A5039384680- Effects of Radiation Exposure
- Aquaculture Nutrition and Growth
- Peroxisome Proliferator-Activated Receptors
- Cholesterol and Lipid Metabolism
- Aquaculture disease management and microbiota
- Carcinogens and Genotoxicity Assessment
- Acute Myeloid Leukemia Research
- Lipid metabolism and biosynthesis
- Reproductive biology and impacts on aquatic species
- DNA Repair Mechanisms
- Cancer, Stress, Anesthesia, and Immune Response
- Radiation Therapy and Dosimetry
- Hematopoietic Stem Cell Transplantation
- Diet, Metabolism, and Disease
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Adipose Tissue and Metabolism
- Cancer, Lipids, and Metabolism
- Biomarkers in Disease Mechanisms
- Radiation Dose and Imaging
- Molecular Biology Techniques and Applications
- Genomics and Phylogenetic Studies
- Acute Lymphoblastic Leukemia research
- Extracellular vesicles in disease
- Adipokines, Inflammation, and Metabolic Diseases
UK Health Security Agency
2021-2024
Public Health England
2018-2021
Devon History Society
2021
Universitat de Barcelona
2009-2014
University of Utah
2012-2014
To find new genes that influence liver lipid mass, we performed a genetic screen for zebrafish mutants with hepatic steatosis, pathological accumulation of fat. The red moon ( rmn ) mutant develops steatosis as maternally deposited yolk is depleted. Conversely, suppressed in by adequate nutrition. Adult show increased neutral lipids and induction biosynthetic when fasted. Positional cloning the locus reveals loss-of-function mutation slc16a6a solute carrier family 16a, member 6a ), gene...
Previous investigations in gene expression changes blood after radiation exposure have highlighted its potential to provide biomarkers of exposure. Here, FDXR transcriptional were investigated humans undergoing a range external procedures covering several orders magnitude (cardiac fluoroscopy, diagnostic computed tomography (CT)) and treatments (total body local radiotherapy). Moreover, method was developed assess the dose using physical parameters. significantly up-regulated 24 hr...
Here we present the presence of adiponectin and receptors [type 1 (adipoR1) type 2 (adipoR2)] in rainbow trout (Oncorhynchus mykiss) tissues cell cultures together with response to different scenarios. In fasting, expression was up-regulated adipose tissue, while its increased white red muscle. Insulin injection decreased adipoR1 muscles. We deduce that adipoRs muscle show opposite responses increasing insulin plasma levels, which may maintain sensitivity this tissue. Adiponectin inhibited...
Liver X receptors (Lxrs) are master regulators of cholesterol catabolism, driving the elimination from periphery to lumen intestine. Development pharmacological agents activate Lxrs has been hindered by synthetic Lxr agonists' induction hepatic lipogenesis and hypertriglyceridemia. Elucidating function in regulating enterocyte lipid handling might identify novel aspects metabolism that pharmacologically amenable. We took a genetic approach centered on single gene nr1h3 zebrafish study role...
In the event of a large-scale leading to acute ionizing radiation exposure, high-throughput methods would be required assess individual dose estimates for triage purposes. Blood-based gene expression is broad source biomarkers exposure which have great potential providing rapid large population. Time crucial component in radiological emergencies and shipment blood samples relevant laboratories presents concern. this study, we performed nanopore sequencing analysis determine if technology can...
The effects of a double replacement fish oil (FO) and meal (FM) by dietary vegetable ingredients in juvenile gilthead sea bream (Sparus aurata L. 1758) on some indices lipid metabolism plasma insulin levels were analysed. Four experimental diets with 75% FM plant proteins (PP) administered. Added was either FO (75PP/FO diet), or mix (VO), replacing 33%, 66% 100% (75PP/33VO, 75PP/66VO, 75PP/100VO diets). Another diet 50% substitution PP VO also tested (50PP/100VO diet). Final body weight...
Following cell stress such as ionising radiation (IR) exposure, multiple cellular pathways are activated. We recently demonstrated that ferredoxin reductase (FDXR) has a remarkable IR-induced transcriptional responsiveness in blood. Here, we provided first comprehensive FDXR variant profile following DNA damage. First, specific quantitative real-time polymerase chain reaction (qPCR) primers were designed to establish dose-responses for eight curated variants, all up-regulated after IR...
