Dilated cardiomyopathy (DCMP) occupies a significant place among non-coronary heart diseases leading to chronic failure (CHF). Due the high mortality rate associated with DCMP, there is continuous search for alternative heart-preserving treatment methods as “bridges” transplantation. One of promising approaches stem cell therapy. Aim. To describe our own successful case intravenous transplantation autologous mesenchymal cells (MSCs) palliative CHF DCMP. Clinica case. A 48-year-old man DCMP...

External beam radiation therapy leads to cellular activation of the DNA damage response (DDR). double-strand breaks (DSBs) activate ATM/CHEK2/p53 pathway, inducing transcription stress genes. The dynamic nature this transcriptional has not been directly observed in vivo humans. In study we monitored messenger RNA transcript abundances nine damage-responsive genes (CDKN1A, GADD45, CCNG1, FDXR, DDB2, MDM2, PHPT1, SESN1, and PUMA), eight them regulated by p53 circulating blood leukocytes at...

Abstract Background Assessment of ‘physiological stress levels’ and ‘nutritional status’ before surgery is important for predicting complications indirect interventions on the pancreas. The aim this study was to determine neutrophil–lymphocyte ratio (NLR) nutritional risk index (NRI) indicators predict 90-day mortality in a cohort patients with complicated chronic pancreatitis cancer head Methods We evaluated preoperative levels NLR NRI among 225 subjects treated at different centres located...

This study aimed to investigate the effectiveness of combined negative pressure wound therapy (NPWT) and human amniotic membrane in patients with chronic wounds associated diabetes. A total five type 2 diabetes, including ischemic mixed forms diabetic foot syndrome, presenting lower extremities were included this study. Patients uncorrected limb ischemia excluded. The treatment protocol diabetes compensation (treatment fractional insulin therapy), anticoagulant, metabolic angiotropic...

<title>Abstract</title> This experimental study aimed to investigate the therapeutic potential of cellular cardiomyoplasty in context refractory angina using a rodent model. The comprised five groups animals subjected different interventions, including "empty" injections, autologous mesenchymal stem cell (MSC) injections via various routes, and untreated controls. Stress load modeling was employed assess hemodynamic response, mainly focusing on heart rate changes during isopropyl...

Aim. To experimentally study the effect of light different ranges on proliferation and migration mesenchymal stem cells human MSCs to select its optimal parameters for treatment. Materials methods. The experiment was conducted in vitro 260 cultures isolated from peripheral blood by magnetic separation. Cells were treated with pulsed LED light: 475 nm, 516 635 nm or left unstimulated. All devices had a peak radiation intensity 80 mW/cm2. average reached 40 Irradiation carried out at room...

Background. According to the Third International Consensus (Sepsis-3), sepsis is defined as a life-threatening organ dysfunction caused by an uncontrolled response of affected organism new infection, and septic shock sepsis-related condition characterized development circulatory failure accompanied arterial hypotension, with lactatemia requiring maintenance blood pressure vasopressors. Despite progress in diagnosing treating patients intra-abdominal infections, it continues be leading cause...

The purpose of this study was to investigate the possibility staged local treatment an infected mine-explosive wound using a gel containing Bacillus spp., cerium oxide nanoparticles, and cell therapy. Object Methods. article presents clinical case demonstrating effective 57-year-old patient with fragmentary fracture right femur large purulent thigh. underwent Ex-fix rod-type femur-tibia stabilization resection post-traumatic aneurysm superficial femoral artery. In first stage treatment, two...

Objective. To study the effectiveness of technology combined use negative pressure wound therapy and tissue in different phases process patients with diabetes mellitus. Materials methods. We analysed results treatment 18 type II mellitus grade IV Fontaine limb ischemia at Department Acute Vascular Diseases Zaitsev Institute General Emergency Surgery National Academy Medical Sciences Ukraine 2022–2024. Diagnostic angiography by Seldinger endovascular or hybrid revascularisation were...

Introduction. This study aims to investigate the quantitative criteria for damage blood vessels of skin and muscles in patients with ischemic form diabetic foot syndrome, goal determining degree microangiopathy assessing effect cellular revascularization on these indicators. Materials methods. For this research, paraffin block samples muscle tissue were obtained from 49 (research group) mixed forms accompanied by combined stenotic occlusive lesions arteries popliteal tibial segments. These...

Phosphorescence is considered one of the non-invasive glioblastoma testing methods based on studying molecular energy and metabolism L-tryptophan (Trp) through KP, which provides essential information regulating immunity neuronal function. This study aimed to conduct a feasibility using phosphorescence in clinical oncology as an early prognostic test detecting Glioblastoma. was conducted 1039 patients who were operated with follow-up between January 1, 2014, December 2022, retrospectively...

Breast cancers are very heterogeneous tissues constituted by epithelial cancer cells and an abnormal tumor microenvironment – cancer-associated fibroblasts (CAFs), activated adipocytes, mesenchymal stem (MSCs), others. The aim of the study is to their microenvironment, which behave like a complex metabolic ecosystem, where can reprogram metabolism as result interaction with components microenvironment. was based on (CSC) that were isolated from breast tumors magnetic separation (AutoMACS)....

