Background and Aims Epigenetic regulation plays an important role in the management of plant growth, development response to stress factors, several reports have indicated that DNA methylation a critical seed viability. This study examines changes 5-methylcytosine (m5C) levels seeds during ageing, process has implications for conservation agriculture. Methods Changes global level m5C were measured mature oak, Quercus robur. The extent was using protocol based on two-dimensional thin-layer...

Poor storability of recalcitrant seeds is due to their inability tolerate low moisture content. Understanding the processes underlying recalcitrance a prerequisite developing maintenance strategy and prolonging lifespan. Multiple studies have investigated differences between orthodox (desiccation-tolerant) (desiccation-sensitive) seeds. Information on epigenetic regulation, however, lacking thus limits our understanding defining physiology In present comparative study, changes in global...

Modifications of DNA nucleobases are present in all forms life. The purpose these modifications eukaryotic cells, however, is not always clear. Although the role 5-methylcytosine (m5C) epigenetic regulation and maintenance stability plant genomes becoming better understood, knowledge pertaining to origin function oxidized still scarce. formation 5-hydroxymetylcytosine (hm5C) especially debatable. modifications, functioning as regulatory factors or serving injury markers, may have an effect...

Abstract We here present the spontaneously immortalised cell line, HaSKpw, as a novel model for multistep process of skin carcinogenesis. HaSKpw cells were established from epidermis normal human adult that, without crisis, are now growing unrestricted and feeder-independent. At passage 22, clonal populations clone7 (HaSKpwC7) was further compared to also immortalized HaCaT cells. As important differences, express wild-type p53, remain pseudodiploid, show unique chromosomal profile with...

Ageing and deterioration of seeds is a major problem for the maintenance seed quality viability during long-term storage. Prediction early stages in order to point out plantlets' regeneration time challenge successful In preserved seeds, damages accumulate within cells at rate mainly related their moisture content temperature Current research reveals global alterations DNA methylation lipid-rich intermediate desiccation storage various regimes covering nonoptimal optimal conditions. We show...

The level of 5-methylcytosine (m5C) in DNA has been observed to change plants response biotic and abiotic stress factors. Little information reported on alterations methylation orthodox tree seeds storage conditions. In the current study, epigenetic integrity was analyzed Pyrus communis L. conventional cryogenic storage. results indicate that under optimal conditions resulted a significant increase m5C. contrast, decrease m5C after cryostorage at high water content (WC) observed, not only...

Ex situ preservation of genetic resources is an essential strategy for the conservation plant biodiversity. In this regard, seed storage most convenient and efficient way preserving germplasm future breeding efforts. A better understanding molecular changes that occur during desiccation aging necessary to improve protocols, as well real-time methods monitoring quality. present study, we assessed in level genomic 5-methylcytosine (5mC) seeds Populus nigra L. by 2D-TLC. Epigenetic were...

The preservation of the nuclear genome's integrity is paramount for viability and overall health cells, tissues, organisms. DNA, being susceptible to damage under physiological conditions vulnerable both endogenous environmental factors, faces constant threats. To assess DNA repair within individual eukaryotic comet assay presents itself as a versatile, gel electrophoresis-based, relatively simple, highly sensitive method. Originally designed monitor populations mammalian has now found...

All seeds eventually die even under optimal storage conditions. The moment of viability loss is difficult to predict and detect. In order differentiate between dead viable dormant orthodox seeds, GC-MS analysis was used non-invasively evaluate the volatile signature Pyrus communis L. Sorbus aucuparia Dormant are capable extended metabolic depression. However, their low rate remains largely unquantified, there no measurements metabolites, i.e. organic compounds (VOC) for physiologically...

Most plants encounter water stress at one or more different stages of their life cycle. The maintenance genetic stability is the integral component desiccation tolerance that defines storage ability and long-term survival seeds. Embryonic axes desiccation-sensitive recalcitrant seeds Acer pseudoplatnus L. were used to investigate genotoxic effect desiccation. Alkaline single-cell gel electrophoresis (comet assay) methodology was optimized provide unique insights into onset repair DNA strand...

Abstract To date, the use of liquid nitrogen (LN) in plant gene banks is relatively limited. Globally, 2021, approximately 10,000 accessions were cryopreserved, and their explants derived from vitro plants. In Europe, out 500 banks, only 20 cryogenic temperatures to store germplasms. The implementation seven conservation projects Poland starting 1990s meant that banking system this country began gradually develop. Plant bank mainly based on collection seeds makes little tissues, including...

Abstract A variable response of Prunus avium L. seeds to desiccation and storage in liquid nitrogen (LN) has been reported the literature. The majority these experiments were conducted on initially dried seeds. LN exposure tolerance fresh P. is unknown. In present study, used determine seed cryopreservation. Desiccation from a moisture content (MC) 19.7-20.2% 10.1-10.9% or 07.6-8.5% reduced seedling emergence approximately 73 19 16% for first provenance; 89 10-12% second provenance After LN,...

<ja:p>English yew( L.) is a strictly outcrossing and dioecious species whose populations are small isolated. It known that sex ratios may vary in natural due to local environmental conditions or stochastic events. However, unbalanced have negative impacts on genetic diversity through enhanced drift inbreeding. The present study represents one of the first attempts compare variation at microsatellite loci within between with different gender proportions. Our results indicated there were no...

Abstract The problems posed by seed sensitivity to desiccation and aging have motivated the development of various techniques for mitigating their detrimental effects. redox priming seeds in antioxidant solution improve postharvest performance is one approaches. Spermidine (Spd) was tested as an invigorating on nondormant recalcitrant (desiccation-sensitive) silver maple (Acer saccharinum L.). treatment resulted 8–10% increase germination capacity subjected mild severe desiccation, while...

To understand the importance of terrestrial solar exposure on human skin, not only individual spectral components need to be considered in biomedical studies, but also relevance combined action profile complete spectrum (cSS) must established. We therefore developed a novel irradiation device that combines emission four lamps (UVB, UVA, VIS and nIR) achieve from 280 1400 nm with controllable lamps. The integrated irradiance each band is similar spectrum. can utilised individually or any...

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