A5069899408- Metabolomics and Mass Spectrometry Studies
- Cancer, Lipids, and Metabolism
- Effects of Radiation Exposure
- Head and Neck Cancer Studies
- Advanced Proteomics Techniques and Applications
- Extracellular vesicles in disease
- Mitochondrial Function and Pathology
- Cancer, Hypoxia, and Metabolism
- Lung Cancer Diagnosis and Treatment
- Radiation Therapy and Dosimetry
- Thermal properties of materials
- Chemotherapy-induced cardiotoxicity and mitigation
- Radiomics and Machine Learning in Medical Imaging
- Diet and metabolism studies
- Cell death mechanisms and regulation
- Carcinogens and Genotoxicity Assessment
- Heat shock proteins research
- Mass Spectrometry Techniques and Applications
- Prostate Cancer Treatment and Research
- Lung Cancer Research Studies
- Inflammatory Biomarkers in Disease Prognosis
- Autophagy in Disease and Therapy
- Circular RNAs in diseases
- Immune Cell Function and Interaction
- Cancer, Stress, Anesthesia, and Immune Response
The Maria Sklodowska-Curie National Research Institute of Oncology
2014-2024
Heidelberger Institut für Radioonkologie
2015
Heidelberg University
2015
Polish-Japanese Academy of Information Technology
2013-2014
Exosomes are membrane vesicles of endocytic origin that participate in inter-cellular communication. Environmental and physiological conditions affect composition secreted exosomes, their abundance potential influence on recipient cells. Here, we analyzed protein component exosomes released vitro from cells exposed to ionizing radiation (2Gy dose) compared content with control not irradiated FaDu originating human squamous head neck cell carcinoma were using LC-MS/MS approach. We have found...
Formalin-fixed paraffin-embedded (FFPE) tissue specimens constitute a highly valuable source of clinical material for retrospective molecular studies. However, metabolomic assessment such archival remains still in its infancy. Hence, there is an urgent need efficient methods enabling extraction and profiling metabolites present FFPE specimens. Here we demonstrate the methodology isolation primary from tissues; either fresh-frozen, formalin-fixed or mouse kidney were analysed compared this...
Cancer radiotherapy (RT) induces response of the whole patient’s body that could be detected at blood level. We aimed to identify changes induced in serum lipidome during RT and characterize their association with doses volumes irradiated tissue. Sixty-six patients treated conformal because head neck cancer were enrolled study. Blood samples collected before, about one month after end RT. Lipid extracts analyzed using MALDI-oa-ToF mass spectrometry positive ionization mode. The major...
Background The serum metabolome is a potential source of molecular biomarkers associated with the risk breast cancer. Here we aimed to analyze metabolites present in pre-diagnostic samples collected from healthy women participating Norwegian Trøndelag Health Study (HUNT2 study) for whom long-term information about developing cancer was available. Methods Women HUNT2 study who developed within 15-year follow-up period (BC cases) and age-matched stayed cancer-free were selected ( n =453...
Introduction The progression of solid cancers is manifested at the systemic level as molecular changes in metabolome body fluids, an emerging source cancer biomarkers. Methods We analyzed quantitatively serum metabolite profile using high-resolution mass spectrometry. Metabolic profiles were compared between breast patients (n=112) and two groups healthy women (from Poland Norway; n=95 n=112, respectively) with similar age distributions. Results Despite differences both cohorts controls, a...
Exosomes that are released by T cells key messengers involved in immune regulation. However, the molecular profiling of these vesicles, which is necessary for understanding their functions, requires isolation from a very heterogeneous mixture extracellular vesicles present human plasma. It has been shown exosomes produced could be isolated plasma capture using antibodies target CD3 antigen, component TCR complex all lymphocytes. Here, we demonstrate CD3(+) can used high-throughput proteomics...
The lipid profiles of body fluids reflect the general condition whole organism and can indicate existence certain diseases such as cancer. Qualitative quantitative assessment lipids in blood other could reveal novel biomarkers for early detection prognosis cancer; addition, it may be useful monitoring efficacy toxicity anticancer treatment. Consequently, lipidomics is an emerging approach cancer research. Phospholipids make up important class, which particularly suitable analyses by mass...
Anticancer treatment induces systemic molecular changes that could be detected at the level of biofluids. Understanding how human metabolism is influenced by these treatments crucial to predict individual response and adjust personalized therapies. Here, we aimed compare profiles metabolites in serum head neck cancer patients treated with concurrent chemo-radiotherapy, radiotherapy alone, or induction chemotherapy. Serum samples were analyzed a targeted quantitative approach using combined...
