A5082972097- Metabolomics and Mass Spectrometry Studies
- Cancer, Lipids, and Metabolism
- Cancer, Hypoxia, and Metabolism
- Prostate Cancer Treatment and Research
- Advanced MRI Techniques and Applications
- Prostate Cancer Diagnosis and Treatment
- Radiomics and Machine Learning in Medical Imaging
- Bioinformatics and Genomic Networks
- Cancer survivorship and care
- Pregnancy and preeclampsia studies
- Diet and metabolism studies
- MRI in cancer diagnosis
- Mass Spectrometry Techniques and Applications
- Lipoproteins and Cardiovascular Health
- Cancer Genomics and Diagnostics
- Medical Imaging Techniques and Applications
- Spectroscopy and Chemometric Analyses
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Advanced Proteomics Techniques and Applications
- Nutrition, Genetics, and Disease
- Adipose Tissue and Metabolism
- Gene expression and cancer classification
- Cardiac Imaging and Diagnostics
- Breast Cancer Treatment Studies
- Reproductive System and Pregnancy
Norwegian University of Science and Technology
2016-2025
St Olav's University Hospital
2010-2024
Oslo University Hospital
2023
Levanger Hospital
2010
Molde Hospital
2009
Separating indolent from aggressive prostate cancer is an important clinical challenge for identifying patients eligible active surveillance, thereby reducing the risk of overtreatment. The purpose this study was to assess aggressiveness by metabolic profiling prostatectomy tissue and identify specific metabolites as biomarkers aggressiveness. Prostate samples (n = 158, 48 patients) with a high content (mean: 61.8%) were obtained using new harvesting method, profiles representing different...
The aims of this study were to characterize the metabolite profiles triple negative breast cancer (TNBC) and investigate associated with human epidermal growth factor receptor-2/neu (HER-2) overexpression using ex vivo high resolution magic angle spinning magnetic resonance spectroscopy (HR MAS MRS). Metabolic alterations caused by different estrogen receptor (ER), progesterone (PgR) HER-2 statuses also examined. To metabolic differences between two distinct groups, TNBC tumors compared...
Abstract Understanding how metabolite levels change over the 24 hour day is of crucial importance for clinical and epidemiological studies. Additionally, association between sleep deprivation metabolic disorders such as diabetes obesity requires investigation into links metabolism. Here, we characterise time-of-day variation effects on urinary profiles. Healthy male participants (n = 15) completed an in-laboratory study comprising one h sleep/wake cycle prior to continual wakefulness under...
Breast cancer is a heterogeneous disease at the clinical and molecular level. In this study we integrate classifications extracted from five different levels in order to identify integrated subtypes. Tumor tissue 425 patients with primary breast Oslo2 was cut blended, divided into fractions for DNA, RNA protein isolation metabolomics, allowing acquisition of representative comparable data. Patients were stratified groups based on their tumor characteristics levels, using various clustering...
An individualised risk-stratified screening for prostate cancer (PCa) would select the patients who will benefit from further investigations as well therapy. Current detection methods suffer low sensitivity and specificity, especially separating PCa benign prostatic conditions. We have investigated use of metabolomics analyses blood samples controls with hyperplasia (BPH).Blood plasma serum 29 patient 21 BPH were analysed by analysis using magnetic resonance spectroscopy, mass spectrometry...
Abstract Background Prostate cancer tissues are inherently heterogeneous, which presents a challenge for metabolic profiling using traditional bulk analysis methods that produce an averaged profile. The aim of this study was therefore to spatially detect metabolites and lipids on prostate tissue sections by mass spectrometry imaging (MSI), method facilitates molecular heterogeneous sections, can subsequently be related the histology same section. Methods Here, we simultaneously obtained...
Abstract Aims/hypothesis We determined whether the time of day exercise training (morning vs evening) would modulate effects consumption a high-fat diet (HFD) on glycaemic control, whole-body health markers and serum metabolomics. Methods In this three-armed parallel-group randomised trial undertaken at university in Melbourne, Australia, overweight/obese men consumed an HFD (65% energy from fat) for 11 consecutive days. Participants were recruited via social media community advertisements....
Pregnancy implies delicate immunological balance between two individuals, with constant changes and adaptions in response to maternal capacity fetal demands. We performed cytokine profiling of 1149 longitudinal serum samples from 707 pregnant women map first trimester term beyond. The levels 22 cytokines C-reactive protein (CRP) followed diverse but characteristic trajectories throughout pregnancy, consistent staged adaptions. Eotaxin showed a particularly robust decrease pregnancy. A strong...
Axillary lymph node status together with estrogen and progesterone receptor are important prognostic factors in breast cancer. In this study, the potential of using MR metabolomics for prediction these was evaluated. Biopsies from cancer patients (n = 160) were excised during surgery analyzed by high resolution magic angle spinning spectroscopy (HR MAS MRS). The spectral data preprocessed variable stability (VAST) scaled, training test sets generated Kennard-Stone SPXY sample selection...
