A5084587551- Cancer, Hypoxia, and Metabolism
- Immune cells in cancer
- Angiogenesis and VEGF in Cancer
- Mesenchymal stem cell research
- Immunotherapy and Immune Responses
- Cancer Research and Treatments
- RNA Interference and Gene Delivery
- Tissue Engineering and Regenerative Medicine
- Cancer Cells and Metastasis
- Cancer Immunotherapy and Biomarkers
- Diabetic Foot Ulcer Assessment and Management
- Integrated Water Resources Management
- interferon and immune responses
- Peptidase Inhibition and Analysis
- Cancer-related molecular mechanisms research
- Ubiquitin and proteasome pathways
- Heat shock proteins research
- Cell Adhesion Molecules Research
- Heme Oxygenase-1 and Carbon Monoxide
- CAR-T cell therapy research
- Geology and Environmental Impact Studies
- Transgenic Plants and Applications
- Protein Degradation and Inhibitors
- Electrospun Nanofibers in Biomedical Applications
- Ferroptosis and cancer prognosis
The Maria Sklodowska-Curie National Research Institute of Oncology
2015-2025
Medical University of Silesia
2024
Jagiellonian University
2021
Miejskie Przedsiebiorstwo Wodociągów i Kanalizacji (Poland)
2013-2015
AGH University of Krakow
2014
International Institute for Molecular Oncology
2013
University Medical Center Utrecht
2012
Translational Research in Oncology
2011
Tumor-associated macrophages (TAMs) play a significant role in at least two key processes underlying neoplastic progression: angiogenesis and immune surveillance. TAMs phenotypic changes important tumor vessel abnormalization/ normalization. M2-like stimulate immunosuppression formation of defective blood vessels leading to progression. In contrast M1-like trigger response normalize irregular vascular network which should sensitize cancer cells chemo- radiotherapy lead growth regression....
Adipose tissue-derived mesenchymal stromal cells (ASCs) have been shown to exhibit some promising properties of their use in regenerative medicine as advanced therapy medicinal products (ATMP). However, different sources origin, methods isolation, and expansion procedures cause the laboratory clinical results difficult compare. ASCs were isolated from lipoaspirates cultured three medium formulations: αMEM DMEM a basal supplemented with 10% human platelet lysate (hPL) 20% fetal bovine serum...
Abstract Immunotherapy is one of the most promising anti-cancer treatment. It involves activating host's own immune system to eliminate cancer cells. Activation cGAS-STING pathway therapeutic approach for immunotherapy. However, in human clinical trials, targeting results insufficient or unsustainable anti-tumor response. To enhance its effectiveness, combination with other therapies seems essential achieve synergistic systemic The aim this study was evaluate whether STING agonist-cGAMP...
Background/Objectives: The stimulator of interferon genes (STING) is currently accepted as a relevant target for anti-cancer therapies. Besides encouraging results showing STING agonist-induced tumor growth inhibition, in some types tumors the effect less prominent. We hypothesized that higher levels cancer cells and possibility its activation determine greater response. As local administration agonists induces systemic reaction, we emphasized importance host tumor-induced hematological...
A protocol for the ultrasound-assisted synthesis of new biologically active betulin–bisphosphonate conjugates in friendly reaction conditions was reported.
Sulfonylureas (SUs)-a class of drugs primarily used to treat type 2 diabetes-have recently attracted interest for their potential anticancer properties. While some studies have explored the chemical modification or design new SU derivatives, our work instead centers on biological evaluations all commercially available SUs in combination with doxorubicin (DOXO). These antidiabetic agents act by stimulating insulin secretion via KATP channel inhibition, and because channels share structural...
Vascular disrupting agents as DMXAA inhibit tumor growth only for a short period of time followed by rapid regrowth. Among others, hypoxia and presence transcription factor HIF-1α are responsible tumors The aim our study was to investigate the inhibition murine melanoma combining two agents: anti-vascular - inhibitor digoxin explaining mechanism action this combination. After treatment size reduced limited time. 7 days regrowth observed number vessels increased especially in tumor's...
