A5077917038- Acute Myeloid Leukemia Research
- Drug Transport and Resistance Mechanisms
- Epigenetics and DNA Methylation
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Hemoglobinopathies and Related Disorders
- Cancer Genomics and Diagnostics
- Ovarian cancer diagnosis and treatment
- MicroRNA in disease regulation
- Renal and related cancers
- Acute Lymphoblastic Leukemia research
- Ethics and bioethics in healthcare
- RNA Interference and Gene Delivery
- Cancer, Hypoxia, and Metabolism
- Cholesterol and Lipid Metabolism
- Advanced biosensing and bioanalysis techniques
- Cardiovascular Syncope and Autonomic Disorders
- Metabolism and Genetic Disorders
- Cardiac Arrhythmias and Treatments
- HIV/AIDS drug development and treatment
- Molecular Biology Techniques and Applications
- Chronic Myeloid Leukemia Treatments
- RNA Research and Splicing
- Neuroendocrine Tumor Research Advances
- Multiple Myeloma Research and Treatments
- Neonatal Health and Biochemistry
Institute of Haematology and Blood Transfusion
2019-2024
University of New York in Prague
2022-2024
Charles University
2022-2024
National Institute of Public Health
2016-2023
To date, no chemoresistance predictors are included in acute myeloid leukaemia (AML) prognostic scoring systems to distinguish responding and refractory AML patients prior chemotherapy. ABC transporters have been described as altering chemosensitivity; however, a relevant study investigating their role at various molecular levels was lacking.
Abstract Background Multiple studies have reported the prognostic impact of DNA methylation changes in acute myeloid leukemia (AML). However, these epigenetic markers not been thoroughly validated and therefore are still considered clinical practice. Hence, we aimed to independently verify results selected describing relationship between specific genes their potential predicting overall survival (OS) event-free (EFS). Results Fourteen (published 2011–2019) comprising 27 were subjected...
Summary In this multi‐centre study, we analysed the prognostic impact of mutations in 19 genes associated with myeloid malignancies 258 newly diagnosed acute leukaemia patients (aged 19–70 years) undergoing intensive therapy. We identified five patient groups different risks and benefits from allogeneic hematopoietic stem cell transplantation (allo HSCT ) within intermediate cytogenetic risk group ( n = 184). The most adverse prognosis was observed DNMT 3A FLT 3 ‐ ITD co‐mutation, whose...
Aberrant epigenetic patterns are a hallmark of acute myeloid leukemia (AML). Mutations in profound regulators DNMT3A and IDH1/2 often occur concurrently AML.The aim was to analyze DNA methylation, hydroxymethylation mRNA expression profiles AML with mutations (individually combinations).Infinium MethylationEPIC BeadChip (Illumina) covering 850,000 CpGs utilized. The validation hydroxy-/methylation data done by pyrosequencing. HumanHT-12 v4 Expression used for examination.Hierarchical...
Abstract Background Changes in DNA methylation are common events the pathogenesis of acute myeloid leukemia (AML) and have been repeatedly reported as associated with prognosis. However, studies integrating these numerous potentially prognostically relevant changes lacking. Therefore, we aimed for an overall evaluation epigenetic aberrations to provide a comprehensive NGS-based approach assessment AML prognostication. Results We designed sequencing panel targeting 239 regions (approx. 573 kb...
Background: Approximately 40 % of acute myeloid leukemia (AML) patients does not achieve a complete hematologic remission (CR) after the first cycle 3 + 7 induction therapy and thus face an adverse prognosis. Mechanisms chemoresistance are still poorly understood, but drug export from cytosol plays crucial role. ABC transporters (ABCA2, ABCA5, ABCB1, ABCB6, ABCC1, ABCC3, ABCG2) were selected literature for expression, methylation, genotype efflux activity analysis. Aims: The aim this study...
<h3>Introduction/Background</h3> Membrane transporter proteins (for example ABC, SLC and ATPases) take part in oncogenesis the formation of chemoresistance, however interpretation their importance ovarian cancer prognosis is still limited. <h3>Methodology</h3> Gene expression profiling was performed on 39 ABC 12 transporters 3 ATPases epithelial (EOC) tissues results were assessed with respect to clinical presentation EOC patients. In a cohort primary (n = 57) control 14) we relative genes...