- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Genomics and Phylogenetic Studies
- Plant Disease Resistance and Genetics
- Microbial Community Ecology and Physiology
- Plant Pathogens and Fungal Diseases
- Plant-Microbe Interactions and Immunity
- Mycorrhizal Fungi and Plant Interactions
- Plant Pathogenic Bacteria Studies
- Identification and Quantification in Food
- Bacterial Identification and Susceptibility Testing
- Agronomic Practices and Intercropping Systems
- Wastewater Treatment and Nitrogen Removal
- Actinomycetales infections and treatment
- Microbial Natural Products and Biosynthesis
- Building materials and conservation
- Bacterial biofilms and quorum sensing
- Fungal Biology and Applications
- Salmonella and Campylobacter epidemiology
- Molecular Biology Techniques and Applications
- Antibiotic Resistance in Bacteria
- Nematode management and characterization studies
- Plant tissue culture and regeneration
- Biocrusts and Microbial Ecology
- Plant and Fungal Species Descriptions
National Institute of Applied Science and Technology
2016-2025
University of Carthage
2011-2025
Tunis University
2009-2021
Tunis El Manar University
2010-2021
Université Tunis Carthage
2012-2018
University of New Hampshire
2013-2015
Centre National des Sciences et Technologies Nucléaires
2015
Institut Pasteur de Tunis
2015
University of Jijel
2015
Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures
2015
Stones in arid environments are inhabited by actinobacteria of the family Geodermatophilaceae like genera Blastococcus and Modestobacter frequently isolated from altered calcarenites. Their habitat requires adaptation to light-induced other stresses that generate reactive oxygen species. Here, we show representative members species saxobsidens, Geodermatophilus obscurus, multiseptatus differentially adapted associated with environments. Whereas B. saxobsidens was found be sensitive gamma...
Abstract The repeated failures reported in cultivating some microbial lineages are a major challenge ecology and probably linked, the case of Frankia microsymbionts to atypical patterns auxotrophy. Comparative genomics so far uncultured cluster-2 Candidatus datiscae Dg1, with cultivated Frankiae has revealed genome reduction, but no obvious physiological impairments. A direct assay on nodule tissues from Coriaria myrtifolia infected closely-related strain permitted identification requirement...
Before the establishment of pure cultures, species Frankia alni, 'Frankia casuarinae' and elaeagni' were proposed to encompass all causal agents nitrogen-fixing root nodules dicotyledonous plants from genera Alnus, Casuarina or Elaeagnus. The sole with a validly published name, type F. was described by Woronin (1866) as present in alder. Until now no strain has been designated for even though absence seriously inhibited application modern taxonomic methods genus Frankia. Thus, we propose...
This study was designed to determine the plant growth promoting (PGP) potential of members genus Frankia. To this end, genomes 21 representative strains were examined for genes associated directly or indirectly with growth. All Frankia contained that encoded products biosynthesis auxins [indole-3-glycerol phosphate synthases, anthranilate phosphoribosyltransferases (trpD), and aminases (trpA B)], cytokinins (11 well-conserved within predicted biosynthetic gene cluster), siderophores,...
We report here the genome sequence of Frankia sp. strain CN3, which was isolated from Coriaria nepalensis. This is first fourth lineage Frankia, strains are unable to reinfect actinorhizal plants. At 10 Mb, it represents largest sequenced date.
In arid ecosystems environmental factors such as geoclimatic conditions and agricultural practices are of major importance in shaping the diversity functionality plant-associated bacterial communities. Assessing influence is a key to understand (i) driving forces determining shape root-associated communities (ii) plant growth promoting (PGP) services they provide. Desert oasis environment was chosen model ecosystem where agriculture possible by microclimate determined date palm cultivation....
Actinorhizal plants are able to establish a symbiotic relationship with Frankia bacteria leading the formation of root nodules. The interaction starts exchange signals in soil between plant and bacteria. This molecular dialogue involves signaling molecules that responsible for specific recognition host its endosymbiont. Here we studied two factors potentially involved casuarinae actinorhizal Casuarina glauca: (1) Root Hair Deforming Factor (CgRHDF) detected using test based on characteristic...
Summary Stone surfaces are extreme environments that support microbial life. This growth occurs despite unfavourable conditions associated with stone including limited sources of nutrients and water, high pH exposure to variations in temperature, humidity irradiation. These stone‐dwelling microbes often resistant desiccation, heavy metals, UV Gamma Here, we report on the effects climate geochemistry microbiomes Roman ruins North Africa. were dominated by Actinobacteria, Cyanobacteria...
The taxonomic position of strain BMG 8361
Phylogenetic analysis of the genus Aureimonas , utilizing both 16S rRNA gene sequences and comprehensive whole-genome data, revealed its polyphyletic nature, necessitating a revision to accommodate phylogenetically distinct species. Based on established threshold values for demarcation – specifically, similarity, Average Amino Acid Identity Percentage Conserved Proteins notably substantial divergence was observed within division into four genera is strongly supported. To address this, we...
Nutritional composition, antioxidant and antibacterial properties of Terfezia boudieri Chatin, a desert truffle largely distributed in Southern Tunisia were evaluated. Carbohydrates the most abundant macronutrients (62.03 g/100 g) followed by proteins (26.12 T. (dry mass). The ash content was 4.49 g dry mass potassium, phosphorus, iron, calcium found to be particularly boudieri. fat 8 mass, characterized its higher essential linoleic acid (n-6) (54.18%). Eight volatile compounds identified...
The Leishmania developmental life cycle within its sand fly vector occurs exclusively in the lumen of insect's digestive tract presence symbiotic bacteria. composition gut microbiota and factors that influence are currently poorly understood. A set factors, including host environment, may this composition. It has been demonstrated insect influences development several human pathogens, such as Plasmodium falciparum. For flies Leishmania, understanding interactions between parasite microbial...
ABSTRACT Frankia forms a nitrogen-fixing symbiosis with actinorhizal plants. We report draft genome sequence for sp. strain BCU110501, actinobacterium isolated from nodules of Discaria trinevis grown in the Patagonia region Argentina.
A novel Gram-reaction-positive, aerobic actinobacterium, tolerant to mitomycin C, heavy metals, metalloids, hydrogen peroxide, desiccation, and ionizing- UV-radiation, designated G18 T , was isolated from dolomitic marble collected outcrops in Samara (Namibia). The growth range 15–35°C, at pH 5.5–9.5 presence of 1% NaCl, forming greenish-black coloured colonies on GYM Streptomyces agar. Chemotaxonomic molecular characteristics the isolate matched those described for other representatives...