A5014114512- Asthma and respiratory diseases
- Pediatric health and respiratory diseases
- Neonatal Respiratory Health Research
- Gut microbiota and health
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Traumatic Brain Injury and Neurovascular Disturbances
- Neonatal and fetal brain pathology
- Diet and metabolism studies
- Intensive Care Unit Cognitive Disorders
- Cystic Fibrosis Research Advances
- Sphingolipid Metabolism and Signaling
- Pediatric Pain Management Techniques
- Respiratory and Cough-Related Research
- Helicobacter pylori-related gastroenterology studies
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Dermatology and Skin Diseases
- Tracheal and airway disorders
- Allergic Rhinitis and Sensitization
- Sepsis Diagnosis and Treatment
- Inhalation and Respiratory Drug Delivery
- Renal function and acid-base balance
- Medical Imaging and Pathology Studies
- Respiratory Support and Mechanisms
- Smoking Behavior and Cessation
- Infant Health and Development
Monash University
2020-2025
The University of Adelaide
2019-2022
Royal Adelaide Hospital
2019-2020
Wenzhou Medical University
2019
First Affiliated Hospital of Wenzhou Medical University
2019
Early-life microbial colonization of the skin may modulate immune system and impact development atopic dermatitis (AD) allergic diseases later in life. To address this question, we assessed association between microbiome AD, barrier integrity first year We further explored evolution with age its possible determinants, including delivery mode.
Background and aim In cystic fibrosis, gastrointestinal dysfunction lower airway infection occur early are independently associated with poorer outcomes in childhood. This study aimed to define the relationship between microbiota at each niche during first 2 years of life, its association growth inflammation, explanatory features metabolome. Materials methods 67 bronchoalveolar lavage fluid (BALF), 62 plasma 105 stool samples were collected from 39 infants fibrosis 0 24 months who treated...
Pneumonia is a common comorbidity in patients with severe traumatic brain injury (TBI), and associated increased morbidity mortality. In this study, we established model of intratracheal Klebsiella pneumoniae administration young adult male female mice, at 4 days following an experimental TBI, to investigate how K. infection influences acute post-TBI outcomes. A dose-response curve determined the optimal dose for inoculation (1 x 10^6 colony forming units), resulted transient body weight...
There is increasing evidence that the airway microbiome plays a key role in establishment of respiratory health by interacting with developing immune system early life. While it has become clear bacteria are involved this process, there knowledge gap concerning fungi. Moreover, inter-kingdom interactions influence development remain unknown. In prospective exploratory human study, we aimed to determine post-natal microbial and immunological features upper airways 121 healthy newborns.
Persistent post-surgical pain (PPSP) occurs in a proportion of patients following surgical interventions. Research suggests that specific microbiome components are important for brain development and function, with recent studies demonstrating chronic results changes to the microbiome. Consumption high fat, sugar (HFHS) diet can drastically alter composition is modifiable risk factor many neuroinflammatory conditions. Therefore, we investigated how daily consumption HFHS modified PPSP,...
ABSTRACT Background and objective E‐cigarettes are often marketed thought of as emitting harmless vapour; however, verification their safety for non‐smokers is scarce. We have previously shown that cause decreased phagocytosis bacteria by macrophages via reductions in surface bacterial recognition receptors. This study assessed the effect E‐cigarette constituents, 3 E‐liquid apple flavours, nicotine, vegetable glycerine propylene glycol, on bronchial epithelial cell viability, apoptosis...
Abstract In chronic obstructive pulmonary disease (COPD) apoptotic bronchial epithelial cells are increased, and their phagocytosis by alveolar macrophages (AM) is decreased alongside bacterial phagocytosis. Epithelial cellular lipids, including those exposed on uncleared bodies, can become oxidized, may be recognized presented as non-self antigen presenting cells. CD1b a lipid-presenting protein, previously only described in dendritic We investigated whether upregulated COPD AM, lipid...
Wheezing in childhood is prevalent, with over one-half of all children experiencing at least 1 episode by age 6. The pathophysiology wheeze, especially why some develop asthma while others do not, remains unclear. This study addresses the knowledge gap investigating transition from preschool wheeze to using multiomic profiling. Unsupervised, group-agnostic integrative factor analysis was performed host/bacterial (meta)transcriptomic and bacterial shotgun metagenomic datasets bronchial brush...
