This study investigated the effects of dietary sodium butyrate (SB) supplementation, provided as a specially coated product, on growth performance, intestinal development, morphological structure and function in broilers. In total, 720 one-day-old Arbor Acres male broilers were randomly allocated into six treatment groups with replicates each then fed basal diets (control) supplemented 0, 200, 400, 800 or 1000 mg/kg SB antibiotics (100 aureomycin 20 colistin sulfate). The trial lasted for 42...

Inflammatory bowel disease (IBD) is a chronic inflammatory of intestinal mucosa and submucosa, characterized by the disruption epithelial barrier, increased production mediators, excessive tissue injury. Intestinal cells, as well microvascular endothelial play important roles in IBD. To study potential effects kaempferol IBD progress, we established novel epithelial-endothelial cells coculture model to investigate inflammation barrier function. Data demonstrated an obvious transepithelial...

The gastrointestinal tract is exposed to pro-oxidants from food, host immune factors, and microbial pathogens, which may induce oxidative damage. Oxidative stress has been shown play an important role in the onset of inflammatory bowel disease. This study aimed use a novel model evaluate effects screened natural component explore its possible mechanism. An vitro Caco2 cell induced by H2O2 was established using real-time cellular analysis system verified addition glutathione (GSH). A variety...

Growing evidence of intestinal microbiota-muscle axis provides a possibility to improve meat quality broilers through regulating microbiota. Water-holding capacity is crucial factor evaluate the quality. High water-holding usually described as low drip-losing rate. This study aimed explore relationship between microbiota and muscle in broilers. According our results, two native breeds (the Arbor Acres Beijing-You broilers) exhibited remarkable differences composition. However, regular gut...

This study was conducted to evaluate the effects of quercetin on antioxidant ability, intestinal barrier functions, and cecal microbiota in broiler chickens fed with oxidized soya oil. Four hundred eighty male Arbor Acres broilers were randomly assigned 5 treatments, each involving 8 cages (12 birds per cage). The treatment groups as follows: control group, basal diets containing oil, oil supplemented 200 ppm quercetin, 400 800 quercetin. results showed that dietary supplementation at a dose...

We aimed to investigate whether dietary supplementation of methionine could mitigate intestinal oxidative injury in broilers under high stocking density (HSD). In the grower phase (d 22–42), 576 with similar body weight were randomly chosen and divided into 8 groups a 2 × 4 factorial experiment. Two different densities (14 20 per m2) tested levels: 0.35%, 0.4%, 0.45%, or 0.5%. Intestinal morphological stress markers assessed at end test period. The results showed that mortality was...

The development and utilization of probiotics had many environmental benefits for replacing antibiotics in animal production. Bacteria the intestinal mucosa have better adhesion to host epithelial cells compared bacteria contents. In this study, lactic acid were isolated from broiler chickens investigated as substitution antibiotic production.In addition resistance, high temperature antimicrobial sensitivity tests, cell adhesion, Enterococcus faecium PNC01 (E. PNC01) was showed be...

The intestinal dysfunction plays an important role in the decreased growth performance of broiler chickens under high stocking density. Gut microbiota maintaining health. However, modulation pathway gut by regulating barrier and histomorphology remains unknown. One hundred forty-four male Arbor Acres broilers (22-d-old) with similar weight were randomly assigned to two treatments: a (HSD, 20 broilers/m2) or low density treatment (LSD, 14 broilers/m2), six replicates per treatment....

Although previous studies show that exogenous nutrients regulate the stem cell function, little is known about effects of L-arginine on intestinal cells (ISCs). In this study, we utilize mice, small (SI) organoids, and ISC-Paneth co-cultured models to clarify role in ISC function. We find essential for ISCs proliferation epithelial renewal. Our data Paneth cells, a critical component niche, augment function response L-arginine. Moreover, enhanced expression Wnt3a which ligand Wnt/β-catenin...

Background: The prevalence of inflammatory bowel disease is rapidly increasing around the world. Quercetin a flavonoid commonly found in vegetables and fruits has been reported to exert numerous pharmacological activities such as enhancing antioxidant capacity or suppressing inflammation. Objective: We aimed explore whether quercetin was effective for IBD underlying mechanism ameliorative effects on DSS-induced colitis mice. Methods: Thirty-six mice were randomly assigned three treatments,...

In the egg production industry, trace elements are required as additional dietary supplements to play vital roles in performance and quality. Compared inorganic microelements (ITs), appropriate dose of organic (OTs) environmentally friendly sufficient satisfy needs hens. order evaluate extent which low-dose OTs replace whole ITs, effects copper, zinc, manganese, iron compound on performance, eggshell quality, antioxidant capacity, immune function, mineral deposition old laying hens were...

This study aimed to evaluate the efficacy of organic acids (OAs) in starter broilers and investigate whether supplemental OAs could alleviate high stocking density (HSD) stress condition grower broilers. A total 408 1-day-old Arbor Acres were assigned into two groups without or with liquid phase. In phase, each group phase was divided a normal an HSD. The OA dose 0.16% at phases. results showed that decreased chyme pH gizzard duodenum increased activities chymotrypsin α-amylase duodenal (p <...

