A5003730068- Infant Nutrition and Health
- Asthma and respiratory diseases
- Gut microbiota and health
- Pediatric health and respiratory diseases
- Neonatal Respiratory Health Research
- IL-33, ST2, and ILC Pathways
- Immune Cell Function and Interaction
- Probiotics and Fermented Foods
- Immune Response and Inflammation
- Parasites and Host Interactions
- Digestive system and related health
- Viral gastroenteritis research and epidemiology
- Biopolymer Synthesis and Applications
- Neonatal Health and Biochemistry
- Clostridium difficile and Clostridium perfringens research
- Clinical Nutrition and Gastroenterology
- Animal health and immunology
- Tryptophan and brain disorders
- Immunotherapy and Immune Responses
- Proteins in Food Systems
- Dermatology and Skin Diseases
- Diabetes and associated disorders
- Biochemical Analysis and Sensing Techniques
- Cancer Research and Treatments
- Child Nutrition and Feeding Issues
Monash University
2019-2024
The Alfred Hospital
2022-2024
Monash Alfred Psychiatry Research centre
2023
Wageningen University & Research
2015-2019
Bipar
2018
Stockholm University
2013
Barrier integrity is central to the maintenance of healthy immunological homeostasis. Impaired skin barrier function linked with enhanced allergen sensitization and development diseases such as atopic dermatitis (AD), which can precede other allergic disorders, for example, food allergies asthma. Epidemiological evidence indicates that children suffering from have lower levels dietary fibre-derived short-chain fatty acids (SCFA). Using an experimental model AD-like inflammation, we report a...
Human milk oligosaccharides (HMO) and prebiotic are proposed to confer several health benefits the infant. They shape microbiota, have anti-inflammatory properties, support epithelial barrier functioning. However, in order select best for inclusion infant formulas, there is a need increase our understanding of specific effects HMO prebiotics on host immune system. Therefore, we investigated sialyllactose (SL), galactooligosaccharides (GOS) functioning, microbiota composition, SCFA...
Scope Investigations into the immunological response of proteins is often masked by lipopolysaccharide (LPS) contamination. We report an optimized Triton X-114 (TX-114) based LPS extraction method for β-lactoglobulin (BLG) and soy protein extract suitable cell-based assays. Methods results Optimization existing TX-114 phase resulted in >99% reduction levels. However, remaining was found to interfere with concentration assays decreased viability THP-1 macrophages HEK-Blue 293 cells. Upon...
Soil-transmitted helminths cause widespread disease, infecting ~1.5 billion people living within poverty-stricken regions of tropical and subtropical countries. As adult worms inhabit the intestine alongside bacterial communities, we determined whether microbiota impacted on host resistance against intestinal helminth infection. We infected germ-free, antibiotic-treated specific pathogen-free mice, with Heligmosomoides polygyrus bakeri. Mice harboured increased parasite numbers in absence a...
Abstract With aging, tryptophan metabolism is affected. Tryptophan has a crucial role in the induction of immune tolerance and maintenance gut microbiota. We, therefore, studied effect dietary restriction young wild-type (WT) mice (118-wk life span) DNA-repair deficient, premature-aged (Ercc1−/Δ7) (20-wk span). First, we found that aging on distribution B T cells bone marrow (BM) periphery 16-wk-old Ercc1−/Δ7 was comparable to 18-mo-old WT mice. Dietary caused an arrest cell development BM,...
The human milk oligosaccharide 3′-sialyllactose (3′SL) has previously been shown to activate murine dendritic cells (DC) in a Toll-like receptor (TLR) 4-mediated manner ex vivo. In this study we aimed investigate whether 3′SL similar immunomodulatory properties on DC. was induce NF-κB activation via TLR4. However, LPS detected the commercially obtained from different suppliers. After removal of 3′SL, studied its ability modify DC differentiation vitro. contrast and LPS-free did not...
Ageing is associated with a changing immune system, leading to inflammageing (increased levels of inflammation markers in serum) and immunosenescence (reduced cells reduced responses towards pathogens). This results vaccination increased infections elderly. Much known about the adaptive system upon ageing, but less innate system. Therefore, aim this study was compare function Toll like receptor (TLR)-mediated between elderly young adult women. To end, women were compared effect ageing on...
Lactoferrin is an abundant glycoprotein in bovine milk that has immunomodulatory effects on human cells. Bovine lactoferrin (LF) binds lipopolysaccharides (LPS) with high affinity and postulated to act via TLR4-dependent -independent mechanisms. It been shown LF modulates differentiation of monocytes into tolerogenic dendritic However, a previous study, we showed LPS also mediates cells (DC). Since affinity, it remains be investigated whether or mediating these effects. We, therefore,...
During aging the immune system is dysregulated. Especially plasmacytoid dendritic cells (pDCs) and myeloid DCs (mDCs) have reduced Toll like receptor (TLR)-mediated responses resulting in increased susceptibility to infections. Consumption of bovine lactoferrin (bLF) has been shown reduce infections with viruses. Galacto-oligosacharides (GOS) vitamin D are associated pro-inflammatory cytokine levels serum, TLR7/8 responses, respectively. A double-blind placebo-controlled nutritional...
Breast milk plays an important role in immune development early life and protects against diseases later life. A wide range of the beneficial effects breast are attributed to human oligosaccharides (HMOs) as well components such vitamin D3 (VitD3) or TGFβ. One mechanism by which HMOs might contribute homeostasis protection disease is induction a local tolerogenic milieu. In this study we investigated effect 6'-sialyllactose (6'SL) 2'-fucosyllactose (2'FL) prebiotic galactooligosaccharides...
The Fulani are known to have a lower parasitaemia and less clinical episodes of malaria as compared the Dogon sympatric ethnic group, living in Mali. Higher circulating malaria-specific antibody titers increased pro-inflammatory cytokine levels been shown individuals. Several studies tried link haptoglobin (Hp) phenotypes with susceptibility malaria, but without consensus. This study investigated role Hp during asymptomatic Plasmodium falciparum infection.Two different cohorts were combined...
Intestinal helminth infection triggers a type 2 immune response that promotes ‘weep-and sweep’ characterised by increased mucus secretion and intestinal hypermotility, which function to dislodge the worm from its habitat. Recent studies have discovered several other pathogens cause dysmotility through major alterations enteric nervous systems (ENS), their interactions, within gastrointestinal tract. However, involvement of these has not been investigated for infections. Eosinophils represent...
Abstract Intestinal helminth infection triggers a type 2 immune response that promotes ‘weep-and sweep’ characterised by increased mucus secretion and intestinal hypermotility, which function to dislodge the worm from its habitat. Recent studies have discovered several other pathogens cause dysmotility through major alterations enteric nervous systems (ENS), their interactions, within gastrointestinal tract. However, involvement of these has not been investigated for infections. Eosinophils...
<b>P</b>-cresol sulfate (PCS) is a microbial metabolite derived from L-tyrosine and was recently discovered to have immunoregulatory influences on allergic airway inflammation. Administering PCS mice reduced house dust mite-induced CCL20 production, chemokine that recruits lymphocytes dendritic cells (Wypych et al. <i>Nature Immunology</i> 2021). We are using as a chemical template develop novel therapeutics against asthma. Our aim determine the molecular mechanism of action its...