A5090600647- HIV Research and Treatment
- Gut microbiota and health
- HIV/AIDS Research and Interventions
- Clostridium difficile and Clostridium perfringens research
- HIV/AIDS drug development and treatment
- Ubiquitin and proteasome pathways
- DNA Repair Mechanisms
- HIV/AIDS oral health manifestations
- Pneumocystis jirovecii pneumonia detection and treatment
- Cancer-related Molecular Pathways
- Cannabis and Cannabinoid Research
- Immune Cell Function and Interaction
- Dermatology and Skin Diseases
- Malaria Research and Control
- Plant and soil sciences
- Reproductive tract infections research
University of Washington
2016-2022
Oregon National Primate Research Center
2017-2019
Cannabis is a widely used drug in the United States, and frequency of cannabis use human immunodeficiency virus (HIV)-infected population disproportionately high. Previous macaque studies suggest that may have an impact on plasma viral load; however, relationship between HIV-associated systemic inflammation immune activation has not been well defined.The peripheral cell frequency, activation, function was assessed 198 HIV-infected, antiretroviral-treated individuals by flow cytometry....
Gastrointestinal (GI) mucosal dysfunction predicts and likely contributes to non-infectious comorbidities mortality in HIV infection persists despite antiretroviral therapy. However, the mechanisms underlying this remain incompletely understood. Neutrophils are important for containment of pathogens but can also contribute tissue damage due their release reactive oxygen species other potentially harmful effector molecules. Here we used a flow cytometry approach investigate increased...
Simian-human immunodeficiency viruses (SHIVs) have been utilized to test vaccine efficacy and characterize mechanisms of viral transmission pathogenesis. However, the majority SHIVs currently available significant limitations in that they were developed using sequences from chronically HIV-infected individuals or uncommon HIV subtypes optimized for macaque model by serially passaging engineered virus vitro vivo Recently, a newly SHIV, SHIV.C.CH505.375H.dCT (SHIV.CH505), which incorporates...
The tumor-suppressor protein BRCA1 works with BARD1 to catalyze the transfer of ubiquitin onto substrates. N-terminal regions and that contain their RING domains are responsible for dimerization ligase activity. This activity is a common feature among hundreds human domain-containing proteins. bind activate E2 ubiquitin-conjugating enzymes promote We show identity residues at specific positions in domain can tune levels up or down. report substitutions create structurally intact BRCA1/BARD1...
An effective vaccine to prevent HIV transmission has not yet been achieved. Modulation of the microbiome via probiotic therapy suggested result in enhanced mucosal immunity. Here, we evaluated whether could improve immunogenicity and protective efficacy SIV/HIV vaccination. Rhesus macaques were co-immunized with an DNA particle-mediated epidermal delivery protein administered intramuscularly Adjuplex™ adjuvant, while receiving daily oral Visbiome® probiotics. Probiotic alone led reduced...
In Sub-Saharan Africa, young women 15–24 years of age account for nearly 30% all new HIV infections, however, biological and epidemiological factors underlying this disproportionate infection rate are unclear. study, we assessed contributors SIV/HIV susceptibility in the female genital tract (FGT) using adolescent (n = 9) adult 10) pigtail macaques (PTMs) with weekly low-dose intravaginal challenges SIV. Immunological variables were captured vaginal tissue PTMs by flow cytometry cytokine...
Abstract While functions of the gastrointestinal (GI) microbiome include maintenance immune homeostasis and protection against infectious disease, its role in determining disease severity during Plasmodium infection has been limited to mouse models observational human cohorts. Here, we performed controlled both humans rhesus macaques (RMs) experimentally determine impact GI composition on progression. Through analysis serially collected samples, identified a high-risk microbial signature...