Abstract Alginates are polysaccharides with many industrial and medical uses, from food additives to encapsulation agents in the emerging transplantation technologies. Alginate is composed of variable proportions β‐D‐mannuronic acid α‐L‐guluronic linked by 1–4 glycosidic bonds. Traditionally, commercial alginate has been produced farmed brown seaweeds, but this suffers heterogeneity composition quality partly due environmental variation. Two bacterial genera, Pseudomonas Azotobacter, also...

Quorum sensing (QS) is the process by which bacteria communicate with each other through small signaling molecules, such as N-acylhomoserine lactones (AHLs). Also, certain have ability to degrade AHL molecules a referred quorum quenching (QQ); therefore, QQ can be used control bacterial infections and biofilm formation. In this study, we aimed identify new species of activities. To achieve this, Red Sea sediments were collected either in close vicinity grass or from area no vegetation. From...

This work describes the chemical composition of extracellular polymeric substances (EPS) produced by three bacteria (RO1, RO2, and RO3) isolated from a biofouled reverse osmosis (RO) membrane. We pure cultures bacterial strains 7-year-old RO module that was used in full-scale seawater treatment plant. All showed similar growth rates, biofilm formation, quantities proteins polysaccharides. The gel permeation chromatography EPS all has high molecular weight; however, RO1 RO3 highest weight....

ABSTRACT Pseudomonas aeruginosa is an opportunistic pathogen of particular significance to cystic fibrosis patients. This bacterium produces the exopolysaccharide alginate, which indicator poor prognosis for these The proteins required alginate polymerization and secretion are encoded by genes organized in a single operon; however, existence internal promoters has been reported. It proposed that form multiprotein complex extends from inner outer membrane. Here, experimental evidence...

Quorum quenching (QQ) is the enzymatic degradation of molecules used by bacteria for synchronizing their behavior within communities. QQ has attracted wide attention due to its potential inhibit biofilm formation and suppress production virulence factors. Through capacity limit biofouling infections, applications in water treatment, aquaculture, healthcare. Several different enzymes have been described; however, they often lack high stability catalytic efficiency required industrial...

This study describes a novel integration of aerobic granular sludge (AGS) with gravity-driven membrane (GDM) system at pilot scale treatment capacity approximately 150 L per day to treat raw domestic wastewater. The performance and energy consumption the AGS-GDM were compared neighboring full-scale bioreactor (AeMBR), treating same wastewater about 4000(±500) m3 day. demonstrated superior nutrient (nitrogen phosphorus) removal as AeMBR. GDM unit was continuously supplied AGS-treated...

An alarming increase in antibiotic-resistant bacterial strains is driving clinical demand for new antibacterial agents. One of the oldest antimicrobial agents elementary silver (Ag), which has been used thousands years. Even today, Ag medical purposes such as treating burns, wounds, and microbial infections. In consideration effectiveness Ag, present researchers generated effective antibacterial/antibiofilm by combining with biocompatible ultrashort peptide compounds. The innovative...

Abstract This study investigated the potential of ethanolic garlic extract-loaded chitosan hydrogel film for burn wound healing in an animal model. The extract was prepared by macerating fresh ground cloves ethanol 24 h, followed filtration and concentration using a rotary evaporator. Hydrogels were then casting solution with added at varying concentrations optimization and, following drying, subjected to various characterization tests, including moisture adsorption (MA), water vapor...

Algal organic matter (AOM), produced by marine phytoplankton during bloom periods, may adversely affect the performance of membrane processes in seawater desalination.The polysaccharide fraction AOM has been related to (bio)fouling micro-filtration and ultrafiltration, reverse osmosis membranes.However, so far, chemical structure polysaccharides released bloom-forming algae is not well understood.In this study, dissolved three algal species (Chaetoceros affinis, Nitzschia epithemoides...

ABSTRACT The ubiquitous opportunistic human pathogen Pseudomonas aeruginosa secretes a viscous extracellular polysaccharide, called alginate, as virulence factor during chronic infection of patients with cystic fibrosis. In the present study, it was demonstrated that outer membrane protein AlgE is required for production alginate in P. . An isogenic marker-free algE deletion mutant constructed. This strain incapable producing but did secrete degradation products, indicating polymerization...

ABSTRACT AlgE is a monomeric 18-stranded β-barrel protein required for secretion of the extracellular polysaccharide alginate in Pseudomonas aeruginosa . To assess molecular mechanism secretion, was subjected to site-specific and FLAG epitope insertion mutagenesis. Except β-strands 6 10, insertions into transmembrane abolished localization outer membrane. Interestingly, an β-strand 10 produced only detectable membranes isolated from cells grown on solid media. The deletion nine C-terminal...

The Tampa Bay Water seawater reverse osmosis (SWRO) facility is the first large-capacity desalination plant in United States. feedwater source for an estuarine system that biologically very productive and contains naturally-occurring high concentrations of algae, marine bacteria, total organic carbon (mostly dissolved), transparent exopolymer particles (TEP), biopolymer fraction natural matter, phosphate. high-organic composition places stress on conventional sand pretreatment utilized at...

Fouling caused by organic matter and bacteria remains a significant challenge for the membrane-based desalination industry. decreases permeate quality membrane performance also increases energy demands. Here, we quantified amount of at several stages along water-treatment train an integrated ultrafiltration–nanofiltration seawater treatment pilot plant. We matter, in terms Total Organic Carbon (TOC) Assimilable (AOC), evaluated its composition using Liquid Chromatography Detection (LC-OCD)....

The study was undertaken to enhance the production of b-galactosidase using five organic nitrogen sources with wheat bran as a substrate under solid state fermentation. microbial source Aspergillus niger and its DG-resistant mutant that were grown in medium initial pH 5.5 250 ml flasks at 30°C for 144 h sample harvested after every 24 analysed consumption, cell mass formation enzyme production. All sources, ammonium sulphate, corn steep liquor, diammonium phosphate, fish meal urea showed...

Objectives: The objective of this study was to determine the 30 years (1990 2019) trends rheumatic heart diseases (RHD) in terms estimated prevalence, deaths, and disability-adjusted life (DALYs) for Pakistan. Methodology: This ecological consisted an analysis data extracted from Global Burden Disease Study, which (from 1990 trend RHD Pakistan DALYs, deaths number cased age-standardized rate (ASR) per 100,000 population. Results: prevalence has shown a 134.1% increase 705,795...