A5061308933- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- RNA and protein synthesis mechanisms
- Chemical Synthesis and Analysis
- RNA Interference and Gene Delivery
- Neuroendocrine regulation and behavior
- Advanced biosensing and bioanalysis techniques
- Antimicrobial Peptides and Activities
- DNA and Nucleic Acid Chemistry
- Biochemical and Structural Characterization
- Peptidase Inhibition and Analysis
- RNA modifications and cancer
- RNA Research and Splicing
- Click Chemistry and Applications
- Chemical Reaction Mechanisms
- Insect Resistance and Genetics
- Chromosomal and Genetic Variations
- Signaling Pathways in Disease
- HIV Research and Treatment
- Pain Mechanisms and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Plant biochemistry and biosynthesis
- Hypothalamic control of reproductive hormones
- Supramolecular Self-Assembly in Materials
- Bacteriophages and microbial interactions
University of Gdańsk
2011-2024
Huta Stalowa Wola (Poland)
2004
Gdańsk Medical University
2004
Kyoto Pharmaceutical University
2004
Gdańsk University of Technology
1997-2002
North Carolina State University
2001
St. Josef-Hospital
1998
Institute of Physics
1995
Max Planck Institute for Medical Research
1995
Adam Mickiewicz University in Poznań
1995
Abstract In the presented study, transportan 10 (TP10), an amphipathic cell penetrating peptide (CPP) with high translocation activity, was conjugated vancomycin (Van), which is known for poor access to intracellular bacteria and brain. The antibacterial activity of conjugates tested on selected clinical strains methicillin-resistant Staphylococcus aureus (MRSA) Enterococcus sp . It turned out that all them had superior antimicrobial in comparison free Van, became visible particularly...
Parkinson's disease (PD) is a common progressive neurodegenerative disorder for which the current treatment not fully satisfactory. One of major drawbacks PD therapy poor penetration drugs across blood-brain barrier (BBB). In recent years, cell-penetrating peptides (CPPs) such as Tat, SynB, or TP10 have gained great interest due to their ability penetrate cell membranes and deliver different cargos targets including central nervous system (CNS). However, there no data with respect use CPPs...
Introduction. Cell penetrating peptides (CPPs) have the ability to translocate through cell membranes with high efficiency and therefore can introduce biological agents pharmaceutical properties into cell. Transportan (TP) its shorter analog transportan 10 (TP10) are among best studied CPPs, however, their effects on viability of cargo introduction colorectal cancer (CRC) cells yet not been investigated. The aim our study was evaluate cytotoxic TP TP10 representative CRC lines protein...
The aim of this paper was to examine whether cell-penetrating peptides (CPPs) such as transportan 10 (TP10) or protein transduction domain (PTD4) may improve the anticancer activity cisplatin (cPt). complexes TP10 PTD4 with cPt were used in experiments. They carried out on two non-cancer (HEK293 (human embryonic kidney) and HEL299 embryo lung)) cancer (HeLa cervical cancer) OS143B osteosarcoma 143B)) cell lines. Both tested (MTT assay) respect their cytotoxic actions. TAMRA (fluorescent...
Proprotein convertase subtilisin/kexin 9 (PCSK9) is a protein that plays key role in the metabolism of low-density lipoprotein (LDL) cholesterol. The gain-of-function mutations PCSK9 gene lead to reduced number surface LDL receptors by binding them, eventually leading endosomal degradation. This, turn, culprit hypercholesterolemia, resulting accelerated atherogenesis. modern treatment for hypercholesterolemia encompasses use biological drugs against PCSK9, like monoclonal antibodies and...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of arginine-vasopressins, modified in positions 1 and 2, as antagonists the vasopressor response to parent hormoneBernard Lammek, Piotr Rekowski, Gotfryd Kupryszewski, Per Melin, Ulf RagnarssonCite this: J. Med. Chem. 1988, 31, 3, 603–606Publication Date (Print):March 1, 1988Publication History Published online1 May 2002Published inissue March...
The sequence-dependent, acid- or base-catalysed aspartimide formation is one of the most serious side reactions in solid-phase synthesis peptides containing aspartic acid. In present work, we investigated susceptibility 4-(N-[1-(4,4-dimethyl-2,6-dioxocyclohexylidene)-3-methylbutyl]amino)benzyl (Dmab), an acid beta-carboxy side-chain protecting group, for formation. As a model, 15-amino acid-residue galanin fragment analogue Asp-Ala motif was used during Fmoc-based synthesis. Our study showed...
