A5101786517- Seaweed-derived Bioactive Compounds
- Microbial Natural Products and Biosynthesis
- Essential Oils and Antimicrobial Activity
- Marine Sponges and Natural Products
- Tea Polyphenols and Effects
- Tannin, Tannase and Anticancer Activities
- Fungal Biology and Applications
- Nutrition, Genetics, and Disease
- Carcinogens and Genotoxicity Assessment
- Echinoderm biology and ecology
- Antioxidant Activity and Oxidative Stress
- Algal biology and biofuel production
- Phytochemicals and Antioxidant Activities
- Free Radicals and Antioxidants
- Diet and metabolism studies
- DNA Repair Mechanisms
- Bioactive Compounds and Antitumor Agents
- Environmental Toxicology and Ecotoxicology
- Pharmaceutical and Antibiotic Environmental Impacts
- Natural product bioactivities and synthesis
- Synthesis of Organic Compounds
- X-ray Diffraction in Crystallography
- 3D Printing in Biomedical Research
- Crystallization and Solubility Studies
- Seed Germination and Physiology
Universidade do Porto
2014-2024
Centro Interdisciplinar de Investigação Marinha e Ambiental
2018-2019
University of Colorado Anschutz Medical Campus
2017
University of Minho
2008-2015
University of Oslo
2010-2013
Czech Academy of Sciences, Biology Centre
2008
Colorectal cancer therapy with 5-fluorouracil (5-Fu) frequently become ineffective due to resistance this drug; and thus other effective compounds are essential for therapy. It is well-known marine brown seaweeds contain antioxidant the carotenoid fucoxanthin (Fx) polyphenolic compound phloroglucinol (Ph) which exerted diverse biological activities including anticancer. The aim of study was determine anticancer Fx or Ph alone as well combination each chemical 5-Fu on two human colorectal...
A previously unreported bis-indolyl benzenoid, candidusin D (2e) and a new hydroxypyrrolidine alkaloid, preussin C (5b) were isolated together with fourteen described compounds: palmitic acid, clionasterol, ergosterol 5,8-endoperoxides, chrysophanic acid (1a), emodin (1b), six benzenoids including asterriquinol dimethyl ether (2a), petromurin (2b), kumbicin B (2c), (2d), 2″-oxoasterriquinol methyl (3), (4), the alkaloid (5a), (3S, 6S)-3,6-dibenzylpiperazine-2,5-dione (6) 4-(acetylamino)...
Salvia officinalis (common sage) is a plant with antidiabetic properties. A pilot trial (non-randomized crossover trial) six healthy female volunteers (aged 40-50) was designed to evaluate the beneficial properties of sage tea consumption on blood glucose regulation, lipid profile and transaminase activity in humans. Effects erythrocytes' SOD CAT activities Hsp70 expression lymphocytes were also evaluated. Four weeks treatment had no effects plasma glucose. An improvement observed lower LDL...
DNA damage can lead to carcinogenesis if replication proceeds without proper repair. This study evaluated the effects of water extracts three Salvia sp., officinalis (SO), fruticosa (SF), and lavandulifolia (SL), major phenolic constituents, rosmarinic acid (RA) luteolin-7-glucoside (L-7-G), on protection in Caco-2 HeLa cells exposed oxidative agents repair cells. The comet assay was used measure capacity. final concentration each sage extract 50 μg/mL, concentrations RA L-7-G were 20 μM,...
Salvia officinalis and some of its isolated compounds have been found to be preventive DNA damage increased proliferation in vitro colon cells. In the present study, we used azoxymethane model test effects S. on cancer prevention vivo. The results showed that sage treatment reduced number ACF formed only if administered before injection, demonstrating tea drinking has a chemopreventive effect colorectal cancer. A decrease marker Ki67 H2O2-induced azoxymethane-induced colonocytes lymphocytes...
Compounds isolated from the marine sea fan-derived fungus Neosartorya siamensis (KUFA 0017), namely, 2,4-dihydroxy-3-methylacetophenon (1), chevalone C (2), nortryptoquivaline (4), tryptoquivaline H (6), F (7), fiscalin A (8), epi-fiscalin (9), epi-neofiscalin (11) and (13) were tested for anti-proliferative activity by MTT assay, DNA damage induction comet of cell death nuclear condensation assay on colon HCT116, liver HepG2 melanoma A375 cancer lines. 2, 4, 8, 9, 11 13 presented IC50...
Colorectal cancer (CRC) is one of the most frequently occurring carcinomas which require effective therapies. Fucosterol a sterol present in marine brown seaweeds with several biological activities. However, influence fucosterol CRC remains to be determined. Thus, aim this study was examine anticancer activity alone and combination 5-fluorouracil (5-Fu) on two human cell lines (HCT116 HT29) compared cytotoxicity normal colon fibroblast line (CCD-18co) monolayer (2D). The effect or 5-Fu...