Vasculature targeting agents have been tested as cancer therapeutics for the past few years. Such therapy could be accomplished using, example, bifunctional (two-domain) peptides. RGD-4C-GG-D(KLAKLAK)2, a peptide designed by Ellerby and coworkers (1999) (full sequence: ACDCRGDCFCGGKLAKLAKKLAKLAK), binds selectively to alphaVbeta3 integrin receptors expressed in tumor neovasculature and, after internalization, effectively induces apoptosis of endothelial cells. The aim this study was examine...
Heat shock transcription factor 1 (HSF1), the major regulator of stress response, is frequently activated in cancer and has an apparent role malignant transformation. Here we analyzed influence over-expression a constitutively active transcriptionally-competent HSF1 mutant form on phenotypes mouse human melanoma cells. We observed that expression supported anchorage-independent growth vitro, metastatic spread animal model vivo, although proliferation rate cells was not affected. Furthermore,...
Adipose-derived mesenchymal stromal cells (ADSCs) are multipotent cells. The secrete a number of cytokines and growth factors show immunoregulatory proangiogenic properties. Their properties may be used to repair damaged tissues. aim our work is explain the muscle damage mechanism with utilization human adipose-derived (hADSCs). For hADSCs isolation, we subcutaneous adipose tissue collected during surgery. murine hind limb ischemia was as model. unilateral femoral artery ligation performed...
Aims The aim of the present study was to isolate mesenchymal stromal cells (MSC) with CD105+CD34- phenotype from human hearts, and investigate their therapeutic potential in a mouse model hindlimb ischemia myocardial infarction (MI). aimed also feasibility xenogeneic MSCs implantation. Methods Results MSC isolated hearts were multipotent cells. Separation limited heterogeneity originally cell population. secreted number anti-inflammatory proangiogenic cytokines (mainly IL-6, IL-8, GRO)....
Abstract Due to immunosuppressive properties and confirmed tropism towards cancer cells mesenchymal stromal (MSC) have been used in many trials. In our study we these as carriers of IL-12 the treatment mice with primary metastatic B16-F10 melanomas. has anti-cancer activity, induces a strong immune response against acts an anti-angiogenic agent. A major limitation use therapy is its systemic toxicity. The aim work was develop system which cytokine may be administered intravenously without...
The study aimed to check the effectiveness of anticancer therapy combining a vascular-disruptive drug (combretastatin phosphate, CA4P) and liposomal formulation chemotherapeutic (doxorubicin). CA4P was synthesized in our laboratory according previously described procedure. antivascular long-circulating doxorubicin-loaded liposomes were used treat B16-F10 murine melanoma experimental tumors. Seventy-four hours after administration, decrease number tumor blood vessels apparent necrotic areas...
Introduction Targeting tumor vasculature is an efficient weapon to fight against cancer; however, activation of alternative pathways rebuild the disrupted leads rapid regrowth. Immunotherapy that exploits host immune cells elicit and sustain potent antitumor response has emerged as one most promising tools for cancer treatment, yet many treatments fail due developed resistance mechanisms. Therefore, our aim was examine whether combination immunotherapy anti-vascular treatment will succeed in...
D-K6L9 peptide is bound by phosphatidylserine and induces necrosis in cancer cells. In our therapeutic experience, this peptide, when administered directly into B16-F10 murine melanoma tumors, inhibited their growth. Cessation of therapy results, however, tumor relapse. We aimed at developing a combined involving additional factors that would yield complete elimination cells experimental animals. To purpose, we employed glycyrrhizin, an inhibitor HMGB1 protein, BP1 interleukin (IL)-12....
Desorption electrospray ionization may be used as a fast and convenient method for analysis identification of lipids in the cell culture. Oxidative stress, which usually involves changes lipids, was model pathology to show utility this methodology. This paper addresses surface preparation culture slides, induction oxidative monolayer well optimization analysis. Advantages drawbacks were also discussed.
According to literature data, self-renewing, multipotent, and clonogenic cardiac c-Kit(+) progenitor cells occur within human myocardium. The aim of this study was isolate characterize from explanted hearts. Experimental material obtained 19 adult 7 pediatric patients. Successful isolation culture achieved for 95 samples (84.1%) derived five different regions the heart: right left ventricles, atrium, intraventricular septum, apex. average percentage cells, as assessed by FACS, ranged between...