The purposes of this study were to 1) examine the immune and oxidative stress responses following high-intensity interval training (HIIT); 2) determine changes in antioxidant enzyme gene expression activity lymphocytes HIIT; 3) assess pre-HIIT, 3-h post-HIIT, 24-h post-HIIT lymphocyte cell viability hydrogen peroxide exposure vitro. Eight recreationally active males completed three identical HIIT protocols. Blood samples obtained at preexercise, immediately postexercise, 3 h 24 postexercise....

In the isolated rat kidney, vasoconstrictor response elicited by periarterial nerve stimulation at low frequencies (2 Hz) is resistant to alpha adrenergic receptor blockade. It has been proposed that in some blood vessels ATP coreleased with norepinephrine during activate P2-purinergic receptors and responsible for component of To assess contribution a purinergic transmitter vasoconstriction Tyrodes-perfused fractional overflow [3H]norepinephrine responses renal were examined after blockade...

Administration of the 1-deamino-arginine8 vasopressin caused a decrease in right, but not left, atrial levels atriopeptin (AP) chloral hydrate-anesthetized rats. The amount exogenous AP required to match vasopressin-stimulated plasma was equivalent content right atrium, suggesting that resynthesis did occur within 60 min after stimulation. correlation between increased and decreased also observed bilaterally nephrectomized rats, kidney is major site degradation vivo. This finding confirmed...

A manganese(II) complex with a ligand containing an oxidizable quinol group serves as turn-on sensor for H2O2. Upon oxidation, the relaxivity of in buffered water increases by 0.8 mM(-1) s(-1), providing signal that can be detected and quantified magnetic resonance imaging. The also potent antioxidant, suggesting this related complexes have potential to concurrently visualize alleviate oxidative stress.

Ni( ii ) complexes with quinol-containing ligands are efficient catalysts for the degradation of superoxide despite relying on redox couples that organic, rather than metal-centered. The activities diminish but remain high in phosphate buffer.

α 2 ‐Adrenergic receptor agonists are widely used in veterinary medicine as sedative/hypnotic agents. Four pharmacological subtypes of the ‐adrenergic (A, B, C and D) have been identified based primarily on differences affinity for several drugs. The purpose this study was to examine affinities sedative agents, xylazine, detomidine medetomidine at four subtypes. Saturation inhibition binding curves were performed membranes tissues containing only one subtype a receptor. K D radioligand, [ 3...

The overproduction of reactive oxygen species has been linked to a wide array health disorders. ability noninvasively monitor oxidative stress in vivo could provide substantial insight into the progression these conditions and, turn, facilitate development better diagnosis and treatment options. A mononuclear Mn(II) complex with redox-active ligand N,N'-bis(2,5-dihydroxybenzyl)-N,N'-bis(2-pyridinylmethyl)-1,2-ethanediamine (H4qtp2) was made characterized. previously prepared containing...

Abstract Serum, plasma and tissue expression of vascular endothelial growth factor (VEGF) was measured in 20 dogs previously diagnosed histologically with oral melanoma. The concentrations VEGF serum were significantly higher melanoma than a control population ( P ≤ 0.002). Concentrations the highly correlated r = 0.867). Ninety‐five per cent tissues expressed VEGF. Two staining patterns detected: diffuse granular cytoplasmic staining. High blood to shorter survival time receiving definitive...

The purpose of this study was to determine lactate transport kinetics in single isolated rat ventricular cardiac myocytes after 1) 8 wk myocardial volume overload (MVO) and 2) congestive heart failure (CHF). Twenty male Sprague-Dawley rats were assigned one four groups: hypertrophy (MH), MH sham (MHS), CHF, or CHF (CHFS). A chronic MVO induced the groups by an infrarenal arteriovenous fistula. Postdeath lung weights significantly greater ( P < 0.05) for compared with controls. Isolated...

Abstract Objective: To determine the effect of fenoldopam infusion on urine output, sodium excretion, creatinine clearance, and indirect blood pressure in healthy cats. Design: Prospective study. Setting: Veterinary medical teaching hospital. Animals: Eight purpose‐bred cats, 2–4 years old. Interventions: None. Measurements: Urine output was measured hourly for 12 hours before after administration. Sodium modified fractional excretion were following specific gravity, central venous pressure,...