Molecular components of exosomes and other classes small extracellular vesicles (sEV) present in human biofluids are potential biomarkers with possible applicability the early detection lung cancer. Here, we compared lipid profiles serum-derived sEV from three groups cancer screening participants: individuals without pulmonary alterations, benign nodules, patients screening-detected (81 each group). Extracellular particles were purified serum by size-exclusion chromatography, a fraction...
Cardiovascular disease is recognized as an important clinical problem in radiotherapy and radiation protection. However, only few radiobiological models relevant for assessment of cardiotoxic effects ionizing are available. Here we describe the isolation mouse primary cardiac endothelial cells, a possible target radiation. Cells isolated from hearts juvenile mice were cultured irradiated vitro. In addition, cells locally adult animals (up to 6 days after irradiation) tested. A dose-dependent...
Abstract Background In this exploratory study, the impact of local irradiation on systemic changes in stress and immune parameters was investigated eight patients treated with intensity-modulated radiation therapy (IMRT) or stereotactic ablative body radiotherapy (SABR) for prostate adenocarcinoma to gain deeper insights into how (RT) modulates system. Patients methods RT-qPCR, flow cytometry, metabolomics, antibody arrays were used monitor a panel stress- immune-related before RT, after...
Serum metabolome is a promising source of molecular biomarkers that could support early detection lung cancer in screening programs based on low-dose computed tomography. Several panels metabolites differentiate patients and healthy individuals were reported, yet none them validated the population at high-risk developing cancer. Here we analyzed serum profiles participants two studies: MOLTEST-BIS (Poland, n = 369) SMAC-1 (Italy, 93). Three groups included: patients, with benign pulmonary...
Radiotherapy causes molecular changes observed at the level of body fluids, which are potential biomarker candidates for assessment radiation exposure. Here we analyzed radiotherapy-induced in a profile small metabolites detected sera head and neck cancer patients using gas chromatography coupled with mass spectrometry approach. There were about 20 compounds, including carboxylic acids, sugars, amines amino whose levels significantly differed between pre-treatment post-treatment samples....
The study aimed to investigate late radiation-induced changes in the histology, ultrastructure, and activity of lysosomal enzymes mouse liver exposed ionizing radiation. experiment was conducted on C57BL/6J male mice whose distal part occasionally single doses radiation (6 MV photons) during targeted heart irradiation; estimated delivered analyzed tissue were 0.025 Gy, 0.25 1 2 Gy. Tissues collected 40 weeks after irradiation. We have observed that effects an adaptive nature their intensity...
Conformal intensity-modulated radiation therapy (IMRT) involves irradiation of large volume normal tissue with low and medium doses, biological relevance which is not clear yet. Serum proteome features were used here to study the dose-volume effects in patients irradiated IMRT due head neck cancer.Blood samples collected before during RT, also about one month year after end RT a group 72 who received definitive treatment. profiles analyzed using MALDI-ToF mass spectrometry 800-14,000 Da...
Biological aging is associated with various morphological and functional changes, yet the mechanisms of these phenomena remain unclear in many tissues organs. Hyperlipidemia among factors putatively involved liver heart. Here, we analyzed morphological, ultrastructural, biochemical features adult (7-month-old) elderly (17-month-old) mice, then compared age-related between wild type (C57Bl/6 strain) ApoE-deficient (transgenic ApoE-/-) animals. Increased numbers damaged mitochondria,...
Radiomics is an emerging approach to support the diagnosis of pulmonary nodules detected via low-dose computed tomography lung cancer screening. Serum metabolome a promising source auxiliary biomarkers that could help enhance precision in CT-based Thus, we aimed verify whether combination these two techniques, which provides local/morphological and systemic/molecular features disease at same time, increases performance classification models. The collected cohort consists 1086 patients with...
Improvement of radiotherapy techniques reduces the exposure normal tissues to ionizing radiation. However, risk radiation-related late effects remains elevated. In present study, we investigated long-term radiation on heart muscle morphology.We established a mouse model study microvascular density (MVD), deposition collagen fibers, and changes in accumulation heat shock 70 kDa protein 1 (HSPA1) irradiated tissue. Hearts C57BL/6 mice received single dose X‑ray range 0.2-16 Gy. Analyses were...