Salmonella Typhimurium (S. Typhimurium) infection in broiler chickens threatens public health and livestock production. In this study, we explored the effects of highly nutritious (crude protein 21.8%, metabolizable energy 3.16 Mcal/kg) lowly 18.1%, 2.98 diets on S. by altering intestinal morphology environment chickens. The diet significantly increased body weight gain reduced feed conversion ratio day 1 to 21 (P < 0.01). promoted villus height, crypt depth, their improve epithelial...

Abstract BACKGROUND Trivalent chromium (Cr) is involved in carbohydrate, lipid, protein and nucleic acid metabolism animals. This study evaluated the effects of different organic Cr forms with methionine (CrMet), picolinate (CrPic), nicotinate (CrNic), yeast (Cr‐yeast) at level 400 μg kg −1 Cr, on growth performance, lipid metabolism, antioxidant status, breast amino fatty profiles broilers. In total, 540 one‐day‐old Arbor Acres male broilers were randomly assigned to five treatments six...

Although Iron (Fe) is an essential nutrient that plays a vital role in respiratory processes, excessive Fe the diet can affect health of broilers. We investigated effects supplemented with high levels iron chelates lysine and glutamic acid (Fe−LG) on growth performance, serum biochemical parameters, antioxidant status, duodenal mRNA expression transporters A total 800 1-day-old male Arbor Acres broilers were assigned to 5 groups, 8 replicates each. Broilers fed corn−soybean meal basal or...

In this study, we investigated the effect of dietary methionine restriction (MR) on antioxidant function and inflammatory responses in lipopolysaccharide (LPS)-challenged broilers reared at high stocking density. A total 504 one-day-old male Arbor Acre broiler chickens were randomly divided into four treatments: 1) CON group, fed a basal diet; 2) LPS LPS-challenged 3) MR1 methionine-restricted diet (0.3% methionine); 4) MR2 (0.4% methionine). intraperitoneally injected with 1 mg/kg body...

Abstract The renewal and repair of intestinal epithelium depend on the self-renewal stem cells (ISCs) under physiological pathological conditions. Although previous work has established that exogenous nutrients regulate adult cell activity, little is known about regulatory effect L-arginine ISCs. In this study we utilize mice small (SI) organoid models to clarify role epithelial differentiation We show increases expansion ISCs in mice. Furthermore, CD90 + stromal augment stem-cell function...

This study aimed to investigate the effects of dietary organic trace minerals on egg quality and intestinal microflora laying hens during late production stage. In total, 1,080 Jinghong-1 aged 57 weeks were randomly assigned five treatment groups: CON, basal diet containing about 6, 29, 49, 308 mg·kg-1 Cu, Mn, Zn, Fe; IT100, supplemented with 10, 80, 60 Fe (each as inorganic sulfates), respectively; OT20, 2, 16, 12 chelated lysine methionine in ratio 2:1 amino acid: minerals), OT30, 3, 24,...

In order to evaluate the effects of acidifiers and essential oils in drinking water on growth, intestinal digestive capacity, immune status broilers, a total 480, 1-day-old Arbore Acres broilers were randomly assigned four treatments including normal tap (Ctr) supplemented with acidifier I (ACI), (ACI+EO), II (ACII). Both ACI+EO ACII increased final body weight. The pH value crop gizzards was reduced by ACI+EO, decreased values proventriculus (

The composition of the bacterial community affects intestinal health and growth performance broiler chickens. main purpose this study was to explore effects flavomycin colistin sulfate on resistance Salmonella typhimurium infection, ileal bacteria health. In total, 396 1-day-old chickens were randomly divided into six groups. Two groups fed each one diets—the control diet (CON), at 10 mg/kg (AntiG+), 40 (AntiG−), for 5 days. Then, two challenged with S. 8th day; these named CONS, AntiG+S...

Plantago asiatica L. is widely distributed in Eastern Asia and a commonly used drug China, Korea, Japan for diuretic antiphlogistic purposes. In this experiment, the present study was performed to isolate antioxidant molecules based on DPPH scavenging activity assay discover bioactive compounds which contributed performing function of Each faction chosen further isolation guided by assay. Afterwards, two potential molecules, aesculetin apigenin, were isolated vitro cells....

This study was conducted to investigate the effects of different levels yeast chromium on growth performance, organ index, antioxidant capacity, immune performance and liver health broilers under high stocking density. A total 684 1-day-old Arbor Acres were selected fed a common diet from 1 22 days age. At end days, with similar weight randomly divided into six treatments, replications in each treatment. The control groups raised at low density (14 broilers/m2, LSD) (20 HSD). treatment diets...

Abstract Background Eggshell plays an important role in protecting against physical damage and microorganic invasion. It is subject to quality loss with increasing hen age, fragile eggshells result huge economic losses the poultry industry. Therefore, improving eggshell particularly important. However, little known about potential molecular mechanisms regulating chickens. Methods In this study, we aimed compare differential expression of long non-coding RNAs (lncRNAs) mRNAs between old young...

This study investigated whether variations in growth response to low nutrient density across breeds are linked microbiota regulation. Arbor Acres (