Milk, due to its diversity in terms of nutritional content, is an important element the human diet, as well a good medium for development bacteria. The genus Bacillus contains ubiquitous aerobic, rod-shaped, endospore-producing gram-positive Representatives cereus group and subtilis contribute shortening shelf life milk dairy products by degrading components additives. They also produce number heat-stable toxins can cause ailments, mainly digestive system. aim this research was identify sp....

The capacity for biofilm formation is one of the crucial factors staphylococcal virulence. occurrence biofilm-forming staphylococci in raw milk may result disturbances technological processes dairy factories as well contamination finished food products. Therefore, this study aimed to determine prevalence and characteristics samples explore genetic background associated with those isolates. material subjected testing included 30 cow’s acquired from farms central part Poland. A total 54...

Bacterial biofilm is one of the major hazards facing food industry. Biofilm-forming ability most important virulence properties enterococci. The genus Enterococcus includes pathogenic, spoilage, and pro-technological bacteria. presence enterococci in milk dairy products usually associated with inadequate hygiene practices. study examined isolates' capacity for formation identification genetic determinants its among 85 strains isolated from raw (n = 49) soft-ripened cheeses made unpasteurized...

Cheeses produced from unpasteurized milk by traditional production methods may contain many groups of microorganisms, including Staphylococcus aureus. The aim this study was to determine the occurrence S. aureus in artisanal cheese chain milk. We investigated prevalence strains isolated various stages farms northeastern and southern parts Poland characterized them. Characterization included antimicrobial susceptibility microbroth dilution biofilm formation vitro assay. Among all strains,...

Background:Staphylococcus aureus is one of the most prevalent pathogens causing mastitis in dairy animals and represents a serious issue public health concern due to its resistance against multiple antimicrobials. Objectives: This study assessed 101 S. isolates obtained from quarter milk with subclinical Ragusa area (Sicily, Italy). Methods: Antibiotic nine antibiotics was evaluated using Kirby-Bauer method, Minimum Inhibitory Concentration (MIC) values were measured for oxacillin (OXA)...

Antimicrobial resistance is spreading rapidly throughout the world. The food chain can be one of routes transmission for microorganisms containing drug-resistance genes and thus serve as a channel their transmission. Environmental stress methods preventing spread trigger adaptive responses in bacterial cells. aim present study was to determine effect induced by sub-inhibitory concentrations (SICs) cadmium chloride benzalkonium on antibiotic expression selected virulence factors...

Although coagulase-positive staphylococci are considered to be the main factor responsible for food poisoning, an increasing role coagulase-negative in production of enterotoxins has been observed recent years. This study was conducted assess occurrence genes staphylococcal (SE), enterotoxin-like toxins (SEI) and toxic shock syndrome toxin-1 (TSST-1) (CoNS) isolated from ready-to-eat bars restaurants. One hundred eighteen CoNS strains were tested using polymerase chain reaction (PCR) five...

The present study aimed to characterize and assess the diversity of CoNS strains as potential vectors for spread resistance antimicrobial agents from RTE foods served in bars restaurants. Eighty-five strains, obtained 198 food samples, were investigated. Sixty-seven isolates (78.8%) resistant at least one antibiotic tested, 37 (43.5%) multidrug (MDR-CoNS). Moreover, contained genes conferring antibiotics critically important medicine, i.e., β—lactams [mecA (29.4%); blaZ (84.7%)],...

Photodynamic inactivation (PDI) is a highly effective treatment that can eliminate harmful microorganisms in variety of settings. This study explored the efficacy curcumin-rich extract, Curcuma L., (Cur)- and essential oil component, trans-cinnamaldehyde, (Ca)-mediated PDI against Listeria monocytogenes ATCC 15313 (Lm) including planktonic cells established biofilms on silicone rubber (Si), polytetrafluoroethylene (PTFE), stainless steel 316 (SS), polyethylene terephthalate (PET). Applying...

