A5006309645- Pain Mechanisms and Treatments
- Botulinum Toxin and Related Neurological Disorders
- Congenital Diaphragmatic Hernia Studies
- Neonatal Respiratory Health Research
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Nerve injury and regeneration
- Neurological and metabolic disorders
- Natural Compound Pharmacology Studies
- Synthesis and biological activity
- Pancreatitis Pathology and Treatment
- Inflammation biomarkers and pathways
- Parkinson's Disease Mechanisms and Treatments
- Heart Failure Treatment and Management
- Congenital Anomalies and Fetal Surgery
- Inflammasome and immune disorders
- Calpain Protease Function and Regulation
- Axon Guidance and Neuronal Signaling
- Biological Activity of Diterpenoids and Biflavonoids
- Acupuncture Treatment Research Studies
- Neuroscience of respiration and sleep
- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- Vitamin K Research Studies
- Alcoholism and Thiamine Deficiency
Universidade do Porto
2011-2025
Centro Interdisciplinar de Investigação Marinha e Ambiental
2018-2020
Institute of Biomedical Science
2020
Children's Hospital Research Institute of Manitoba
2015-2017
University of Manitoba
2015-2017
University of Minho
2015
Objective and Background: Our objective was to determine the fetal in vivo microRNA signature hypoplastic lungs of human fetuses with severe isolated congenital diaphragmatic hernia (CDH) changes tracheal amniotic fluid undergoing fetoscopic endoluminal occlusion (FETO) reverse lung hypoplasia due CDH. Methods: We profiled expression prenatal by microarray analysis. then validated this real-time quantitative polymerase chain reaction CDH patients FETO. further explored role miR-200b using...
neuronal hyperactivity at the spinal cord during mechanical hyperalgesia induced by diabetes may result from a decrease in local expression of potassium chloride co-transporter 2 (KCC2), which shifts action neurotransmitter γ-amminobutiric acid (GABA) inhibitory to excitatory. In this study, we evaluated effects microglia inhibition or brain-derived neurotrophic factor (BDNF) blockade on KCC2 expression, activity and mechanically pain responses streptozotocin (STZ)-diabetic rats.four weeks...
Objective: We aimed to evaluate the use of miR-200b as a prenatal transplacental therapy in nitrofen rat model abnormal lung development and congenital diaphragmatic hernia (CDH). Background: Pulmonary hypoplasia (PH) pulmonary hypertension determine mortality morbidity CDH babies. There is no safe medical treatment available. previously discovered that higher associated with better survival Here, we investigate role PH its an vivo therapy. Methods: profiled expression during...
Abstract Background and aims Endocan has been scarcely explored in COVID-19, especially regarding its modulation by veno-venous extracorporeal membrane oxygenation (VV-ECMO), hypertension or previous renin–angiotensin–aldosterone system (RAAS) inhibitors treatment. We compared endocan other endotheliitis markers hospitalized COVID-19 patients assessed their VV-ECMO, RAAS Material methods Serum endocan, intercellular adhesion molecule-1 (ICAM-1), vascular cell (VCAM-1) E-selectin were...
Abstract Painful diabetic neuropathy may be due to impairments in descending modulation of nociceptive transmission at the spinal cord. In present study, streptozotocin rats (STZ rats) with neuropathic symptoms (mechanical hypersensitivity) were used perform a time‐course evaluation neuronal activity dorsal horn and periaqueductal grey matter (PAG), major brainstem area pain modulation. The expression Fos protein, marker activation, progressively increased 4 10 weeks. At PAG, increases...
Due to the emergence of multidrug-resistant pathogenic microorganisms, search for new antimicrobial compounds plays an important role in current medicinal chemistry research. Inspired by lichen xanthones, a series novel chlorinated xanthones was prepared using five chlorination methods (Methods A–E) obtain different patterns substitution xanthone scaffold. All synthesized were evaluated their activity. Among them, 3-chloro-4,6-dimethoxy-1-methyl-9H-xanthen-9-one 15 showed promising...
