A5085549448- Insect Resistance and Genetics
- Iron Metabolism and Disorders
- Invertebrate Immune Response Mechanisms
- Insect symbiosis and bacterial influences
- Mosquito-borne diseases and control
- Entomopathogenic Microorganisms in Pest Control
- Monoclonal and Polyclonal Antibodies Research
- Trace Elements in Health
- Protein purification and stability
- Insect Utilization and Effects
- Glycosylation and Glycoproteins Research
- Insect Pest Control Strategies
- Child Nutrition and Water Access
- Phytoplasmas and Hemiptera pathogens
- Escherichia coli research studies
- Plant Stress Responses and Tolerance
- Studies on Chitinases and Chitosanases
- Plant Physiology and Cultivation Studies
- Animal health and immunology
- Postharvest Quality and Shelf Life Management
- Plant Water Relations and Carbon Dynamics
- RNA regulation and disease
- RNA Research and Splicing
- Insect and Pesticide Research
- Plant biochemistry and biosynthesis
University of Arizona
2009-2020
Center for Advanced Legal Studies
2002-2012
Universidad de Sonora
2008
Centro de Investigación en Alimentación y Desarrollo
2008
Arizona Science Center
1995-2002
Stanford Synchrotron Radiation Lightsource
2002
ExxonMobil (United States)
2002
Nutrition Sciences (Belgium)
2000
South University
1992
Tsetse flies are the sole vectors of human African trypanosomiasis throughout sub-Saharan Africa. Both sexes adult tsetse feed exclusively on blood and contribute to disease transmission. Notable differences between other include obligate microbial symbioses, viviparous reproduction, lactation. Here, we describe sequence annotation 366-megabase Glossina morsitans genome. Analysis genome 12,308 predicted protein-encoding genes led multiple discoveries, including chromosomal integrations...
Abstract Ferritin, an iron storage protein, was isolated from larvae and pupae of Aedes aegypti grown in iron‐rich medium. Mosquito ferritin is a high molecular weight protein composed several different, relatively small, subunits. Subunits mass 24, 26, 28 kDa are equally abundant, while that 30 present only small amounts. The N‐terminal sequence the 24 26 subunits identical for first amino acids, subunit differs. Studies using antiserum raised against mixture showed were larvae, pupae,...
It has been shown that the seleno-bis (S-glutathionyl) arsinium ion, [(GS)2AsSe]-, is major arsenic and selenium excretory product in bile of rabbits treated with arsenite selenite [Gailer, J., Madden, S., Buttigieg, G. A., Denton, M. B., Younis, H. S. (2002) Appl. Organomet. Chem. 16, 72−75]. To investigate vivo interaction between other environmentally common oxy-anions mammals, we have intravenously injected different combinations oxo-anions (arsenite + selenate, arsenate selenite,...
In yellow fever mosquito cells (Aag2 clone), iron treatment induces a threefold increase in ferritin message ( fer mRNA) and protein (ferritin) by 16 h. These data contrast with work mammalian hepatocytes fibroblasts which mRNA levels do not change stimulation, but 50‐fold. Pretreatment of the Aag2 actinomycin D blocks induction reduces subunit synthesis, suggesting that synthesis is subjected to transcriptional control. A putative iron‐regulatory has also been identified cytoplasmic...
Zabrotes subfasciatus (Boheman) is the main pest of common beans (Phaselous vulgaris). Some wild legume seeds may contain lectins with insecticidal activities against this insect. The larval developments Z. on Olneya tesota (a desert legume) and artificial containing purified PF2 lectin were evaluated. susceptibility to proteolysis was assessed by incubation midgut extract at different times. binding glycoconjugates histochemistry. A reduced level oviposition a lack emergence adult beetles...
Abstract Mammalian ferritin subunit synthesis is controlled at the translational level by iron regulatory protein 1 (IRP1)/iron responsive element (IRE) interaction. Insect haemolymph messages have an IRE in 5′‐untranslated region (UTR). We shown that recombinant M. sexta IRP1 represses vitro translation of both heavy and light chain subunits from this species without altering transcription. Deletion either 5′‐UTR or mRNA abolishes repression. Our studies indicated control IRP/IRE...
Plants vary greatly in their ability to survive cold temperatures; some may withstand extreme freezing conditions, while others will be irreversibly injured at temperatures above freezing. The maximum tolerance of plants is induced response environmental signals. Temperate woody trees need acclimate the winter. Trees have evolved a complex dynamic process controlling development dormancy and hardiness that synchronize accurately onset termination winter metabolism. Only recently has been...
We have isolated and sequenced a genomic clone encoding the 24- 26-kDa ferritin subunits in mosquito Aedes aegypti (Rockefeller strain). The A. gene differs from other known genes that it possesses an additional intron unusually large second intron. is located within 5' untranslated region, between CAP site start codon. contains numerous putative transposable elements. In addition, unlike human rat genes, single copy gene, at 88.3% FLpter on q-arm of chromosome 1. Primer extension analysis...
Amylases are an important family of enzymes involved in insect carbohydrate metabolism that required for the survival larvae. For this reason, from starch-dependent insects targets insecticidal control. PF2 (Olneya tesota) is a lectin toxic to Zabrotes subfasciatus (Coleoptera: Bruchidae) In study, we evaluated recognition α-amylases Z. midgut and effect on α-amylase activity. caused decrease total amylase activity vitro. Subsequently, several isoforms were isolated tissues using ion...
Zabrotes subfasciatus (Boheman) is the main pest of common beans ( Phaselous vulgaris ). Wild legume seeds from Olneya tesota contain a lectin, PF2, that shows insecticidal activities against this insect. The binding PF2 to midgut glycoproteins 20-day-old larvae was evaluated using affinity chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis proteins retained on revealed several putative glycoproteins, ranging in mass 17 97 kDa. Subsequent protein...
Recently, glyco-therapy is proposed to prevent the interaction of bacterial lectins with host ligands (glycoconjugates). This represents first step in infection. Neoglycans referred as PSA-Lac (PSA-Glu (β1-4) Gal) were obtained by conjugation porcine serum albumin (PSA) lactose at 80 °C, 100 °C and 120 ºC. Characterization studies products showed that PSA could contain 1, 38 or 41 added lactoses, depending on reaction temperature. These neoglycans approximately 10 times more glycated than...