A5037998137- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Neuroscience and Neuropharmacology Research
- Antimicrobial Peptides and Activities
- Marine Sponges and Natural Products
- Microbial Natural Products and Biosynthesis
- Ion channel regulation and function
- Salmonella and Campylobacter epidemiology
- Reproductive tract infections research
- Tryptophan and brain disorders
- Pediatric health and respiratory diseases
- Crystallography and molecular interactions
- Neurogenesis and neuroplasticity mechanisms
- Adipose Tissue and Metabolism
- Nitric Oxide and Endothelin Effects
- Viral gastroenteritis research and epidemiology
- Stress Responses and Cortisol
- Genetics and Neurodevelopmental Disorders
- Bryophyte Studies and Records
- Natural Compound Pharmacology Studies
- Escherichia coli research studies
- Animal Nutrition and Physiology
- HIV/AIDS Research and Interventions
- Sulfur Compounds in Biology
- Synthetic Organic Chemistry Methods
University of Coimbra
2007-2024
Universidade do Porto
2018-2020
Centro Interdisciplinar de Investigação Marinha e Ambiental
2017-2018
Institute of Cell Biology and Neurobiology
2012
Centro de Neurociências e Biologia Celular
2012
Background The need for woman-controlled, cheap, safe, effective, easy-to-use and easy-to-store topical applications prophylaxis against sexually transmitted infections (STIs) makes surfactant-containing formulations an interesting option that requires a more fundamental knowledge concerning surfactant toxicology structure-activity relationships. Methodology/Principal Findings We report in vitro effects of concentration, exposure time structure on the viability mammalian cell types typically...
Broad-spectrum antimicrobial activity of quaternary ammonium surfactants (QAS) makes them attractive and cheap topical prophylactic options for sexually transmitted infections perinatal vertically urogenital infections. Although attributed to their high affinity biological membranes, the mechanisms behind QAS microbicidal are not fully understood. We evaluated how structure affects whether this can be exploited use in prophylaxis bacterial infections.Acute toxicity vitro models human...
Surfactants have long been known to microbicidal action and extensively used as antiseptics disinfectants for a variety of general hygiene clinical purposes. Among surfactants, quaternary ammonium compounds (QAC) are be the most useful disinfectants. However, our previous toxicological studies showed that QAC also toxic surfactants mammalian cells. An understanding mechanisms underlie toxicity is crucial first step in their rational use design development more effective safer molecules. We...
A previously unreported bis-indolyl benzenoid, candidusin D (2e) and a new hydroxypyrrolidine alkaloid, preussin C (5b) were isolated together with fourteen described compounds: palmitic acid, clionasterol, ergosterol 5,8-endoperoxides, chrysophanic acid (1a), emodin (1b), six benzenoids including asterriquinol dimethyl ether (2a), petromurin (2b), kumbicin B (2c), (2d), 2″-oxoasterriquinol methyl (3), (4), the alkaloid (5a), (3S, 6S)-3,6-dibenzylpiperazine-2,5-dione (6) 4-(acetylamino)...
Abstract Nitric oxide (NO) was described to inhibit the proliferation of neural stem cells. Some evidence suggests that NO, under certain conditions, can also promote cell proliferation, although mechanisms responsible for a potential proliferative effect NO in cells have remained unaddressed. In this work, we investigated and characterized cultures obtained from mouse subventricular zone. We found donor NOC-18 (10 μM) increased whereas higher concentrations (100 inhibited proliferation....
A previously unreported dihydrochromone dimer, paecilin E (1), was isolated, together with eleven known compounds: β-sitostenone, ergosta-4,6,8 (14), 22-tetraen-3-one, cyathisterone, byssochlamic acid, dehydromevalonic acid lactone, chevalone B, aszonalenin, dankasterone (2), helvolic secalonic and fellutanine A, from the culture filtrate extract of marine sponge-associated fungus Neosartorya fennelliae KUFA 0811. Nine reported metabolites, including a chromanol derivative (3), (3β, 5α,...
Due to the emergence of multidrug-resistant pathogenic microorganisms, search for new antimicrobial compounds plays an important role in current medicinal chemistry research. Inspired by lichen xanthones, a series novel chlorinated xanthones was prepared using five chlorination methods (Methods A–E) obtain different patterns substitution xanthone scaffold. All synthesized were evaluated their activity. Among them, 3-chloro-4,6-dimethoxy-1-methyl-9H-xanthen-9-one 15 showed promising...