The aim of this study was to evaluate the possibility horizontal transfer genes encoding resistance aminoglycosides (aac(6')-Ie-aph(2″)-Ia, aph(2″)-Ib, aph(2″)-Ic, aph(2″)-Id, ant(4')-Ia and ant(6')-Ia), tetracyclines (tetM, tetL, tetK, tetO tetW), macrolides (ermA, ermB, ermC, msrC, mefAB) in Enterococcus strains isolated from ready-to-eat dishes purchased bars restaurants Olsztyn, Poland.It found that 74% tested were able conjugal at least one antibiotic genes. Transfer observed with a...

Sous-vide is a cooking technique which considered safe for food preparation, however, the recommended temperatures and times preparing seafood fish may not be sufficient to ensure safety, additionally, thermal stress cause changes in virulence resistance. The objective of present investigation was determine reduction Listeria monocytogenes isolated from after sous-vide preparation. For this purpose flow cytometry used. impact during preparation fish-based dish using on potential resistance...

Staphylococcus aureus is one of the most important foodborne pathogens. S. has capability to produce a variety toxins, including staphylococcal enterotoxins (SEs). The aim this study was evaluate survival rate cells and analyze gene expression after exposure osmotic stress acidic/alkaline stress. To determine rates, traditional plate counting method flow cytometry were used. levels enterotoxin genes performed by quantitative reverse transcription PCR (RT-qPCR). Expression changes differed...

This study aimed to genotypic and phenotypic analyses of the enterotoxigenic potential Staphylococcus spp. isolated from raw milk cheeses. The presence genes encoding staphylococcal enterotoxins (SEs), including classical (sea-see), non-classical (seg-seu), exfoliative toxins (eta-etd) toxic shock syndrome toxin-1 (tst-1) were investigated. Isolates positive for enterotoxin then tested by SET-RPLA methods toxin expression. Out 75 (19 aureus 56 CoNS) isolates (49/65.3%) cheese samples...

This paper provides a snapshot on the pathogenic traits within CoNS isolated from ready-to-eat (RTE) food. Eighty-five strains were subjected to biofilm and slime production, as well biofilm-associated genes (icaA, icaD, icaB, icaC, eno, bap, bhp, aap, fbe, embP atlE), insertion sequence elements IS256 IS257 hemolytic genes. The results showed that most prevalent determinants responsible for primary adherence eno (57.6%) aap (56.5%) icaADBC operon was detected in 45.9% of tested correlated...

Staphylococcus aureus is one of the major foodborne pathogens contaminating milk and products. Isolates recovered from different stages in cheese production chain food processing environment (n = 16) Poland were characterised using whole genome-sequencing bioinformatic analyses. Most strains belonged to multi-locus sequence type (MLST) ST109 (6/37.5%) ST130 (3/18.8%). Spa-typing S. isolates revealed six spa types (t693, t91, t3218, t571, t359, t1109, t2). At least gene belonging groups...

In the present study, 30 L. monocytogenes strains isolated from bivalves purchased in Poland were characterized by whole genome sequencing (WGS). The Core Genome Multilocus Sequence Typing (cgMLST) analysis revealed that most frequent serogroups IIa; sequence types (ST) ST101, ST21 and ST325; clonal complexe (CC) CC101. Despite differential genotypic subtypes, had similar antimicrobial resistance profiles. Most genetic determinants of to many groups antibiotics; aminoglycosides,...

In the present study, 30 L. monocytogenes strains isolated from bivalves were characterized by whole genome sequencing (WGS). The Core Genome Multilocus Sequence Typing (cgMLST) analysis revealed that most frequent serogroups IIa; sequence types (ST) ST101, ST21 and ST325; clonal complexe (CC) CC101. Despite differential genotypic subtypes, had similar antimicrobial resistance profiles. Most genetic determinants of to many groups antibiotics; aminoglycosides, fluoroquinolones, lincosamides,...