A5079380067- Metabolomics and Mass Spectrometry Studies
- Cancer, Hypoxia, and Metabolism
- Head and Neck Cancer Studies
- Advanced MRI Techniques and Applications
- Cancer, Lipids, and Metabolism
- Diet and metabolism studies
- Diabetic Foot Ulcer Assessment and Management
- Mesenchymal stem cell research
- Reproductive System and Pregnancy
- Estrogen and related hormone effects
- Virus-based gene therapy research
- Amino Acid Enzymes and Metabolism
- Restraint-Related Deaths
- Polyamine Metabolism and Applications
- Neurogenesis and neuroplasticity mechanisms
- Nuclear Receptors and Signaling
- Analytical Chemistry and Chromatography
- Ginseng Biological Effects and Applications
- Tissue Engineering and Regenerative Medicine
- Advanced Radiotherapy Techniques
- Advanced NMR Techniques and Applications
- Oral health in cancer treatment
- Pharmacological Effects and Toxicity Studies
- Animal Virus Infections Studies
- Nerve injury and regeneration
The Maria Sklodowska-Curie National Research Institute of Oncology
2016-2024
Centrum Onkologii
2017
University of Liège
2016
Maj Institute of Pharmacology
2005
Polish Academy of Sciences
2005
Nutritional treatment in head and neck squamous cell carcinoma cancer (HNSCC) patients undergoing radio-/chemo-radiotherapy (RT/CHRT) is complex requires a multidisciplinary approach. In this study the real-time dynamic changes serum metabolome during RT/CHRT HNSCC were monitored using NMR-based metabolomics. The main goal was to find metabolic markers that could help prevent of acute radiation sequelae (ARS) escalation. 170 treated radically with RT/CHRT. Blood samples collected weekly,...
Adipose-derived mesenchymal stromal cells (ADSCs) are multipotent cells. The secrete a number of cytokines and growth factors show immunoregulatory proangiogenic properties. Their properties may be used to repair damaged tissues. aim our work is explain the muscle damage mechanism with utilization human adipose-derived (hADSCs). For hADSCs isolation, we subcutaneous adipose tissue collected during surgery. murine hind limb ischemia was as model. unilateral femoral artery ligation performed...
Abstract Epilepsy in children is the most frequent, heterogeneous and difficult to classify chronic neurologic condition with etiology found 35–40% of patients. Our aim detect metabolic differences between epileptic no neurological abnormalities order define background for therapy monitoring. The studied group included 28 epilepsy patients (median age 12 months) examined a diagnostic protocol including EEG, videoEEG, 24-hour-EEG, tests inborn errors metabolism, chromosomal analysis molecular...
The tissue metabolomic characteristics associated with endometrial cancer (EC) at different grades were studied using high resolution (400 MHz) magic angle spinning (HR-MAS) proton spectroscopy. metabolic profiles obtained from 64 patients (14 grade 1 (G1), 33 2 (G2) and 17 3 (G3) tumors) compared the profile acquired 10 benign disorders. OPLS-DA revealed increased valine, isoleucine, leucine, hypotaurine, serine, lysine, ethanolamine, choline decreased creatine, creatinine, glutathione,...
Abstract The purpose of this work was to investigate the distinct and common metabolic features malignant benign thyroid lesions in reference non-transformed tissue from contralateral gland (chronic thyroiditis colloid goiter). 1 H HR MAS NMR spectra 38 lesions, 32 112 samples non-tumoral (32 chronic 80 goiter) were subjected both multivariate univariate analysis. increased succinate, glutamine, glutathione, serine/cysteine, ascorbate, lactate, taurine, threonine, glycine,...
In the present study, we analyze nuclear magnetic resonance (NMR) blood serum metabolic profiles of 106 head and neck squamous cell carcinoma (HNSCC) patients during radio (RT) concurrent radio-chemotherapy (CHRT). Four different fractionation schemes were compared. The samples collected weekly, from day before treatment until last week CHRT/RT. NMR spectra acquired on A Bruker 400 MHz spectrometer at 310 K analyzed using multivariate methods. Seven metabolites found significantly to be...
