Individually personalized reconstructive microsurgery is more and universally recognized applied as a one-time, part of few, or even the only highly effective treatment patients with locally advanced midface cancer. Among increasing number publications focused on this field, most present different techniques used for single patient (case reports), fewer papers evaluate group including than 100 patients. A dataset 119 cancers in stage T3 T4 was to analyze whether there any correlation between...

Evaluate the possibility of performing a complex vascular allotransplant all neck organs including skin.There are 2 previous attempts described in literature, none them being that complex. The first one is nonfunctional due to chronic rejection, second viable yet considerably limited complexity (no parathyroids, no skin).The allotransplantation was performed simultaneously on adjacent operating rooms, using microsurgical techniques.The patient's voice, breathing through mouth, swallowing,...

Adipose-derived mesenchymal stromal cells (ADSCs) are multipotent cells. The secrete a number of cytokines and growth factors show immunoregulatory proangiogenic properties. Their properties may be used to repair damaged tissues. aim our work is explain the muscle damage mechanism with utilization human adipose-derived (hADSCs). For hADSCs isolation, we subcutaneous adipose tissue collected during surgery. murine hind limb ischemia was as model. unilateral femoral artery ligation performed...

Oral cavity cancer is a type of head and neck squamous cell carcinoma (HNSCC) contributes to significant morbidity mortality each year. An epigenetic pathway transcriptional inactivation for many genes has been described in various cancers, including HNSCC. For our study, we selected which silencing caused by hypermethylation can promote development. In 75 primary HNSCC tumours paired surgical margins, investigated the methylation status p16, APC, MGMT, TIMP3 CDH1 gene promoters...

This is a retrospective study describing multi-stage protocol for the management of severe mandibular hypoplasia in craniofacial microsomia (CFM) with accompanying obstructive sleep apnea (OSA). Patients require reconstruction functionality and esthetical features. In cohort, reconstructions based on free fibular flaps (FFF) may be most effective way. aged 4-17 years were treated FFF, which initially improved respiratory function assessed polysomnography (AHI). next stages treatment cases...

Oxidative stress is one of several factors which contribute to the development colorectal carcinogenesis. The aim study was an assessment activity antioxidant enzymes in tumour and corresponding normal distal mucosa a group patients with adenocarcinoma. Samples were obtained during resection cancer from 47 aged between 26 82 years. average distance 4.49 cm. Activities enzymes: superoxide dismutase (SOD), glutathione reductase (GR), peroxidase (GPx), S-transferase (GST), catalase (CAT)...

BACKGROUND:The human face is a one-of-a-kind structure with unique morphology, complexity, and function, in which different subunits are not even similar to other parts of the body. Therefore, extended complex deficits usually difficult reconstruct, autologous tissue restoration generally able give satisfactory aesthetic functional outcome. The main goal allotransplantation restore symmetry, contour, appearance as well function face, especially control orbicularis oculi oris muscle...

Extended postoperative mandibular reconstructions due to orofacial disease involving the temporomandibular joint (TMJ) in immature patients remain a challenge as result of ongoing growth, which is usually affected by and treatment. Current reconstructive techniques based fully on alloplastic total replacement fail meet anatomical functional requirements for masticatory system speech development. Fourteen children aged 12.6 ± 2.6 with tumors or congenital deformities affecting mandible TMJ...

Ameloblastoma (AM) is the most common, locally aggressive odontogenic tumor. It comprises about 1% of all head and neck neoplasms. occurs mainly in young adults their 3rd 4th decade life. localizes mandible 80% cases. According to 2017 WHO classification, 4 types ameloblastoma can be distinguished: (previously referred as solid/multicystic-SMA; "conventional type" AM), unicystic (AM-UA), extraosseous/peripheral (AM-PA), malignant/metastatic (AM-MA). Solid, multicystic common type....