Preussin, a hydroxyl pyrrolidine derivative isolated from the marine sponge-associated fungus Aspergillus candidus KUFA 0062, displayed anticancer effects in some cancer cell lines, including MCF7. Preussin was investigated for its cytotoxic and antiproliferative breast lines (MCF7, SKBR3, MDA-MB-231), representatives of major cancers subtypes, non-tumor line (MCF12A). first tested 2D (monolayer), then 3D (multicellular aggregates), cultures, using multi-endpoint approach cytotoxicity...
DNA damage induced by oxidative and alkylating agents contributes to carcinogenesis, leading possible mutations if replication proceeds without proper repair. However, some are used in cancer therapy due their ability induce subsequently apoptosis of tumor cells. In this study, the genotoxic effects hydrogen peroxide (H2O2) N-methyl-N-nitrosourea (MNU) 1,3-bis-(2-chloroethyl)-1-nitosourea (BCNU) were examined two colon cell lines (HCT15 CO115). was assessed comet assay with lesion-specific...
To evaluate the in vitro anticancer activity of crude ethyl acetate extracts culture four marine-derived fungi Aspergillus similanensis KUFA 0013 (E1), Neosartorya paulistensis KUFC 7897 (E2), siamensis 0017 (E4) and Talaromyces trachyspermus 0021 (E3) on a panel seven human cancer cell lines. Effects proliferation, induction DNA damage death were assessed by MTT clonogenic assays, comet assay nuclear condensation assay, respectively. The proliferation HepG2, HCT116 A375 cells decreased...
Seaweed bioactive compounds have shown anticancer activities in vitro and vivo studies. However, tests remain limited, with conflicting results, effects combination drugs are even scarcer. Here, the cytotoxic of five seaweed (astaxanthin, fucoidan, fucosterol, laminarin, phloroglucinol) were tested alone (cisplatin—Cis; doxorubicin—Dox), breast cell lines (three cancer (BC) subtypes one non-tumoral). The combinations revealed situations where presented potentiation or inhibition drugs’...
Fucoxanthin (Fx) is a carotenoid derived from marine organisms that exhibits anticancer activities. However, its role as potential drug adjuvant in breast cancer (BC) treatment still poorly explored. Firstly, this study investigated the cytotoxic effects of Fx alone and combined with doxorubicin (Dox) cisplatin (Cis) on panel 2D-cultured BC cell lines (MCF7, SKBR3 MDA-MB-231) one non-tumoral line (MCF12A). induced cytotoxicity against all potentiated Dox towards MDA-MB-231 cells. The...
Three-dimensional cell cultures may better mimic avascular tumors. Yet, they still lack characterization and standardization. Therefore, this study aimed to (a) generate multicellular aggregates (MCAs) of four breast lines: MCF7, MDA-MB-231, SKBR3 (tumoral) MCF12A (non-tumoral) using ultra-low attachment (ULA) plates, (b) detail the methodology used for their formation analysis, providing technical tips, (c) characterize MCAs morphometry, qualitative cytology (at light electron microscopy),...
Pharmacognosy Research,2016,8,1,8-15.DOI:10.4103/0974-8490.171105Published:December 2015Type:Original ArticleAuthors:Alice Abreu Ramos, Bruno Castro-Carvalho, Maria Prata-Sena, Tida Dethoup, Suradet Buttachon, Anake Kijjoa, and Eduardo Rocha Author(s) affiliations:Alice Ramos1, Castro-Carvalho2, Prata-Sena2, Dethoup3, Buttachon4, Kijjoa4, Rocha2 1Interdisciplinary Center for Marine Environmental Research, University of Porto, PORTUGAL. 2Interdisciplinary Porto; Department Microscopy,...
Drug resistance is a major concern in the current chemotherapeutic approaches and combination with natural compounds may enhance cytotoxic effects of anticancer drugs. Therefore, this study evaluated cytotoxicity crude ethyl extracts six marine-derived fungi - Neosartorya tsunodae KUFC 9213 (E1), laciniosa 7896 (E2), fischeri 6344 (E3), Aspergillus similanensis KUFA 0013 (E4), paulistensis 7894 (E5), Talaromyces trachyspermum 0021 (E6) when combined doxorubicin (Dox), seven human cancer cell...
Sudden hearing loss (SHL) is an ENT emergency defined as sensorineural (SNHL) ≥ 30 dB HL affecting at least 3 consecutive tonal frequencies, showing a sudden onset, and occurring within days. In cases of SHL, detailed investigation should be performed in order to determine the etiology provide best treatment. Otoacoustic emission (OAE) analysis, electronystagmography (ENG), bithermal caloric test (BCT), vestibular evoked myogenic potential (VEMP) assessments may used addition number...
Most plants encounter water stress at one or more different stages of their life cycle. The maintenance genetic stability is the integral component desiccation tolerance that defines storage ability and long-term survival seeds. Embryonic axes desiccation-sensitive recalcitrant seeds Acer pseudoplatnus L. were used to investigate genotoxic effect desiccation. Alkaline single-cell gel electrophoresis (comet assay) methodology was optimized provide unique insights into onset repair DNA strand...