Abstract Ionizing radiation may cause cardiotoxicity not only at high, but even low (considered as harmless) doses, yet the molecular mechanisms of heart’s response to doses are clear. In this work, we used high-resolution nuclear magnetic resonance (NMR) spectroscopy detect early and late effects on metabolism murine hearts. The hearts C57Bl/6NCrl female mice were irradiated in vivo with single 0.2 Gy or 2 using 6 MV photons, then tissues collected 48 h 20 weeks after exposure. most...
Treatment of head and neck squamous cell carcinoma (HNSCC) has a detrimental impact on patient quality life. The rate recognized distress/depression among HNSCC patients ranges from 9.8% to 83.8%, the estimated prevalence depression receiving radiotherapy is 63%. Shorter overall survival also occurs in preexisting or depressive conditions. present study analyzes nuclear magnetic resonance (NMR) blood serum metabolic profiles during radio-/chemoradiotherapy correlates detected alterations...
Abstract Background The aim of this prospective study is to identify the biomarkers associated with effects induction chemotherapy (iCHT) in terms favorable/weaker response treatment locally advanced head and neck squamous cells carcinomas (LA-HNSCC). Methods studied group consisted 53 LA-HNSCC patients treated iCHT. tolerance was measured by Common Terminology Criteria for Adverse Events (CTCAE). evaluated clinical, fiberoptic radiological examinations made before after iCHT (the TNM...
Breast tumors constitute the complex entities composed of cancer cells and stromal components. The compositional heterogeneity should be taken into account in bulk tissue metabolomics studies. aim this work was to find relation between histological content 1H HR-MAS (high-resolution magic angle spinning nuclear magnetic resonance) metabolic profiles samples excised from breast peritumoral areas 39 patients diagnosed with invasive carcinoma. total number histologically verified specimens 140....
Purpose The purpose of this work was to examine the suitability VIPAR nd polymer gel–9.4 T magnetic resonance microimaging system for high spatial resolution dose distribution measurements. Methods samples (3 cm in outside diameter and 12 height) were exposed ionizing radiation by using a linear accelerator (Varian TrueBeam, USA; 6 MV x‐ray beam). In calibration stage, nine gel dosimeter vials irradiated water phantom homogenously doses from 1.5 30 Gy order obtain R2‒dose relation....
The role of induction chemotherapy (iCHT) in locally advanced head and neck squamous cell carcinoma (LA-HNSCC) is still to be established due high toxicity variable response rates. aim this retrospective study use NMR-based serum metabolomics predict the rates iCHT from pretreatment samples. studied group consisted 46 LA-HNSCC patients treated with iCHT. treatment was evaluated by clinical, fiberoptic, radiological examinations made before after proton nuclear magnetic resonance (1H NMR)...
In this work, the HR MAS NMR (high-resolution magic-angle spinning nuclear magnetic resonance) spectroscopy technique was combined with standard histological examinations to investigate metabolic features of high-grade serous ovarian cancer (HGSOC) a special focus on relation between profile and cell fraction. The studied group consisted 44 patients HGSOC 18 benign tumors. Normal tissue also excised from 13 control patients. profiles 138 specimens were acquired Bruker Avance III 400 MHz...
Abstract Objectives Application of non‐invasive imaging methods plays an important role in the assessment cellular therapy effects peripheral artery disease. The purpose this work was to evaluate kinetics MRI‐derived parameters characterizing ischaemic hindlimb muscle after administration human mesenchymal stromal cells derived from adipose tissue (hADSC) mice. Materials and MRI experiments were performed on a 9.4T Bruker system. measurement protocol included transverse relaxation time...
_____________________________________________________________________________________________________furthermore prolonged the latency to epithelial ulceration and reduced ulcer duration.Proliferation measurements with BrdU did not show any substantial effects of DS.The adherens junction protein β-catenin significantly increase during irradiation, which occurred earlier additional DS treatment.The hypoxia markers HIF-1α GLUT-1 showed a progressive irradiaton alone, which, however, was also...
The present study compares two groups of locally advanced patients with head and neck squamous cell carcinoma (LA-HNSCC) undergoing concurrent chemoradiotherapy (cCHRT), specifically those for whom it is a first-line treatment who have previously received induction chemotherapy (iCHT). crucial question whether iCHT serious burden during subsequent LA-HNSCC how affects the tolerance to cCHRT. Of 107 patients, 54 cisplatin-based prior were clinically monitored at weekly intervals from day...