BackgroundMost studies on regenerative medicine focus cell-based therapies and transplantations. Small-molecule therapeutics, though proved effective in different medical conditions, have not been extensively investigated research. It is known that healing potential decreases with development developmental changes are driven by epigenetic mechanisms, which suggests repression of capacity.MethodsWe applied zebularine, a nucleoside inhibitor DNA methyltransferases, to stimulate the response...
The objective of this study was to design and synthesize a new CPP-PNA conjugate that would be able penetrate endothelial cells, bind STAT1 mRNA thereby block the activity (the Signal Transducer Activator Transcription 1), which is important in cases vessel inflammation. In course study, TAMRA-PTD-4- Hal(traziole-Gly-PNA)-conjugate successfully synthesized using specific 1,3-dipolar Huisgen cycloaddition reaction known as "click reaction". hybridization properties complementary hSTAT1 ssDNA...
Aspartimide (Asi) formation is one of the most serious side reactions that can occur both during solid phase synthesis and storage peptides containing aspartic acid. Although numerous studies on mechanism Asi conducted so far, problem still remains unresolved relatively little known about impact this reaction biological properties such modified peptides. In present work we characterized effect galanin(1-15) analogue in position 14 with acid, investigating its action rat isolated gastric...
The Cu(I) catalyzed Huisgen 1,3-dipolar azide-alkyne cycloaddition (CuAAC) was applied for a nucleoside-peptide bioconjugation. Systemin (Sys), an 18-aa plant signaling peptide naturally produced in response to wounding or pathogen attack, chemically synthesized as its N-propynoic acid functionalized analog (Prp-Sys) using the SPPS. Next, CuAAC conjugate Prp-Sys with 3′-azido-2′,3′-dideoxythymidine (AZT), model cargo molecule. 1,4-Linked 1,2,3-triazole AZT-Sys designed characterize spreading...
Nucleopeptides (NPs) represent synthetic polymers created by attaching nucleobases to the side chains of amino acid residues within peptides. These compounds amalgamate characteristics peptides and nucleic acids, showcasing a unique ability recognize RNA structures. In this study, we present design synthesis Fmoc-protected nucleobase acids (1,4-TzlNBAs) new class NPs, where canonical are affixed chain L-homoalanine (Hal) through 1,4-linked-1,2,3-triazole (HalTzl). 1,4-TzlNBAs suitable for...
We describe the synthesis and pharmacological properties of two series analogues: one which consists three peptides having L‐1‐naphthylalanine in position 3 second composed analogues substituted with L‐2‐naphthylalanine. All were tested bioassays for pressor antidiuretic activities. also checked uterotonic activity vitro . observed that counterparts both is, cases, strikingly different. One of. new analogues, [(L‐2‐Nal) , (D‐Arg) 8 ]VP is among most potent antagonists vasopressor response to...
The contributions of the natural modified nucleosides to RNA identity in protein/RNA interactions are not understood. We had demonstrated that 15 amino acid long peptides could be selected from a random phage display library using criterion binding modified, rather than unmodified, anticodon domain yeast tRNAPhe (ASLPhe). Affinity and specificity for ASLPhe have been characterized by fluorescence spectroscopy peptides' tryptophans. One selected, peptide tF2, exhibited highest most...
HERV-W is a multi-locus family of human endogenous retroviruses (HERVs) that has been found to play an important role in physiology and pathology. Two particular members are special interests: ERVWE1 (coding syncytin-1, which glycoprotein essential the formation placenta) MSRV (multiple sclerosis-associated retrovirus thought significant pathology as result its increased expression brain tissue blood cells derived from patients with multiple sclerosis (MS)). Both mRNA share high level...
Arginine-rich cell-penetrating peptides (RRCPPs) exhibit intrinsic neuroprotective effects on neurons injured by acute ischemic stroke. Conformational properties, interaction, and the ability to penetrate neural membrane are critical for of RRCCPs. In this study, we applied circular dichroism (CD) spectroscopy coarse-grained molecular dynamics (CG MD) simulations investigate interactions two RRCPPs, Tat(49–57)-NH2 (arginine-rich motif Tat HIV-1 protein) PTD4 (a less basic Ala-scan analog...
Abstract Systemin (Sys) is an 18‐aa plant peptide hormone involved in the regulation of plant's defensive response. Sys considered as a fast‐spreading systemic wound signal. We developed simple and rapid CE method to monitor spreading peptides through tomato plant. A 1,2,3‐triazole‐linked AZT‐systemin conjugate was designed model study possibility translocating small cargo molecules 3'‐Azido‐2',3'‐dideoxythymidine by systemin. The (Sys, N ‐propiolyl Sys, conjugate) were injected into stem...