A5035432083- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Genomics and Phylogenetic Studies
- Plant Disease Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Microbial Community Ecology and Physiology
- Plant Pathogens and Fungal Diseases
- Actinomycetales infections and treatment
- Plant Pathogenic Bacteria Studies
- Bacterial Identification and Susceptibility Testing
- Identification and Quantification in Food
- Mycorrhizal Fungi and Plant Interactions
- Phytochemical and Pharmacological Studies
- Plant Pathogens and Resistance
- Plant Parasitism and Resistance
- Fungal Biology and Applications
- Building materials and conservation
- Microbial Natural Products and Biosynthesis
- Biocrusts and Microbial Ecology
- Wastewater Treatment and Nitrogen Removal
- Molecular Biology Techniques and Applications
- Protist diversity and phylogeny
- Agronomic Practices and Intercropping Systems
- Plant responses to water stress
- Medieval European Literature and History
Manouba University
2018-2025
National Institute of Applied Science and Technology
2016-2025
University of Carthage
2011-2025
Institut Supérieur de Biotechnologie de Sfax
2022
Tunis University
2011-2021
Tunis El Manar University
2012-2021
University of New Hampshire
2013-2015
Université Tunis Carthage
2013-2014
Abstract The repeated failures reported in cultivating some microbial lineages are a major challenge ecology and probably linked, the case of Frankia microsymbionts to atypical patterns auxotrophy. Comparative genomics so far uncultured cluster-2 Candidatus datiscae Dg1, with cultivated Frankiae has revealed genome reduction, but no obvious physiological impairments. A direct assay on nodule tissues from Coriaria myrtifolia infected closely-related strain permitted identification requirement...
Before the establishment of pure cultures, species Frankia alni, 'Frankia casuarinae' and elaeagni' were proposed to encompass all causal agents nitrogen-fixing root nodules dicotyledonous plants from genera Alnus, Casuarina or Elaeagnus. The sole with a validly published name, type F. was described by Woronin (1866) as present in alder. Until now no strain has been designated for even though absence seriously inhibited application modern taxonomic methods genus Frankia. Thus, we propose...
This study was designed to determine the plant growth promoting (PGP) potential of members genus Frankia. To this end, genomes 21 representative strains were examined for genes associated directly or indirectly with growth. All Frankia contained that encoded products biosynthesis auxins [indole-3-glycerol phosphate synthases, anthranilate phosphoribosyltransferases (trpD), and aminases (trpA B)], cytokinins (11 well-conserved within predicted biosynthetic gene cluster), siderophores,...
We report here the genome sequence of Frankia sp. strain CN3, which was isolated from Coriaria nepalensis. This is first fourth lineage Frankia, strains are unable to reinfect actinorhizal plants. At 10 Mb, it represents largest sequenced date.
Summary Stone surfaces are extreme environments that support microbial life. This growth occurs despite unfavourable conditions associated with stone including limited sources of nutrients and water, high pH exposure to variations in temperature, humidity irradiation. These stone‐dwelling microbes often resistant desiccation, heavy metals, UV Gamma Here, we report on the effects climate geochemistry microbiomes Roman ruins North Africa. were dominated by Actinobacteria, Cyanobacteria...
The taxonomic position of strain BMG 8361
ABSTRACT Frankia forms a nitrogen-fixing symbiosis with actinorhizal plants. We report draft genome sequence for sp. strain BCU110501, actinobacterium isolated from nodules of Discaria trinevis grown in the Patagonia region Argentina.
A novel Gram-reaction-positive actinobacterium, designated BMG 804T, was isolated from an archaeological Roman pool located in Gafsa, Tunisia. The strain grew as dry bright orange colonies at 30 °C and pH 6.0-8.0. It contained meso-diaminopimelic acid the cell wall. whole-cell sugars consisted of glucose, rhamnose ribose. Polar lipids present were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, unidentified glycolipid two phospholipids. MK-9(H4)...
The taxonomic description of a nitrogen-fixing actinobacterium, strain BMG5.1T, as novel species within the genus Frankia was based on polyphasic approach. isolated from root nodules Coriaria japonica, and it fulfilled Koch's postulates by inducing effective spp. Datisca Based phenotypic chemotaxonomic features, BMG5.1T is distinguishable all other . It characterized presence phosphatidylinositol, diphosphatidylglycerol, phosphatidylglycerol glycophospholipids in its polar lipids; galactose,...
The African BioGenome Project (AfricaBP) is a Pan-African initiative aimed at improving food systems and biodiversity conservation through genomics while ensuring equitable data sharing benefits. Open Institute the knowledge exchange platform of AfricaBP which aims to bridge local gaps in bioinformatics enable infrastructural developments. In 2024, advanced this mission by organising 31 workshops that attracted more than 3500 registered attendees trained 380 researchers genomics,...
Phylogenetic analysis of the genus Aureimonas , utilizing both 16S rRNA gene sequences and comprehensive whole-genome data, revealed its polyphyletic nature, necessitating a revision to accommodate phylogenetically distinct species. Based on established threshold values for demarcation – specifically, similarity, Average Amino Acid Identity Percentage Conserved Proteins notably substantial divergence was observed within division into four genera is strongly supported. To address this, we...
ABSTRACT Members of the actinomycete genus Frankia form a nitrogen-fixing symbiosis with 8 different families actinorhizal plants. We report draft genome sequence for sp. strain BMG5.12, actinobacterium isolated from Tunisian soils ability to infect Elaeagnus angustifolia and Myrica gale .
A black-pigmented, aerobic actinobacterium, tolerant to ionizing radiation, designated BMG 825T, was isolated from desert limestone dust in Tunisia. The strain grew within the temperature range 10-40 °C, at pH 5.5-11.0 and presence of 2 % NaCl. DNA G+C content 75.7 mol%, its cell-wall peptidoglycan contained meso-diaminopimelic acid. Sugars whole-cell hydrolysates were galactose, glucose, trace amounts ribose mannose. predominant menaquinone MK-9(H4), major fatty acids iso-C16 : 0 C16 1ω7c....
Nitrogen-fixing actinobacteria of the genus Frankia are symbionts woody dicotyledonous plants termed actinorhizal plants. We report here a 5.27-Mbp draft genome sequence for sp. strain BMG5.23, salt-tolerant nitrogen-fixing actinobacterium isolated from root nodules Casuarina glauca collected in Tunisia.
The taxonomic status of strain M16386T, a nitrogen-fixing but non-nodulating isolate from Morella californica, was established on the basis polyphasic approach. grows as branched hyphae, with vesicles and non-motile productive multilocular sporangia. It metabolizes short fatty acids, TCA cycle intermediates carbohydrates carbon sources, fixes nitrogen in absence combined source growth media. Chemotaxonomic traits M16386T are consistent its affiliation to genus Frankia. characteristic diamino...