Scutellaria lateriflora (American skullcap), a native plant of North America, has been used by Americans and Europeans as nerve tonic for more than 200 years. In vivo studies have shown anxiolytic activity ofS. in animals humans. However, the neuroprotective mechanisms are not fully understood. Oxidative stress plays vital role neurodegenerative neuropsychiatric diseases such anxiety, Alzheimer's disease, depression, Parkinson's disease. Bioactive compounds present various medicinal plants...

The melanocortin receptors (MCRs 1-5) are G protein coupled-receptors (GPCRs) that regulate food intake, inflammation, skin pigmentation, sexual function and steroidogenesis. Their peptide ligands, the melanocortins, α-, β- γ-melanocyte-stimulating hormone adrenocorticotropic (ACTH) all of which secreted from anterior pituitary gland under hypothalamic control. MC2R binds ACTH but has no affinity for other melanocortins is, thereby, pharmacologically different MCRs bind those ligands....

Atrazine is one of the most commonly used pre-emergence and early post-emergence herbicides in world. We have shown previously that atrazine does not directly stimulate pituitary or adrenal to trigger hormone release but acts centrally activate a stress-like activation hypothalamic-pituitary-adrenal axis. In doing so, treatment has been cause morphology changes characteristic repeated stress. this study, adrenals from treated stressed animals were compared after 4 days restraint Both...

In the current work, we demonstrate ligand design concepts that significantly improve superoxide dismutase (SOD) activity of a zinc complex; catalysis is enhanced when two quinol groups are present in polydentate ligand. We investigate mechanism through which quinols influence and determine impact entirely removing chelating group from original hexadentate Our results suggest SOD mimicry with these compounds requires coordinates Zn(II) strongly both its oxidized reduced forms proceeds...

Whole-cell electrophysiological studies suggest that sympathetic nerve alpha-2 adrenergic receptors are coupled to voltage-dependent N-type calcium channels through the Gi family of proteins inhibit neurotransmitter release. Because most terminals too small for direct recordings, aim this study was examine relationship between receptor-mediated inhibition norepinephrine release and rise in cytosolic neurites from cultured neurons. In rat superior cervical ganglion neurons, receptor agonists,...

The objectives of this study were 1) to determine whether ANG II-induced myocardial damage (ANG Dam) is mediated via the β 1 -adrenergic receptor, 2) elucidate adrenal medulla or cardiac sympathetic neuron catecholamines are responsible for Dam, and 3) lack after 3 days elevated II levels due -receptor downregulation. To end, was administered rats that treated with a β-receptor blocker, medullectomy and/or sympathectomy, 8 days. caused both myocyte necrosis coronary vascular but not...

The presence of dopamine (DA) receptors in feline kidneys is a matter contention. Radioligand binding and Western blotting studies were employed to determine whether DA are present kidneys. pharmacologic profile the selective D1-receptor antagonist [3H]-SCH 23390 was studied renal cortical membrane preparations from cats by conducting saturation isotherm competitive experiments. bound membranes manner consistent with labeling D1-like receptor. revealed single site or D1 receptor cortex Kd =...

Abstract Hemophilia A is an X‐chromosome‐linked disorder caused by a deficiency in factor VIII ( FVIII ). Although foals have been diagnosed with hemophilia based on activity, causative gene mutations not identified. The genomic DNA and cDNA encoding of Tennesee Walking Horse colt affected the his dam normal unrelated horse were analyzed no splice site or coding sequence abnormalities identified any horses. Polymerase chain reactions (PCR) then performed hepatic from horse, product was...

Cardiac β-adrenergic receptors are the primary driving force for enhancement of contractility in response to sympathetic stimulation. Angiotensin II influences cardiac function by modulating activity and activating angiotensin receptors. The aim this study was determine whether activation modulates responsiveness heart receptor activation. Male Sprague-Dawley rats were anesthetized hearts isolated perfused with oxygenated Krebs-Henseleit buffer (KHB). Coronary artery perfusion pressure, left...