Background. Diabetes mellitus affects almost all body systems, but the most dramatic complications occur in lower extremities. Diabetic foot syndrome takes leading position list of diabetes mellitus, to early disability and mortality.&#x0D; At present, a new technology - photodynamic therapy is being intensively developed worldwide. This technique can be used many different fields medicine, including purulent surgery.&#x0D; study aimed investigate possibility using 5-aminolevulinic acid as...

Background: Breast cancer (BC) is one of the most common malignancies and leading cause mortality in women Ukraine. The incidence BC conjunction with hepatitis C virus (HCV) increasing at a surprisingly rapid pace. aim current study was to compare mitochondrial complex between metastasis breast cancer patients virus. Materials Methods: 147 metastatic (MBC) we examined. Among them 102 were (MBC), 45 (MBCV). Results: total levels ATP ADP patient’s 1.25 ± 0.02 nmoles 2.27 0.03 cancer,...

Pediatric biobanks are an indispensable resource for the research needed to bring advances in personalized medicine into pediatric medical care. It is unclear how or when these care may reach children, but it unlikely that adults will be adequate. We conducted screening a hypothetic problem various European and American based on online surveys through e-mail distribution Biobank Economic Modeling Tool (BEMT) questionnaire model. Participants survey had work experience biobanking at least 3...

Premature ovarian insufficiency (POI) is condition of female hypogonadism. One the reasons for development POI anticancer chemotherapy. Currently, hormone replacement therapy considered most effective method treatment POI, which, however, had a number side effects, requires constant administration pharmaceuticals, did not restore fertility and correct pathological changes in target organs. Therefore, search alternative methods based on use stem cells promising. The aim work was to compare...

Étude expérimentale du rôle pathogénique de la NADPH-oxydase dans les tissus glande thyroïdeL'objectif l'étude était comparer le système NADPH / H2O2 et l'expression des enzymes, culture cellules thyroïde, en fonction sexe.Méthodes.La thyroïde a été prélevée chez donneuses adultes (n = 20) hommes 15).Les ont lavées deux fois avec HBSS centrifugées à 1000 xg pendant 5 minutes, puis cultivées 106 0,5 ml milieu par puits plaques 24 (Thermo Fisher Scientific, USA).Les niveaux déterminés manière...

Objective - to analyze the mechanisms of participation and relationship lipid andprotein metabolism in energy supply organism under physiological conditionsand pathology by examples consideration long adaptive reactions, especially inchronic stress. Relationships protein be considered from thepoint view functional-metabolic continuum.One functional blocks homeostasis is continuum (FMC),which provide body's functions (cells) with an adequate amount energy-plasticsubstrates. In implementation...

Objective. Elaboration of method for obtaining nonneuronal cultures astrocytes, derivatives from human mesenchymal stem cells various origin. Materials and methods. Mesenchymal were obtained peripheral umbilical blood in accordance to standard procedure, using the immune-magnet separation distributed into three groups, depending on primary material origin cellular differentiation technique applied: cells, which if our modified (CBMSC) used, used was (PBMSC-1) (PBMSC-2) protocols. As...

Objective. Determination of efficacy the cellular therapy modified method in chronic wounds on background diabetes mellitus, comparing with standard and new methods treatment experiment.&#x0D; Materials methods. Experimental investigations were conducted 30 adult male rats a Wistar line, which mellitus was experimentally simulated. Full-thickness square wound used as experimental pattern. In animals group A (control) processed 0.05% solution chlorhexidine bigluconate hydrohel bandages put...

Abstract Background Assessment of ‘physiological stress levels’ and ‘nutritional status’ before surgery is important for predicting complications indirect interventions on the pancreas. The aim this study was to determine neutrophil-lymphocyte ratio (NLR) nutritional risk index (NRI) indicators predict 90-day mortality in a cohort patients with complicated chronic pancreatitis cancer head Methods We evaluated preoperative levels NLR NRI among 225 subjects treated at different centres located...

Abstract Phosphorescence is considered one of the non-invasive glioblastoma testing methods based on studying molecular energy and metabolism L-tryptophan (Trp) through KP, which provides essential information regulating immunity neuronal function. This study aimed to conduct a feasibility using phosphorescence in clinical oncology as an early prognostic test detecting Glioblastoma. Materials Methods. was conducted 1039 patients who were operated with follow-up between January 1, 2014,...

Abstract In particular, as part of the extensive Stem Line project Mito-Cell-UAB073 «Stem cell lines - Quality control», we have taken a specific interest in new and complementary CQ approach to line stem intelligence Biobank. We combined computer vision image processing methods deep learning techniques create non-invasive Life Cell AI UAB model for robust prediction viability, using single static images obtained from standard optical light microscope systems. The showed sensitivity 82.1%...