A5082159212- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Fungal Biology and Applications
- Retinal Diseases and Treatments
- Signaling Pathways in Disease
- Peptidase Inhibition and Analysis
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Asthma and respiratory diseases
- Nail Diseases and Treatments
- Angiogenesis and VEGF in Cancer
- Ovarian function and disorders
- Microbial Natural Products and Biosynthesis
- Ubiquitin and proteasome pathways
- Preterm Birth and Chorioamnionitis
- Cystic Fibrosis Research Advances
- Antimicrobial agents and applications
- Infectious Diseases and Mycology
- Cell Adhesion Molecules Research
- Neuroblastoma Research and Treatments
- Retinal Development and Disorders
- Retinal Imaging and Analysis
- Laser Applications in Dentistry and Medicine
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Probiotics and Fermented Foods
- Testicular diseases and treatments
Dartmouth College
2023-2025
The University of Texas at San Antonio
2016-2020
Texas Center for Infectious Disease
2018-2020
ABSTRACT Contemporary antifungal therapies utilized to treat filamentous fungal infections are inhibited by intrinsic and emerging drug resistance. Consequently, there is an urgent need develop novel compounds that effective against drug-resistant fungi. Here, we Aspergillus fumigatus cell-based high-throughput screen identify small molecules with activity also potentiated triazole activity. The identified 16 hits promising A. . A nonspirocyclic piperidine, herein named MBX-7591, exhibited...
Mucor circinelloides is a fungus that belongs to the order Mucorales. It grows as mold in environment and can cause mucormycosis, potentially fatal infection immunocompromised patients. M. biodiesel producer serves model organism for studying several biological processes, such light responses RNA interference-mediated gene silencing. Over past decade, increasing number of molecular tools has also allowed us manipulate genome this fungus. This article outlines fundamental protocols vitro...
Mucor is intrinsically resistant to most known antifungals, which makes mucormycosis treatment challenging. Calcineurin a serine/threonine phosphatase that widely conserved across eukaryotes. When calcineurin function inhibited in , growth shifts less virulent yeast form, an attractive target for development of new antifungal drugs. Previously, we identified two distinct mechanisms through can become inhibitors involving Mendelian mutations the gene FKBP12, including corresponding A or B...
Infections triggered by pathogenic fungi cause a serious threat to the public health care system. In particular, an increase of antifungal drug-resistant has resulted in difficulty treatment. A limited variety drugs available treat patients left us situation where we need develop new therapeutic approaches that are less prone development resistance fungi. this study, demonstrate efficacy nanoemulsion NB-201, which utilizes surfactant benzalkonium chloride, against human-pathogenic We found...
Mucor circinelloides, a dimorphic opportunistic pathogen, expresses three heterotrimeric G-protein beta subunits (Gpb1, Gpb2 and Gpb3). The Gpb1-encoding gene is up-regulated during mycelial growth compared with that in the spore or yeast stage. gpb1 deletion mutation analysis revealed its relevance for an adequate development transition hyphal under low oxygen concentrations. Infection assays mice indicated phenotype considerably reduced virulence tissue invasiveness mutants (Δgpb1)...
Angiogenesis mediated by proteins such as Fibroblast Growth Factor-2 (FGF-2) is a vital component of normal physiological processes and has also been implicated in contributing to the disease state associated with various microbial infections. Previous studies our group others have shown that Candida albicans, common agent candidiasis, induces FGF-2 secretion vitro angiogenesis brains kidneys during systemic However, underlying mechanism(s) via which fungus increases production role(s)...
Diabetic retinopathy is manifested by excessive angiogenesis and high level of vascular endothelial growth factor (VEGF) in the eye.Human (MIO-M1) rat (rMC-1) Müller cells were treated with 0, 5.5, or 30mM glucose for 24 hours. Viable cell counts obtained Trypan Blue Dye Exclusion Method. ELISA was used to determine VEGF levels medium.Compared hour treatment 5.5mM glucose, MIO-M1 rMC-1 increased viable number 38% 24% respectively. In contrast, 0mM decreased 28% 50% Compared 5.5mM, had medium...
Previous studies have shown that in diabetic patients, there is an increase of retinal capillaries associated with the development retinopathy eye. The objective current study to investigate effect glucose on endothelial cell viability and VEGF secretion. 20,000 cells per well were treated without or 5.5mM (euglycemic), 18.5mM 30mM (hyperglycemic) for 24 hours. Viable counted using Trypan blue dye exclusion method. ELISA was used measure secretion from into medium. number viable incubated...
Angiogenesis mediated by proteins such as Fibroblast Growth Factor – 2 (FGF-2) is a vital component of normal physiological processes and has also been implicated in contributing to disease state associated with various microbial infections. Previous studies our group others have shown that Candida albicans, common agent candidiasis, induces FGF-2 expression vitro, angiogenesis brains kidneys during systemic However, the underlying mechanism(s) via which fungus increases role(s)...
Angioinvasion and tissue injury are hallmarks of infections mediated by Mucor circinelloides (mucormycosis) Candida albicans (systemic candidiasis).Several studies have shown that pro-angiogenic Fibroblast Growth Factor-2 (FGF-2) is essential for repair.Recently, FGF-2 treatment increased survival rates murine models aspergillosis.However, the role its regulated angiogenesis understudied in systemic candidiasis mucormycosis infections.C. a dimorphic fungus exhibits yeast hyphae...
Abstract The emergence of immunocompromising diseases such as HIV/AIDS or other immunosuppressive medical conditions have opened an opportunity for fungal infections to afflict patients globally. An increase antifungal drug resistant fungi posed a serious threat patients. Combining these circumstances with limited variety drugs available treat has left us in situation where we need develop new therapeutic approaches that are less prone development resistance by pathogenic fungi. In this...
Abstract Mucormycosis is an emerging lethal fungal infection in immunocompromised patients. Mucor circinelloides a causal agent of mucormycosis and serves as model system to understand genetics Mucorales. Calcineurin conserved virulence factor many pathogenic fungi calcineurin inhibition or deletion the regulatory subunit (CnbR) results shift from hyphal yeast growth. We analyzed thirty-six inhibitor resistant bypass mutants that exhibited growth presence inhibitors yeast-locked cnbR Δ...