Patients with a traumatic brain injury (TBI) are susceptible to hospital-acquired infections, presenting significant challenge an already-compromised immune system. The consequences and mechanisms by which this dual insult worsens outcomes poorly understood. This study aimed explore how systemic stimulus (lipopolysaccharide, LPS) influences following experimental TBI in young adult mice. Male female C57Bl/6J mice underwent controlled cortical impact or sham surgery, followed 1 mg/kg i.p. LPS...
Adolescent chronic pain is a growing public health epidemic. Our understanding of its etiology limited; however, several factors can increase susceptibility, often developing in response to an acute trigger such as surgical procedure or mild traumatic brain injury (mTBI), adverse childhood experience (ACE). Additionally, the prevalence and manifestation sexually dimorphic, with double rates females than males. Despite this, majority pre-clinical research focuses on males, leaving gap...
Abstract Objectives Idiopathic pulmonary fibrosis (IPF) is a devastating progressive interstitial lung disease with poor outcomes. While decades of research have shed light on pathophysiological mechanisms associated the disease, our understanding early molecular events driving IPF and its progression limited. With this study, we aimed to model leading edge using data‐driven approach. Methods Multiple omics modalities (transcriptomics, metabolomics lipidomics) healthy explants representing...
Cigarette smoke (CS)-induced emphysema is an important contributor to chronic obstructive pulmonary disease (COPD). We have shown the efficacy of azithromycin in reducing airway inflammation COPD and exacerbations severe asthma; however, effects long-term on development not been shown. employed live animal imaging monitor emphysema-like interventional treatment CS-exposed mice. BALB/c mice (female, 10 weeks; n = 10) were exposed CS for 1 hr twice daily, 5 days/week, 12 weeks (CS). Half...
Abstract Aim Protracted bacterial bronchitis (PBB) is considered a potential precursor to bronchiectasis (BE) in some children. We previously showed that alveolar macrophages (AM) from children with PBB or BE have similar significant defect phagocytic capacity, proinflammatory associations. hypothesized the mechanisms responsible for this involve dysregulation of sphingosine‐1‐phosphate (S1P) signaling pathway, as we found adult inflammatory lung diseases. Method employed Custom TaqMan...
Inflammasomes and sphingosine-1-phosphate (S1P) signalling are increasingly subject to intensive research in human diseases. We hypothesize that respiratory muco-obstructive diseases, mucus obstruction enhances NLRP3 inflammasome activation dysregulated S1P signalling.Lung tissues from mice overexpressing the beta-unit of epithelial sodium channel (βENaC) their littermate controls were examined by histology, immunofluorescence confocal microscopy, followed ImageJ quantitative analysis.Lower...
Differentiated air-liquid interface models are the current standard to assess mucociliary phenotype using clinically-derived samples in a controlled environment. However, obtaining basal progenitor airway epithelial cells (AEC) from lungs is invasive and resource-intensive. Hence, we applied tissue engineering approach generate organotypic sinonasal AEC (nAEC) epithelia determine whether they predictive of bronchial (bAEC) models. Basal were isolated healthy participants cytological brushing...
<b>P</b>-cresol sulfate (PCS) is a microbial metabolite derived from L-tyrosine and was recently discovered to have immunoregulatory influences on allergic airway inflammation. Administering PCS mice reduced house dust mite-induced CCL20 production, chemokine that recruits lymphocytes dendritic cells (Wypych et al. <i>Nature Immunology</i> 2021). We are using as a chemical template develop novel therapeutics against asthma. Our aim determine the molecular mechanism of action its...
with antibiotics: Meropenem 1 gram/day, Ciprofloxacin 400 mg/day Fosfomycin 2 gram/day.Meropenem was given by intravenous electric syringe pump over approximately 3 hours.Ciprofloxacin infusion one time a day.Fosformycin diluted glucose 5% and 15 minutes every 48 hours after haemodialysis.When we administered Fosfomicin but within the same line (Figure B), saw precipitation in line.At once, stopped close monitored him.He still stable.Then, put two distinct lines for drug.In conclusion, shows...