Enterobacterales is a prevalent order, which inhabits variety of environments including food. Due to the high similarities between pathogenic and non-pathogenic species, their identification might be difficult laborious, therefore there need for rapid precise identification. The aim this study was compare effectiveness available methods identifying order strains isolated from fresh fish shrimps (n = 62). following were used in study: biochemical, sequencing using matrix-assisted laser...

This study analyzed antibiotic resistance and the occurrence of genes in strains from starter protective cultures. The largest number obtained belonged to genus Lactobacillus (n = 26; 44.9%), followed by Lactococcus 17; 29.3%), Pediococcus 8; 13.8%), Streptococcus 4; 6.9%), Staphylococcus 2; 3.4%) Leuconostoc 1; 1.7%). Among all strains, highest Minimum Inhibitory Concentrations (MICs) were noted for tetracycline (max. >256 μg/ml), kanamycin 48 μg/ml) chloramphenicol 32 μg/ml). most common...

The aim of study was to determine the occurrence virulence factors and virulence-related genes among enterococci isolated from food animal origin effects osmotic high pressure stress on expression genes. number 78 isolates were analyzed. None them showed a strong ability form biofilm, 38.5% (n = 30) had slime production ability, 41% 32) gelatinase activity, γ -type hemolysis observed in 55% isolates, α-type 45%. All carried 1-13 most common gelE (85.9%), sprE (78.2%) asa1 (75.6%). There also...

This study analyzed the effect of food-related stresses on expression antibiotic resistance starter and protective strains gene transfer frequency. After exposure to high-pressure processing, acidic osmotic stress, genes encoding aminoglycosides (aac(6')Ie-aph(2″)Ia aph(3')-IIIa) and/or tetracyclines (tetM) increased. cold a decrease in level all tested was observed. The results obtained analysis correlated with phenotype patterns. stresses, significant increase frequency each To best...

The aim of this study was to evaluate the possibility horizontal transfer genes encoding resistance aminoglycosides (aac(6')-Ie-aph(2″)-Ia, aph(2″)-Ib, aph(2″)-Ic, aph(2″)-Id, ant(4')-Ia and ant(6')-Ia), tetracyclines (tetM, tetL, tetK, tetO tetW), macrolides (ermA, ermB, ermC, msrC, mefAB) in Enterococcus strains isolated from ready-to-eat dishes purchased bars restaurants Olsztyn, Poland.It found that 74% tested were able conjugal at least one antibiotic genes. Transfer observed with a...

Enterococci are important opportunistic pathogens with the capacity to acquire and spread antibiotic resistance. At present, linezolid-resistant enterococci (LRE) pose a great challenge. Linezolid is considered as last resort in treatment of enterococcal infections, so it monitor occurrence LRE various environments. The aim this study was define genetic mechanisms linezolid resistance (E. faecalis, E. faecium, hirae, casseliflavus) isolated from foods animal origin (n = 104). (LR) shown by...

This study analyzed the effect of high-pressure processing on changes in resistance phenotype and expression antibiotic genes among strains from commercial starter cultures. After exposure to high pressure encoding aminoglycosides (aac(6')Ie-aph(2″)Ia aph(3')-IIIa) decreased tetracyclines (tetM tetW), ampicillin (blaZ) chloramphenicol (cat) increased. Expression differed depending variant chosen. The results obtained gene analysis correlated with patterns. To best authors' knowledge, this is...

The aim of this study was phenotypic and genotypic characterization antibiotic-resistant food-borne Enterobacterales. largest number isolates identified as Enterobacter cloacae (42.4%) followed by Escherichia coli (9.8%), Proteus mirabilis, Salmonella enterica, penneri, Citrobacter freundii (7.6% each), braakii (6.6%), Klebsiella pneumoniae oxytoca (5.4% each). More than half (52.2%) were resistant to at least one antibiotic. majority amoxicillin-clavulanate (28.3%) ampicillin (19.5%)....

High-pressure processing is one of the most promising novel food preservation methods that increasingly used in industry. Its biggest advantage it a nonthermal method ensures microbiological safety product while maintaining other features, including nutritional value. If products made with starter cultures are subjected to high-pressure treatment, process parameters should be selected so as not eliminate all microorganisms product. The aim study was investigate if carrying antibiotic...

This study analyzed the effect of high-pressure processing on changes in resistance phenotype and expression antibiotic genes among strains from commercial starter cultures. After exposure to high pressure encoding aminoglycosides ( aac(6’)Ie-aph(2’’)Ia aph(3’)-IIIa ) decreased tetracyclines tet M W), ampicillin bla Z) chloramphenicol cat increased. Expression differed depending variant chosen. The results obtained gene analysis correlated with patterns. To best authors’ knowledge, this is...

This study analyzed the effect of food-related stresses on expression antibiotic resistance starter and protective strains gene transfer frequency. After exposure to high-pressure processing, acidic osmotic stress, genes encoding aminoglycosides ( aac(6’)Ie-aph(2’’)Ia aph(3’)-IIIa ) and/or tetracyclines tet M) increased. cold a decrease in level all tested was observed. The results obtained analysis correlated with phenotype patterns. stresses, significant increase frequency each To best...