A5081004181- Heat shock proteins research
- Sperm and Testicular Function
- S100 Proteins and Annexins
- Viral Infectious Diseases and Gene Expression in Insects
- thermodynamics and calorimetric analyses
- Endoplasmic Reticulum Stress and Disease
- Thyroid Cancer Diagnosis and Treatment
- Natural product bioactivities and synthesis
- Reproductive Biology and Fertility
- Genetic factors in colorectal cancer
- Genetics, Aging, and Longevity in Model Organisms
- Cancer-related gene regulation
- Cancer-related Molecular Pathways
- Estrogen and related hormone effects
- Viral gastroenteritis research and epidemiology
- Carcinogens and Genotoxicity Assessment
- Genomics and Chromatin Dynamics
- Renal and related cancers
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- interferon and immune responses
- DNA Repair Mechanisms
The Maria Sklodowska-Curie National Research Institute of Oncology
2010-2020
Background The molecular mechanisms driving the papillary thyroid carcinoma (PTC) are still poorly understood. most frequent genetic alteration in PTC is BRAFV600E mutation–its impact may extend even beyond genomic profile and influence tumor characteristics clinical behavior. Methods In order to identify BRAF-dependent signature of early carcinogenesis PTC, a transgenic mouse model with BRAFV600E-induced was developed. Mice samples were used microarray analysis data referred human dataset....
Heat Shock Factor 1 (HSF1) is the primary transcription factor responsible for response to cellular stress, while HSF2 becomes activated during development and differentiation, including spermatogenesis. Although both factors are indispensable proper spermatogenesis, activation of HSF1 by heat shock initiates apoptosis spermatogenic cells leading infertility males. To characterize mechanisms assisting such induced we studied how cooperate response. For this purpose used chromatin...
Heat shock transcription factor 1 (HSF1), the major regulator of stress response, is frequently activated in cancer and has an apparent role malignant transformation. Here we analyzed influence over-expression a constitutively active transcriptionally-competent HSF1 mutant form on phenotypes mouse human melanoma cells. We observed that expression supported anchorage-independent growth vitro, metastatic spread animal model vivo, although proliferation rate cells was not affected. Furthermore,...
Abstract Heat shock can induce either cytoprotective mechanisms or cell death. We found that in certain human and mouse cells, including spermatocytes, activated heat factor 1 (HSF1) binds to sequences located the intron(s) of PMAIP1 (NOXA) gene upregulates its expression which induces apoptosis. Such a mode activation is not dependent on p53. Therefore, HSF1 only activate genes encoding proteins, prevents apoptosis, but it also positively regulate proapoptotic gene, facilitates This could...
Elevated temperatures induce activation of the heat shock transcription factor 1 (HSF1) which in somatic cells leads to proteins synthesis and cytoprotection. However, male germ (spermatocytes) caspase-3 dependent apoptosis is induced upon HSF1 spermatogenic are actively eliminated.To elucidate a mechanism such diverse activity we carried out genome-wide transcriptional analysis control heat-shocked cells, either spermatocytes or hepatocytes. Additionally, identify direct molecular targets...
Heat Shock Factor 1 (HSF1) is a key regulator of gene expression during acute environmental stress that enables the cell survival, which also involved in different cancer-related processes. A high level HSF1 estrogen receptor (ER)-positive breast cancer patients correlated with worse prognosis. Here we demonstrated 17β-estradiol (E2), as well xenoestrogen bisphenol and ERα agonist propyl pyrazole triol, led to phosphorylation on S326 positive but not ERα-negative mammary cells. Furthermore,...
The binding of capacitated spermatozoa to the egg's extracellular coat and induction acrosome reaction are necessary for successful fertilization in mammals. Biogenesis is complicated, not all proteins involved this process known. In study, we have cloned a novel mouse gene, Spaca7, that expressed exclusively testes. During postnatal development, transcripts gene could be detected at very low level 18-day-old testes higher 21-day-old later, which corresponds an expansion round spermatids....
SPEN (spen family transcription repressor) is a nucleic acid-binding protein putatively involved in repression of gene expression. We hypothesized that could be general downregulation the during heat shock response mouse spermatogenic cells through its interactions with chromatin. documented predominant nuclear localization spermatocytes and round spermatids, which was retained after shock. Moreover, excluded from highly condensed Chromatin immunoprecipitation experiments clearly indicated...
Spermatocytes are among the most heat-sensitive cells and exposure of testes to heat shock results in their Heat Shock Factor 1 (HSF1)-mediated apoptosis. Several lines evidence suggest that pleckstrin-homology-like domain family A, member (PHLDA1) plays a role promoting shock-induced cell death spermatogenic cells, yet its precise physiological is not well understood. Aiming elucidate hypothetical PHLDA1 HSF1-mediated apoptosis we characterized expression mouse during normal development...
Searchable abstracts of presentations at key conferences in endocrinology ISSN 1470-3947 (print) | 1479-6848 (online)