A5073611267- Enzyme Structure and Function
- Mechanical Circulatory Support Devices
- RNA Research and Splicing
- Health Systems, Economic Evaluations, Quality of Life
- Nuclear Structure and Function
- Fuel Cells and Related Materials
- Cardiac Structural Anomalies and Repair
- Pharmaceutical Economics and Policy
- Protein Structure and Dynamics
- ATP Synthase and ATPases Research
- Advanced Database Systems and Queries
- Glycosylation and Glycoproteins Research
- Pluripotent Stem Cells Research
- Cardiac Fibrosis and Remodeling
- Transplantation: Methods and Outcomes
- Digestive system and related health
- Congenital heart defects research
- CRISPR and Genetic Engineering
- HER2/EGFR in Cancer Research
- Medical Imaging Techniques and Applications
- Endoplasmic Reticulum Stress and Disease
- Biomedical Ethics and Regulation
- NMR spectroscopy and applications
- Growth Hormone and Insulin-like Growth Factors
- Law and Political Science
AstraZeneca (Germany)
2023
AstraZeneca (United Kingdom)
2021
Novartis (Switzerland)
2020
Boehringer Ingelheim (Austria)
2005-2011
Bayer (Germany)
2007-2010
Target (United States)
2010
Target (Germany)
2007-2009
Städtische Kliniken Mönchengladbach
2008
Philadelphia University
2008
Max Planck Society
2002-2003
Accurate testing of HER2 is centrally important for breast cancer therapy and prognosis. Immunohistochemistry (IHC) fluorescence in situ hybridization (FISH) are current standard methods. As a potential alternative assessment HER2, we explored quantitative real-time reverse transcription-PCR (RT-PCR), fast inexpensive method yielding results insensitive to interobserver variability amenable standardized scoring.We assessed status at the DNA, mRNA, protein levels with FISH, RT-PCR, IHC 136...
Abstract The contributions of backbone NH group dynamics to the conformational heat capacity B1 domain Streptococcal protein G have been estimated from temperature dependence 15 N NMR‐derived order parameters. Longitudinal ( R 1 ) and transverse 2 relaxation rates, cross‐relaxation rates (η xy ), steady state { H}‐ nuclear Overhauser effects were measured at temperatures 0, 10, 20, 30, 40, 50°C for 89–100% secondary amide nitrogen nuclei in domain. ratio /η was used identify which exchange...
Insulin-like growth factor (IGF) signaling is a key regulator of breast development and cancer. We have analyzed the expression IGF cascade in 17 human cancer 4 mammary epithelial cell lines. Five lines expressed high levels IGF1 receptor, insulin (INS)/IGF receptor substrate 1, IGF-binding proteins 2 4, as well estrogen (ESR), indicating co-activation ESR signaling. Next, we stably overexpressed IGF2 MCF7 cells, which did not affect their characteristics localization marker genes E-cadherin...
RanBP type proteins have been reported to increase the catalytic efficiency of RanGAP-mediated GTPase reaction on Ran. Since structure Ran-RanBP1-RanGAP complex showed RanBP1 be located away from active site, we reinvestigated using fluorescence spectroscopy under pre-steady-state conditions. We can show that indeed does not influence rate-limiting step reaction, which is cleavage GTP and/or release product Pi. It does, however, dynamics Ran-RanGAP interaction, its most dramatic effect being...
Mechanical unloading by ventricular assist devices (VAD) leads to significant gene expression changes often summarized as reverse remodeling. However, little is known on individual transcriptome during VAD support and its relationship nonfailing hearts (NF). In addition no data are available for the regulation nonpulsatile support. Therefore we analyzed patterns of 30 paired samples from VAD-supported (including 8 VADs) control (NF) using first total human genome array available. Transmural...
Abstract In the new era of healthcare digitalization, there is a golden opportunity in overlap between digital health and Real-World Evidence (RWE). this commentary, we define RWE investigate their intersection. We describe stages value chain critical to evidence generation process, how these change with technologies opportunities challenges that arise from evolve—including application for stakeholders such as patients, physicians regulators. also discuss current published guidelines...
Abstract This commentary is authored by several industry real-world evidence (RWE) experts, with support from IQVIA, as part of the 'RWE Leadership Forum': a group Industry Leaders who have come together non-competitive partners to understand and respond RWD/E challenges opportunities single expert voice. Here, forum discusses value in bridging disconnect between RTCs RWE, view promoting use RWE RCT environment. endpoints are explored along axes including their clinical relevance measure...
Cardiomyocytes derived from pluripotent embryonic stem cells (ESC) have the advantage of providing a source for standardized cell cultures. However, little is known on regulation genome during differentiation ESC to cardiomyocytes. Here, we characterize transcriptome mouse line CM7/1 into beating cardiomyocytes and compare gene expression profiles with those primary adult murine left ventricular myocardium. We observe that cardiac pattern fully differentiated CM7/1-ESC highly similar...
Abstract The trans-activator protein (Tat) of human immunodeficiency virus type 1 (HIV-1>) binds to an uridine-rich bulge RNA target (TAR; trans-activation responsive element) predominantly via its basic sequence domain. structure the Tat(46–58)-TAR complex has been determined by a novel modeling approach relying on structural information about one crucial arginine residue and crosslink data. strategy described here solely uses this experimental data without additional “modeling” assumptions...
The objective of this study was to gain insight into the biological basis colon cancer progression by characterizing gene expression differences between normal epithelium, corresponding colorectal primary tumors and metastases. We found a close similarity in patterns metastases, indicating correlation morphological characteristics. PRDX4 identified as highly expressed both selected for further characterization. Our revealed that “Prdx4” (PrxIV, AOE372) shows functional similarities other Prx...
This White Paper is authored by 11 industry real-world evidence (RWE) experts, with support from IQVIA, as part of the 'RWE Leadership Forum': a group leaders who come together noncompetitive partners to understand and respond internal or external RWD/E challenges opportunities single expert voice. Herein we aim clarify rules engagement between pharma healthcare in order establish trust-based partnerships, which will unlock unique value for society, including medical community ultimate...
The article Bridging the Gap Between RCTs and RWE Through Endpoint Selection, written by Robert J. LoCasale, Chris L. Pashos, Ben Gutierrez, Nancy A. Dreyer, Toby Collins, Alan Calleja, Michael Seewald, Jonathan M. Plumb, Johan Liwing, Maurille Feudjo Tepie, Sajan Khosla, was originally published electronically on publisher's internet portal 06 July 2020 without open access. With author(s)' decision to opt for Open Choice copyright of changed 01 September © Author(s) is forthwith distributed...
The past decade has seen the increasing influence and relevance of real-world data (RWD) evidence (RWE) in healthcare decision making. value added by RWD/RWE prompted pharmaceutical industry to develop high performing systems practices harness power generated at global level. However, this worldwide transformation provides outstanding opportunities support capability building within local affiliates impact key country-level stakeholders through resulting evidence. Therefore, we present an...