heavy and light chain subunits that can store to 4500 atoms; as wells an acute phase secreted upon inflammation. Here we characterize the message expression profiles Aedes aegypti (yellow fever mosquito) (HCH) homologue (LCH) in response bacterial challenge. Mosquito experiments show increase midgut HCH LCH h post‐blood meal (PBM) decrease 72 PBM. Fat body ovary showed time‐dependent Heat‐killed bacteria (HKB) had...
Mosquitoes consume and absorb significant amount of iron from a blood meal. Ferritin is protein that used to store iron. Iron necessary for egg development in mosquitoes. We wanted examine the proteome ovaries Aedes aegypti (yellow fever mosquito) were fed an artificial meal with or without hemoglobin. The number proteins identified mosquitoes hemoglobin was 48 13 respectively. A previously uncharacterized glycosylated ferritin light chain homologue (G‐LCH) found rich diet. Primers designed...