Today's clinical diagnostic tools are insufficient for giving accurate prognosis to breast cancer patients. The aim of our study was examine the tumor metabolic changes in patients with locally advanced caused by neoadjuvant chemotherapy (NAC), relating these treatment response and long-term survival. Patients (n = 89) participating a randomized open-label multicenter were allocated receive either NAC as epirubicin or paclitaxel monotherapy. Biopsies excised pre- post-treatment, analyzed...
In this study, the feasibility of high resolution magic angle spinning (HR MAS) magnetic resonance spectroscopy (MRS) small tissue biopsies to distinguish between tumor and non-involved adjacent was investigated. With current methods, delineation borders during breast cancer surgery is a challenging task for surgeon, significant number re-surgeries occur. We analyzed 328 samples from 228 patients using HR MAS MRS. Partial least squares discriminant analysis (PLS-DA) applied discriminate...
The heterogeneous biology of breast cancer leads to high diversity in prognosis and response treatment, even for patients with similar clinical diagnosis, histology, stage disease. Identifying mechanisms contributing this heterogeneity may reveal new targets or clinically relevant subgroups treatment stratification. In study, we have merged metabolite, protein, gene expression data from examine the at a molecular level. study included primary tumor samples 228 non-treated patients....
Abstract Reactive stroma is a tissue feature commonly observed in the tumor microenvironment of prostate cancer and has previously been associated with more aggressive tumors. The aim this study was to detect differentially expressed genes metabolites according reactive content measured on exact same sample. evaluated using histopathology from 108 fresh frozen samples gathered 43 patients after prostatectomy (Biobank1). A subset analyzed both for metabolic (n = 85) transcriptomic alterations...
Abstract Background Colorectal cancer (CRC) is often diagnosed in advanced stages. Circulating tumour DNA (ctDNA) has been proposed as an early diagnostic biomarker. However, a screening tool, ctDNA mainly studied selected populations at the time of clinical diagnosis. The aim this study was to detect CRC by known markers up 2 years prior Methods In case–control study, methylated were detected plasma samples from 106 healthy controls and individuals with within 24 months following...
Breast cancer (BC) is a leading cause of cancer-related death worldwide. The diverse nature and heterogeneous biology BC pose challenges for survival prediction, as patients with similar diagnoses often respond differently to treatment. Clinically relevant intrinsic subtypes have been established through gene expression profiling are implemented in the clinic. While these show significant association clinical outcomes, their long-term prediction beyond 5 years deviates from expected...
Breast cancer is a heterogeneous disease with variable prognosis. Clinical factors provide some information about the prognosis of patients breast cancer; however, there need for additional to stratify improved and more individualized treatment. The aim this study was examine relationship between metabolite profiles tissue 5‐year survival. Biopsies from ( n = 98) were excised during surgery analyzed by high‐resolution magic angle spinning MRS. data multivariate principal component analysis...
Metabolic profiling of intact tumor tissue by high-resolution magic angle spinning (HR MAS) MR spectroscopy (MRS) provides important biological information possibly useful for clinical diagnosis and development novel treatment strategies. However, generation high-quality data requires that sample handling from surgical resection until analysis is performed using systematically validated procedures. In this study, we investigated the effect postsurgical freezing delay time on global metabolic...
Patients with locally advanced breast cancer have a worse prognosis compared to patients localized tumors and require neoadjuvant treatment before surgery. The aim of this study was characterize the systemic metabolic effect chemotherapy in large primary cancers relate these changes response long-term survival. This included 132 randomized receive or without addition antiangiogenic drug Bevacizumab. Tumor biopsies serum were collected during and, additionally 6 weeks after Samples analyzed...
MALDI MS imaging (MSI) is a powerful analytical tool for spatial peptide detection in heterogeneous tissues. Proper sample preparation crucial to achieve high quality, reproducible measurements. Here we developed an optimized protocol spatially resolved proteolytic with time-of-flight MSI of fresh frozen prostate tissue sections. The parameters tested included four different washes, methods protein denaturation, trypsin digestion (different densities, sprayers, and incubation times), five...
Robust biomarkers that identify prostate cancer patients with high risk of recurrence will improve personalised care. In this study, we investigated whether tissue metabolites detectable by high-resolution magic angle spinning magnetic resonance spectroscopy (HR-MAS MRS) were associated following radical prostatectomy. We performed a retrospective ex vivo study using HR-MAS MRS on samples from 110 prostatectomy specimens obtained three different Norwegian cohorts collected between 2002 and...
Acetaminophen (APAP) is one of the most commonly used analgesics worldwide, and overdoses are associated with lactic acidosis, hepatocyte toxicity, acute liver failure due to oxidative stress mitochondrial dysfunction. Hepatoma cell lines typically lack CYP450 activity generate reactive metabolite APAP observed in vivo, but still subject cytotoxicity. In this study, we employed metabolic profiling isotope labelling approaches investigate impact exposure cytotoxic doses on widely HepG2 model....