Proteolytic cleavage of the Na+/Ca2+ exchanger (NCX) by calpains impairs calcium homeostasis, leading to a delayed overload and excitotoxic cell death. However, it is not known whether reversal contributes activate trigger neuronal We investigated role NCX in Ca2+ dynamics, calpain activation viability, α-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) receptor-stimulated hippocampal neurons. Selective overactivation AMPA receptors caused NCX, which accounted for approximately 30% rise...
Abstract Mutations linked to neurodevelopmental disorders, such as intellectual disability (ID), are frequently found in genes that encode for proteins of the excitatory synapse. Transmembrane AMPA receptor regulatory (TARPs) auxiliary regulate crucial aspects function. Here, we investigate a mutant form TARP family member stargazin, described an ID patient. Molecular dynamics analyses predicted ID-associated stargazin variant, V143L, weakens overall interface AMPAR:stargazin complex and...
Nitric oxide (NO) is an important inflammatory mediator involved in the initial boost proliferation of neural stem cells following brain injury. However, mechanisms underlying proliferative effect NO are still unclear. The aim this work was to investigate whether cyclic GMP (cGMP) and cGMP-dependent kinase (PKG) triggered by cells. For purpose, cultures isolated from mouse subventricular zone (SVZ) were used. We observed that long-term exposure donor (24 h), NOC-18, increased SVZ a manner,...
Quaternary ammonium compounds (QAC) are widely used, cheap, and chemically stable disinfectants topical antiseptics with wide-spectrum antimicrobial activities. Within this group of compounds, we recently showed that there significant differences between the pharmacodynamics n-alkyl quaternary surfactants (QAS) a short (C12) alkyl chain when in vitro toxicities toward bacterial mammalian epithelial cells compared. These result an attractive therapeutic window justifies studying short-chain...
As filter-feeding animals farmed in water bodies exposed to anthropogenic influences, oysters can be both useful bioremediators and high-risk foodstuffs, considering that they are typically consumed raw. Understanding the dynamic of bacterial viral load Pacific oyster (Crassostrea gigas) tissues, hemolymph, outer shell surface biofilm, farming is therefore great importance for microbiological risk assessment. A one-year survey collected from a class B production area (Canal de Mira, on...
Abstract The aberrant formation and function of neuronal synapses are recognized as major phenotypes in many cases neurodevelopmental (NDDs) -psychiatric disorders (NPDs). A growing body research has identified an expanding number susceptibility genes encoding proteins with synaptic function. Here, we present the first brain-focused characterization a potential new gene, ARHAGP8 , which encodes Rho GTPase activating protein (RhoGAP). Accumulating evidence suggests that ARHGAP8 plays pivotal...
Summary The M-current is a low-threshold potassium current that modulates neuronal excitability and suppresses repetitive firing. However, the mechanisms regulating M-channel function remain unclear. We identified type I Transmembrane AMPA receptor Regulatory Proteins (TARPs) as Kv7.2 subunit interactors in cortical neurons, with their interaction increasing upon depolarization. Co-expression of TARPs increased channel surface expression Kv7.2-mediated currents, while disrupting TARP-γ2...
<title>Abstract</title> Chronic stress exerts profound negative effects on cognitive and emotional behaviours is a major risk factor for the development of neuropsychiatric disorders. However, molecular links between chronic its deleterious neuronal synaptic function remain elusive. Here, using combination <italic>in vitro</italic> vivo</italic> approaches, we demonstrate that upregulation miR-186-5p triggered by may be key mediator such changes, leading to dysfunction. Our results show...
1. In this study, classical and molecular microbiological methods for detection quantification of Campylobacter spp. were used to estimate their prevalence in faecal samples skin swabs collected from 31 broiler flocks (20 farms) Portugal, measure the impact transport-related factors on expected rising excretion rates farm slaughterhouse. 2. Data husbandry practices transport conditions gathered, including time transit, distance travelled or ante-mortem plant-holding time. 3. A generalised...
Antimicrobial resistance is one of the most pressing health issues our days. The marine environment has proven to be a very rich source diverse natural products with broad-spectra biologically activities being helpful resource in search for novel antimicrobial compounds. These structurally distinct molecules are revealing promising biological against large number drug-resistant pathogenic bacteria and fungi, catching attention discovery new agents. Inspired by lichen xanthones [1]...