A series of thirteen xanthones 3–15 was prepared based on substitutional (appendage) diversity reactions. The structurally characterized their spectral data and HRMS, the structures xanthone derivatives 1, 7, 8 were determined by single-crystal X-ray diffraction. This series, along with an in-house aminated 16–33, tested for in-vitro antimicrobial activity against seven bacterial (including two multidrug-resistant) strains five fungal strains. 1-(Dibromomethyl)-3,4-dimethoxy-9H-xanthen-9-one...
Key points Retinoic acid (RA) and ghrelin levels are altered in human hypoplastic lungs when compared to healthy lungs. Although considerable data have been obtained about RA, bombesin the congenital diaphragmatic hernia (CDH) rat model, neuroendocrine factors never associated with RA signalling pathway this animal model. In study, interaction between was explored CDH The authors found that normal fetal lung explants treated showed an increase growth. Hypoplastic presented higher expression...
Cysteinyl leukotrienes (CysLT) are potent inflammation-promoting mediators, but remain scarcely explored in COVID-19. We evaluated urinary CysLT (U-CysLT) relationship with disease severity and their usefulness for prognostication hospitalized COVID-19 patients. The impact on U-CysLT of veno-venous extracorporeal membrane oxygenation (VV-ECMO) comorbidities such as hypertension obesity was also assessed.Blood spot urine were collected "severe" (n = 26), "critically ill" 17) ill VV-ECMO"...
Background/ Aims: The knowledge of the molecular network that governs fetal lung branching is an essential step towards discovery novel therapeutic targets against pulmonary pathologies. Lung consists two highly branched systems: airways and vasculature. Ephrins its receptors, Eph, have been implicated in cardiovascular development, angiogenesis vascular remodeling. This study aims to clarify role these factors during morphogenesis. Methods: Ephrins-B1, -B2 receptor EphB4 expression pattern...
Aims: Inflammation-driven endothelitis seems to be a hallmark of acute heart failure (AHF) and cardiogenic shock (CS). Endocan, soluble proteoglycan secreted by the activated endothelium, contributes inflammation endothelial dysfunction, but has been scarcely explored in human AHF. We aimed evaluate serum (S-Endocan) urinary endocan (U-Endocan) profiles AHF CS patients correlate them with biomarkers/parameters inflammation, activation, cardiovascular dysfunction prognosis. Methods: Blood...
Aim: To evaluate the effects of antioxidant treatment streptozotocin (STZ)-diabetic rats with α-lipoic acid (α-LA) in neuronal and microglial activation at spinal cord, an important relay station nociceptive transmission. Because role potassium chloride co-transporter 2 (KCC2) cord influence microglia KCC2 expression, we also evaluated α-LA expression cord. Methods: Four weeks after STZ injection, received daily intraperitoneal injections (100 mg/kg), during weeks. Mechanical nociception was...
Due to the emergence of multidrug-resistant pathogenic microorganisms, search for novel antimicrobials is urgent. Inspired by marine alkaloids, a series indolomethyl pyrazino [1,2-b]quinazoline-3,6-diones was prepared using one-pot microwave-assisted multicomponent polycondensation amino acids. The compounds were evaluated their antimicrobial activity against panel nine bacterial strains and five fungal strains. Compounds 26 27 most effective Staphylococcus aureus ATCC 29213 reference strain...
Diabetes is a major health problem with an alarming increasing prevalence, and the most frequent cause of neuropathy worldwide. Neuropathy affects 50–60% diabetic patients, being life-quality impairment for quarter these patients. Diabetic neuropathic pain (DNP) characterized by spontaneous pain, mechanical hyperalgesia tactile allodynia accompanied functional neurochemical changes at peripheral nerves, spinal cord supraspinal control areas. Regarding effects in central somatossensory...
Cysteinyl leukotrienes (CysLT) are potent vascular leakage-promoting agents but have been scarcely explored in human septic shock (SS). We evaluated CysLT at admission and during hospitalization their correlation with endothelial dysfunction, inflammation, oxidative stress, the renin–angiotensin–aldosterone system, cardiac, renal, respiratory, hepatic parameters SS patients. Blood spot-urine samples were collected days 1–2 (admission), 3–4, 5–8 patients (n = 13) a single time point controls...