A5001451399- Gut microbiota and health
- Probiotics and Fermented Foods
- Reproductive tract infections research
- Diet and metabolism studies
- Dermatology and Skin Diseases
- Clostridium difficile and Clostridium perfringens research
- Gastrointestinal motility and disorders
- Bacterial biofilms and quorum sensing
- Urinary Tract Infections Management
- Allergic Rhinitis and Sensitization
- Genomics and Phylogenetic Studies
- Fungal Biology and Applications
- Bacteriophages and microbial interactions
- Infant Nutrition and Health
- Plant Reproductive Biology
- Bacterial Genetics and Biotechnology
- Fungal and yeast genetics research
- Cervical Cancer and HPV Research
- Mycorrhizal Fungi and Plant Interactions
- Plant Molecular Biology Research
- Sex work and related issues
- Digestive system and related health
- Food Allergy and Anaphylaxis Research
- Biosensors and Analytical Detection
- Microbial Metabolites in Food Biotechnology
Netherlands Organisation for Applied Scientific Research
2013-2024
Leiden University
2021
Pfizer (United States)
2018
Pension Fund for Care and Well-Being
2005-2015
Academic Center for Dentistry Amsterdam
2008
University of Amsterdam
2008
Marine Biological Laboratory
2008
Vrije Universiteit Amsterdam
2008
Food & Nutrition
2001-2006
Netherlands Organisation for Health Research and Development
2005
Development of non-alcoholic fatty liver disease (NAFLD) is linked to obesity, adipose tissue inflammation, and gut dysfunction, all which depend on diet. So far, studies have mainly focused diet-related fecal microbiota changes, but other compartments may be more informative host health. We present a first systematic analysis changes in the ileum colon using multiple diets investigating both mucosal samples. Ldlr−/−.Leiden mice received one three different energy-dense (ED)-diets (n =...
A good definition of commensal microflora and an understanding its relation to health are essential in preventing combating disease. We hypothesized that the species richness human oral is underestimated. Saliva supragingival plaque were sampled from 71 98 healthy adults, respectively. Amplicons V6 hypervariable region small-subunit ribosomal RNA gene generated by PCR, pooled into saliva pools, sequenced means Genome Sequencer 20 system at 454 Life Sciences. Data evaluated taxonomic...
Lactobacillus plantarum is a versatile and flexible species that encountered in variety of niches can utilize broad range fermentable carbon sources. To assess if this versatility linked to variable gene pool, microarrays containing subset small genomic fragments L. strain WCFS1 were used perform stringent genotyping 20 strains from various The categories with the most genes conserved all those involved biosynthesis or degradation structural compounds like proteins, lipids, DNA. Conversely,...
Enterococcus faecium, an ubiquous colonizer of humans and animals, has evolved in the last 15 years from avirulent commensal to third most frequently isolated nosocomial pathogen among intensive care unit patients United States. E. faecium combines multidrug resistance with potential horizontal gene transfer even more pathogenic bacteria. Little is known about evolution virulence genomic studies are hampered by absence a completely annotated genome sequence. To further unravel its evolution,...
Recently, the concept of prebiotics has been revisited to expand beyond non-digestible oligosaccharides, and requirements for selective stimulation were extended include microbial groups other than, additional to, bifidobacteria lactobacilli. Here, gut microbiota-modulating effects well-known novel studied. An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed different dietary fibers that had a range concentrations (inulin, alpha-linked...
Human milk oligosaccharides (HMO) and prebiotic are proposed to confer several health benefits the infant. They shape microbiota, have anti-inflammatory properties, support epithelial barrier functioning. However, in order select best for inclusion infant formulas, there is a need increase our understanding of specific effects HMO prebiotics on host immune system. Therefore, we investigated sialyllactose (SL), galactooligosaccharides (GOS) functioning, microbiota composition, SCFA...
A vaginal microbiota dominated by lactobacilli (particularly Lactobacillus crispatus) is associated with health, whereas a not considered dysbiotic. Here we investigated whether L. crispatus strains isolated from the tract of women Lactobacillus-dominated (LVM) are pheno- or genotypically distinct samples dysbiotic (DVM). We studied 33 (n = 16 LVM; n 17 DVM). Comparison these two groups showed that, although strain differences existed, both degraded various carbohydrates, produced similar...
The organic acid lactate is the predominant fermentation product of Lactobacillus plantarum. undissociated form this a strong growth inhibitor for organism. Different theories have been postulated to explain inhibitory effects lactic acid: (i) toxicity arising from dissipation membrane potential, (ii) acidification cytosol, or (iii) intracellular anion accumulation. In general, stresses are complex study, since their highly dependent on degree dissociation and thus pH. transcription profiles...
Background Isolates of the Enterobacter cloacae complex have been increasingly isolated as nosocomial pathogens, but phenotypic identification E. is unreliable and irreproducible. Identification species based on currently available genotyping tools already superior to identification, taxonomy isolates belonging this cumbersome. Methodology/Principal Findings This study shows that multilocus sequence analysis comparative genomic hybridization a mixed genome array powerful method for studying...
Bacillus subtilis forms dormant spores upon nutrient depletion. Under favorable environmental conditions, the spore breaks its dormancy and resumes growth in a process called germination outgrowth. To elucidate physiological processes that occur during transition of to an actively growing vegetative cell, we studied this time-dependent manner by combination microscopy, analysis extracellular metabolites, genome-wide transcription. The results indicate presence abundant levels late...
Although their exact function remains enigmatic, bifidobacteria are among the first colonizers of newborn infant gut and further develop into abundant communities, notably in response to diet. Therefore, transcriptional responses rapidly processed fecal samples from young infants that were fed either breast milk or a formula containing mixture galacto- fructo-oligosaccharides studied. The presence diversity bifidobacterial communities determined using PCR-denaturing gradient gel...
To date, women are most often diagnosed with bacterial vaginosis (BV) using microscopy based Nugent scoring or Amsel criteria. However, the accuracy is less than optimal. The aim of present study was to confirm identity known BV-associated composition profiles and evaluate indicators for BV three molecular methods.Evaluation carried out by 16S rRNA amplicon sequencing V5-V7 region, a tailor-made oligonucleotide-based microarray, PCR-based profiling technique termed IS-profiling, which on...
In this study, we collected water from different locations in 32 drinking distribution networks the Netherlands and analysed spatial temporal variation microbial community composition by high-throughput sequencing of 16S rRNA gene amplicons. We observed that compositions raw source processed were very for each network sampled. network, major differences between water, although structures did not differ substantially end-point tap water. End-point samples within same revealed similar...
Prebiotics are considered to have potential reduce disturbances in the gut microbiota induced by antibiotics. Results literature are, however, not consistent. The current vitro study conducted a fermentation screening platform allowed unambiguously compare impact of galacto-oligosaccharides (GOS) on adult composition and activity upon treatment with four antibiotics at two doses. changes relative abundance bacteria antibiotic growth Bifidobacterium Lactobacillus GOS addition were dose...
Exposure to microbes may be important in the development of atopic disease. Atopic diseases have been associated with specific characteristics intestinal microbiome. The link between microbiota and food allergy has rarely studied, gold standard for diagnosing (double-blind placebo-controlled challenge [DBPCFC]) seldom used. We aimed distinguish fecal microbial signatures children dermatitis (AD).Pediatric patients AD, without allergy, were included this cross-sectional observational pilot...