Background: Pressure ulcers pose significant challenges in terms of treatment, often exhibiting a low success rate and propensity for recurrence. Children with neurological impairments such as myelomeningocele those spinal injuries are particularly vulnerable to developing pressure ulcers. Despite advancements, achieving successful reconstruction remains formidable task. Common sites prone ulcer formation include the sacral ischial regions, well areas over bony prominences. Additionally,...

Primary malignant bone tumors are most commonly associated with mutilating surgical procedures that can significantly disturb the motor development of a young patient and frequently affiliated major postoperative complications. Unfortunately, despite available autologous tissue donor sites, artificial materials still used for reconstruction post-resection defects. Reconstructive microsurgery is increasingly recognized as an effective method functional reconstruction, creating possibility...

This is a retrospective chart review of outcomes maxillofacial microvascular free flap reconstructions in pediatric and adolescent patients. A single head neck surgical team performed all the included 136 procedures. Demographic patient data were recorded. Type transfer each recipient site major complications analyzed. Relevant influencing factors, such as age, gender etiology defect determined using ANOVA test χ² independence. Results indicate 90% success rate. No significant relation was...

Abstract Background Adipose-derived stromal cells (ADSCs) demonstrate ability to promote tissue healing and down-regulate excessive inflammation. ADSCs have been used treat critical limb ischemia in preclinical clinical trials, but still, there is little known about their optimal delivery strategy. To date, no direct analysis of different methods has performed the hindlimb model. Therefore, this study we focused on therapeutic efficacy a murine model ischemia. Methods For hADSCs isolation,...

Background: Maxillofacial microvascular free-flap reconstructions are significant interventions in the management of congenital defects, traumatic injuries, malignancies, and iatrogenic complications pediatric young adult patients. Craniofacial disorders within this demographic can result profound functional, cosmetic, psychosocial impairments, highlighting critical need for thorough investigation into factors that may influence procedural success postoperative quality life. This...

The current study aimed to examine the association between head and neck squamous cell carcinoma (HNSCC) infection with different human papillomavirus virus (HPV) subtypes, including analysis of promoter methylation several genes (APC, CDKN2A, MGMT, CDH1 TIMP3) correlation their mRNA expression in tumours surgical margins.In 47 patients a primary tumour oral cavity, HPV detection identification 33 subtypes was performed after previous DNA isolation using GenoFlow Array Test Kit.Fifteen...

Oral squamous cell carcinoma (OSCC) is one of the most commonly detected neoplasms worldwide. Not all mechanisms associated with cycle disturbances are known in OSCC. Examples genes involved control CDKN2A, MDM2, E2F2 and LTF. The aim this study was to examine possible association between LTF mRNA expression influence on clinical variables. group consisted 88 Polish patients. gene levels were assessed by quantitative reverse transcription PCR. We found no statistically significant...

Neovascularization, the process of new blood vessels formation in response to hypoxia induced signals, is an essential step during wound healing or ischemia repair. It follows as a cascade consecutive events leading and their subsequent remodeling mature functional state, enabling tissue regeneration. Any disruption stages neovascularization can lead chronic wounds impairment In study we try explain biological basis accelerated ischemic after adipose tissue-derived stromal cells (ADSCs)...

Functional and esthetic final reconstruction of the cleft maxilla is still challenging. Current reconstructive augmentation techniques do not provide sufficient bone soft tissue support for predictable rehabilitation with dental implants due to presence maxillary critical size defects deficiency, scaring poor vascularity. In this article protocol use 3D virtual surgical planning microvascular transfers presented. Twenty-five patients (8 male/17 female) aged 14–41 years old cleft-associated...

Head and neck squamous cell carcinoma (HNSCC) is one of the leading cancers by incidence worldwide. The risk these strictly associated with alkylation factors present in tobacco smoke. crucial role preventing DNA played O6-methylguanine-DNA methyltransferase (MGMT). Dysfunction or lack MGMT an increased cancer. aim study was to assess influence polymorphisms: Rs12917 Rs11016879 on HNSCC course. consisted 42 patients 58 healthy individuals. Case samples were taken from resected tumour tissue....