A5075225554- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- DNA and Nucleic Acid Chemistry
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Genomics and Phylogenetic Studies
- Photosynthetic Processes and Mechanisms
- Botanical Research and Chemistry
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- Plant Molecular Biology Research
- Toxin Mechanisms and Immunotoxins
- MicroRNA in disease regulation
- Plant Disease Resistance and Genetics
- Plant Genetic and Mutation Studies
- Protein Structure and Dynamics
- Bacteriophages and microbial interactions
- Enzyme Structure and Function
- Epigenetics and DNA Methylation
- Bacterial Genetics and Biotechnology
- Plant Stress Responses and Tolerance
- RNA regulation and disease
- Chemical Synthesis and Analysis
- Analytical Chemistry and Chromatography
Institute of Bioorganic Chemistry, Polish Academy of Sciences
2015-2024
Polish Academy of Sciences
2007-2024
Adam Mickiewicz University in Poznań
1971-2024
Centrum Badań Molekularnych i Makromolekularnych Polskiej Akademii Nauk
2019
Poznan University of Medical Sciences
2016
Freie Universität Berlin
2000-2012
Centre National de la Recherche Scientifique
1982-2011
Institut de Biologie Moléculaire des Plantes
2011
Université de Strasbourg
2011
Oregon State University
2011
Malignant gliomas represent the most devastating group of brain tumors in adults, among which glioblastoma multiforme (GBM) exhibits highest malignancy rate. Despite combined modality treatment, GBM recurs and is invariably fatal. A further insight into molecular background gliomagenesis required to improve patient outcomes. The primary aim this study was gain broad information on miRNA expression pattern malignant gliomas, mainly GBM. We investigated global profile glioma tissues with...
Group II introns are large catalytic RNAs that ancestrally related to nuclear spliceosomal introns. Sequences corresponding group found in many prokaryotes and particularly prevalent within plants organellar genomes. Proteins encoded the themselves (maturases) facilitate splicing of their own host pre-RNAs. Mitochondrial have diverged considerably sequence lost maturases. In angiosperms, only a single maturase has been retained mitochondrial DNA: matR gene NADH dehydrogenase 1 (nad1) intron...
Background and Aims Epigenetic regulation plays an important role in the management of plant growth, development response to stress factors, several reports have indicated that DNA methylation a critical seed viability. This study examines changes 5-methylcytosine (m5C) levels seeds during ageing, process has implications for conservation agriculture. Methods Changes global level m5C were measured mature oak, Quercus robur. The extent was using protocol based on two-dimensional thin-layer...
Ribosomal 5S RNA (5S rRNA) is the ubiquitous component found in large subunit of ribosomes all known organisms. Due to its small size, abundance and evolutionary conservation rRNA for many years now used as a model molecule studies on structure, RNA–protein interactions molecular phylogeny. 5SRNAdb (http://combio.pl/5srnadb/) first database that provides high quality reference set ribosomal RNAs across three domains life. Here, we give an overview new developments associated web tools since...
Micro RNAs (miRNAs) (19-25 nucleotides in length) belong to the group of non-coding are most abundant posttranscriptional regulators multicellular organisms. They affect a gene expression by binding fully or partially complementary sequences 3'-UTR target mRNA. Furthermore, miRNAs present mechanism which genes with diverse functions on multiple pathways can be simultaneously regulated at post-transcriptional level. However, little is known about specific through sequence structural motifs...
The effects of storage and deep desiccation on structural changes DNA in orthodox seeds are poorly characterized. In this study we analyzed the 5-methylcytosine (m5C) global content isolated from common pear (Pyrus communis L.) that had been subjected to extreme desiccation, seedlings derived these seeds. Germination seedling emergence tests were applied determine seed viability after their desiccation. parallel, analysis m5C dried obtained such was performed with a 2D TLC method....
The generally accepted model of the miRNA-guided RNA down-regulation suggests that mature miRNA targets mRNA in a nucleotide sequence-specific manner. However, we have shown sequence is not only determinant specificity. Using specific nucleases, T1, V1 and S1 as well NMR, UV/Vis CD spectroscopies, found miR-21, miR-93 miR-296 can adopt hairpin and/or homoduplex structures. secondary structure those miRNAs solution function concentration ionic conditions. Additionally, formation facilitated...
Glioblastoma multiforme (GBM) is the most common type of malignant gliomas, characterized by genetic instability, intratumoral histopathological variability and unpredictable clinical behavior. Disappointing results in treatment gliomas with surgery, radiation chemotherapy have fueled a search for new therapeutic targets modalities. Here we report approach towards RNA interference therapy glioblastoma based on magnetic nanoparticles delivery double-stranded (dsRNA) homological sequences to...
In last few years much data has accumulated which shows that in different cells various RNA transcripts are synthesized. They lack protein coding capacity and do not produce mature protein. It seems they work mainly or exclusively on the level. Their function mechanism of action is poorly understood. this paper we have collected all known transcript prepared a database for further structural functional studies. This first collection nucleotide sequences RNAs kind. The can be accessed via WWW...
In contrast to the current view that kinetin (N6-furfuryladenine) is an unnatural and synthetic compound, we have detected it in commercially available DNA, freshly extracted cellular DNA from human cells plant cell extracts by two independent methods. First, discovered N6-furfuryladenine has electrochemical properties can be applied for monitoring this modified base a HPLC/UV/EC method. Second, confirmed assignments mass-spectrometric analysis. A pathway of formation proposed which furfural...
Recently, we have reported the presence of kinetin (N6-furfuryladenine) in commercially available DNA, freshly extracted cellular DNA and plant cell extracts. We also found that has electrochemical properties which can be used for monitoring level this modified base DNA. Here, first time, propose a mechanism formation vivo, based on analyses its mass spectra. Since hydroxy radical oxidation at carbon 5' deoxyribose residue yields furfural, aldehyde reacts with amino group adenine and, after...
The noncoding RNA database (ncRNAdb) was created as a source of information on molecules, which do not possess protein-coding capacity. It is now widely accepted that, in addition to constitutively expressed, housekeeping or infrastructural RNAs, there wide variety RNAs participating mechanisms involved regulation gene expression at all levels transmission genetic from DNA proteins. Noncoding RNAs' activities include chromatin structure remodeling, transcriptional and translational...
Glioblastoma multiforme (GBM) is the most common type of malignant gliomas, characterized by genetic instability, intratumoral histopathological variability and unpredictable clinical behaviour. Disappointing results in treatment gliomas with surgery, radiation chemotherapy have fuelled a search for new modalities. Malignant express preferentially number surface markers that may be exploited as therapeutic targets, such tenascin-C (TN-C), an extracellular matrix glycoprotein contributes to...