SUMMARY The present study aimed to analyze adiposity heterogeneity and the role of liver X receptor (LXRα) peroxisome proliferator-activated receptors(PPARs) as targets tumour necrosis factor-α (TNFα) in gilthead sea bream (Sparus aurata L.). screening 20 fish at beginning warm season identified two major groups with fat lean phenotypes. Fat showed increased mesenteric depots. This was concurrent adipose tissue enhanced expression lipoprotein lipase (LPL) whereas mRNA levels...
The effects of insulin and IGF-I on fatty acid (FA) glucose metabolism were examined using oleic or as tracers in differentiated rainbow trout (Oncorhynchus mykiss) myotubes. Insulin significantly reduced the production CO(2) from with respect to control values. also acid-soluble products (ASP) concentration FA medium, while cellular triacylglycerols (TAG) tended increase. Only produced a significant accumulation glycogen inside cells distribution experiments. Incubation catecholamines did...
External beam radiation therapy leads to cellular activation of the DNA damage response (DDR). double-strand breaks (DSBs) activate ATM/CHEK2/p53 pathway, inducing transcription stress genes. The dynamic nature this transcriptional has not been directly observed in vivo humans. In study we monitored messenger RNA transcript abundances nine damage-responsive genes (CDKN1A, GADD45, CCNG1, FDXR, DDB2, MDM2, PHPT1, SESN1, and PUMA), eight them regulated by p53 circulating blood leukocytes at...
For triage purposes following a nuclear accident, blood-based gene expression biomarkers can provide rapid dose estimates for large number of individuals. Ionizing-radiation-responsive genes are regulated through the DNA damage-response pathway, which includes activation multiple transcription factors. Modulators this pathway could potentially affect response these and consequently compromise accurate estimation calculations. In present study, four potential confounding factors were...
The ongoing SARS-CoV-2 pandemic has resulted in over 6.3 million deaths and 560 COVID-19 cases worldwide. Clinical management of hospitalized patients is complex due to the heterogeneous course COVID-19. Low-dose radiation therapy known dampen localized chronic inflammation been suggested be used reduce lung with However, it unknown whether alters response associated exposure related risk.We generated gene expression profiles from circulating leukocytes healthy donors.The p53 signaling...
Abstract Background In this exploratory study, the impact of local irradiation on systemic changes in stress and immune parameters was investigated eight patients treated with intensity-modulated radiation therapy (IMRT) or stereotactic ablative body radiotherapy (SABR) for prostate adenocarcinoma to gain deeper insights into how (RT) modulates system. Patients methods RT-qPCR, flow cytometry, metabolomics, antibody arrays were used monitor a panel stress- immune-related before RT, after...
Epidemiological studies have demonstrated an increased leukemia incidence following ionizing radiation exposure, but to date, the target cells and underlying mechanisms of leukemogenesis remain largely unidentified. We engineered a mouse model carrying different fluorescent marker on each chromosome 2, located inside minimum deleted region occurring after exposure recognized as first leukemogenic event. Using this tailored model, we report that more than half asymptomatic CBA Sfpi1 GFP/mCh...
Cancer and ionizing radiation exposure are associated with inflammation. To identify a set of radiation-specific signatures inflammation-associated genes in the blood partially exposed radiotherapy patients, differential expression 249 inflammatory was analyzed samples from cancer patients healthy individuals. The gene analysis on cohort 63 (endometrial, head neck, prostate cancer) before during (24 h, 48 ~1 week, ~4–8 weeks, 1 month after last fraction) identified 31 15 up- 16...
Abstract Therapy-related and more specifically radiotherapy-associated acute myeloid leukaemia (AML) is a well-recognized potential complication of cytotoxic therapy for the treatment primary cancer. The CBA mouse model used to study radiation leukaemogenesis mechanisms with Sfpi1/PU.1 deletion point mutation already identified as driving events during AML development. To identify new pathways, we analysed 123 radiation-induced (rAML) samples presence mutations previously in human found...
The RTGene study was focused on the development and validation of new transcriptional biomarkers for prediction individual radiotherapy patient responses to ionizing radiation. In parallel, purposes, this incorporated conventional radiation exposure, including dicentric assay. Peripheral blood samples were taken with ethical approval informed consent from a total 20 patients undergoing external beam breast, lung, gastrointestinal or genitourinary tumors. For assay, two each patient: prior...
Gene expression (GE) assays have shown great potential for rapid individual radiation dose exposure assessment. The aim of the present study was to optimise GE-based biological dosimetry protocols radiological emergencies. Experiments were carried out validate a newly developed protocol (P2) where several steps optimised and compare it with current validated in place our laboratory (P1). Several donor blood samples from exposed ex vivo following doses: 0, 0.5, 1, 2 Gy X-rays. Concomitant...