A5063763655- Chronic Myeloid Leukemia Treatments
- Acute Lymphoblastic Leukemia research
- Chronic Lymphocytic Leukemia Research
- Acute Myeloid Leukemia Research
- Renal and related cancers
- Lymphoma Diagnosis and Treatment
- Antifungal resistance and susceptibility
- Neutropenia and Cancer Infections
- DNA Repair Mechanisms
- Eosinophilic Disorders and Syndromes
- Effects of Radiation Exposure
- Biomarkers in Disease Mechanisms
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Sepsis Diagnosis and Treatment
- Telomeres, Telomerase, and Senescence
- Cancer Risks and Factors
- Fungal Infections and Studies
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- Bacterial Identification and Susceptibility Testing
- Antibiotics Pharmacokinetics and Efficacy
- Vascular Tumors and Angiosarcomas
- Infectious Diseases and Mycology
Institute of Haematology and Blood Transfusion
2004-2025
Fakultní nemocnice Bulovka
2024
Charles University
2009-2020
Procalcitonin (PCT) and C-reactive protein (CRP) are established markers of infection in the general population. In contrast, several studies reported falsely increased PCT levels patients receiving T-cell antibodies. We evaluated validity these scheduled for hemopoietic stem cell transplantation anti-thymocyte globulin (ATG) during conditioning. also assessed renal liver functions their relationship to CRP changes. Twenty-six without clinical signs were prospectively studied. ATG was...
<title>Abstract</title> Adolescent and young adult (AYA) patients with chronic myeloid leukemia in phase (CML-CP) reportedly fare worse on tyrosine kinase inhibitor (TKIs) than adults. This real-life study compared mutation profiles outcomes 80 AYA, 97 adult, 16 pediatric CML-CP patients, alongside 81 <italic>BCR::ABL1</italic>-positive acute lymphoblastic (ALL) patients. Somatic mutations cancer-related genes (CRGs) were more frequent AYAs (25.0%) adults (19.6%) or children (12.5%). Ph+ ALL...
Following cell stress such as ionising radiation (IR) exposure, multiple cellular pathways are activated. We recently demonstrated that ferredoxin reductase (FDXR) has a remarkable IR-induced transcriptional responsiveness in blood. Here, we provided first comprehensive FDXR variant profile following DNA damage. First, specific quantitative real-time polymerase chain reaction (qPCR) primers were designed to establish dose-responses for eight curated variants, all up-regulated after IR...
Abstract Patients with lower-risk myelodysplastic syndromes (LR-MDS) have a generally favorable prognosis; however, small proportion of cases progress rapidly. This study aimed to define molecular biomarkers predictive LR-MDS progression and uncover cellular pathways contributing malignant transformation. The mutational landscape was analyzed in 214 patients, at least one mutation detected 137 patients (64%). Mutated RUNX1 identified as the main predictor rapid by statistics machine...
Abstract Several studies have reported that chronic myeloid leukaemia (CML) patients expressing e14a2 BCR::ABL1 a faster molecular response to therapy compared e13a2. To explore the reason for this difference we undertook detailed technical comparison of commonly used Europe Against Cancer (EAC) reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) assay in European Treatment and Outcome Study (EUTOS) reference laboratories ( n = 10). We found amplification ratio e13a2...
Overexpressed Wilms tumor gene 1 (WT1) has been found in a majority of patients with acute myeloid leukemia (AML).The aim this study was to confirm the applicability WT1 expression measurement as marker minimal residual disease (MRD).The measured by real-time polymerase chain reaction peripheral blood (PB) according European Leukemia Net (ELN) recommendations.The related reference Abelson (ABL) and results were calculated number copies 10 4 ABL gene.The upper normal limit set at 50...
Overall 42 patients (pts) transplanted in hematological CR1 were retrospectively analyzed. Median follow-up was 15 months (range 2-77). The expression of WT1 gene measured according to the European Leukaemia Net recommendations. At time allogeneic stem cell transplantation (allo-SCT) 29 pts WT1-negative and 13 WT1-positive. In univariate analysis, significantly better results observed group neg terms progression-free survival (in three yr 77% vs. 27%, p = 0.001). multivariate only...
Abstract Patients with myelodysplastic neoplasms (MDS) are classified according to the risk of acute myeloid leukemia transformation. Some lower‐risk MDS patients (LR‐MDS) progress rapidly despite expected good prognosis. Using diagnostic samples, we aimed uncover mechanisms this accelerated progression at transcriptome level. RNAseq was performed on CD34+ ribodepleted RNA samples from 53 LR‐MDS without (stMDS) and 8 who progressed within 20 months (prMDS); 845 genes were differentially...
Cancer and ionizing radiation exposure are associated with inflammation. To identify a set of radiation-specific signatures inflammation-associated genes in the blood partially exposed radiotherapy patients, differential expression 249 inflammatory was analyzed samples from cancer patients healthy individuals. The gene analysis on cohort 63 (endometrial, head neck, prostate cancer) before during (24 h, 48 ~1 week, ~4–8 weeks, 1 month after last fraction) identified 31 15 up- 16...
Invasive aspergillosis (IA) has been recognized as one of the leading infection-related cause death in hematologic patients (pts). Voriconazole approved for primary (first-line) therapy IA. However, outcomes remain poor, especially hematopoietic stem cell transplant (HSCT) pts. Existing limited data have documented improved survival rates with use combination therapy; succesfull application voriconazole and echinocandin also described. It seems to be rationale antifungal at beginning fungal...
In the last decade, geopolitical instability across globe has increased risk of a large-scale radiological event, when radiation biomarkers would be needed for an effective triage irradiated population. Ionizing elicits complex response in proteome, genome, and metabolome hence can leveraged as rapid sensitive indicators irradiation-induced damage. We analyzed plasma total-body (TBI) leukemia patients (n = 24) nonhuman primates (NHPs; n 10) before 24 h after irradiation, we performed global...
Background: Treatment free remission (TFR) or molecular relapse survival (MRFS) have become important therapeutic aims for patients with chronic myeloid leukemia (CML). This aim can be realised through achievement of a sustained deep response (DMR). Aims: In our previous work (Machova Polakova K, EHA 2018) we showed that BCR‐ABL1 DNA‐based approach residual disease monitoring may superior to mRNA analyses in significant number peripheral blood samples. this collaborative EUTOS study aimed...