A5030645615- Birth, Development, and Health
- Catalytic Processes in Materials Science
- Neonatal Respiratory Health Research
- Catalysis and Oxidation Reactions
- CRISPR and Genetic Engineering
- DNA Repair Mechanisms
- RNA Research and Splicing
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Industrial Gas Emission Control
- Pregnancy and preeclampsia studies
- RNA modifications and cancer
- Diet and metabolism studies
- Genetics and Neurodevelopmental Disorders
- Adrenal Hormones and Disorders
- interferon and immune responses
- Healthcare and Venom Research
- Child Nutrition and Water Access
- Congenital Anomalies and Fetal Surgery
- Genomics and Chromatin Dynamics
- Ubiquitin and proteasome pathways
- Folate and B Vitamins Research
- Pituitary Gland Disorders and Treatments
- Catalysis and Hydrodesulfurization Studies
Shanghai University
2024
Centre Hospitalier de l’Université de Montréal
2017-2024
Lunenfeld-Tanenbaum Research Institute
2023
Mount Sinai Hospital
2023
Zhejiang University
2015
Children's Hospital of Zhejiang University
2015
Abstract Background Neural tube defects (NTDs) are caused by genetic and environmental factors. ARMC5 is part of a novel ubiquitin ligase specific for POLR2A, the largest subunit RNA polymerase II (Pol II). Results We find that knockout mice have increased incidence NTDs, such as spina bifida exencephaly. Surprisingly, absence causes accumulation not only POLR2A but also most other 11 Pol subunits, indicating degradation whole complex compromised. The enlarged pool does lead to generalized...
Abstract Armadillo repeat containing 5 (ARMC5) is a cytosolic protein with no enzymatic activities. Little known about its function and mechanisms of action, except that gene mutations are associated risks primary macronodular adrenal gland hyperplasia. Here we map Armc5 expression by in situ hybridization, generate knockout mice, which small body size. mice have compromised T-cell proliferation differentiation into Th1 Th17 cells, increased apoptosis, reduced severity experimental...
Abstract ARMC5 is implicated in several pathological conditions, but its function remains unknown. We have previously identified CUL3 and RPB1 (the largest subunit of RNA polymerase II (Pol II) as potential ARMC5-interacting proteins. Here, we show that ARMC5, RBX1 form an active E3 ligase complex specific for RPB1. CUL3, formed Armc5 deletion caused a significant reduction ubiquitination increase accumulation RPB1, hence enlarged Pol pool normal tissues organs. The compromised degradation...
To maintain genome integrity, cells must accurately duplicate their and repair DNA lesions when they occur. uncover genes that suppress damage in human cells, we undertook flow-cytometry-based CRISPR-Cas9 screens monitored damage. We identified 160 whose mutation caused spontaneous damage, a list enriched essential genes, highlighting the importance of genomic integrity for cellular fitness. also 227 replication-perturbed cells. Among characterized, discovered deoxyribose-phosphate aldolase...
Early postnatal life is considered as a critical time window for the determination of long-term metabolic states and organ functions. Extrauterine growth restriction (EUGR) causes development adult-onset chronic diseases, including pulmonary hypertension. However, effects nutritional disadvantages during early period on vascular consequences in later are not fully understood. Our study was designed to test whether epigenetics dysregulation mediates cellular memory this event.To hypothesis,...
Extrauterine growth restriction (EUGR) plays an important role in the developmental origin of adult cardiovascular diseases. In EUGR rat model, we reported elevated pulmonary arterial pressure adults and genome-wide epigenetic modifications vascular endothelial cells (PVECs). However, underlying mechanism early nutritional insult that results consequences later life remains unclear. A model was used to investigate physiological structural effect on vasculature by evaluating right ventricular...
Objective: Early postnatal life is considered as a critical time window for determination of long-term metabolic states and organ functions. Extrauterine growth restriction (EUGR) causes the development adult onset chronic diseases, including pulmonary hypertension (PH). However, mechanisms involved possibilities transgenerational transmission on vascular consequences in later are still unclear. Epigenetic information can be inherited represents plausible carrier environmental information....
Searchable abstracts of presentations at key conferences in endocrinology ISSN 1470-3947 (print) | 1479-6848 (online)