An efficient ultrasound-assisted extraction technique was employed to extract total flavonoids from Sparganii rhizoma. The optimum conditions for the highest yield of were ethanol concentration 53.62%, ultrasonication time 29.41 min and ultrasound power 300 W, which determined using response surface methodology. yields optimal higher than conventional extraction. crude then purified on a polyamide resin, whereby content in increased 94.62%. antioxidant activities including DPPH radical...

This study investigated the antioxidant potential and anti-fatigue effects of phenolics extracted from seed coat Euryale ferox Salisb. The in vitro potentials, including scavenging DPPH, hydroxyl radical activities reducing power were evaluated. Antioxidant status vivo was analyzed by SOD, CAT, GSH-Px MDA content liver kidneys D-galactose-induced aging mice. effect evaluated using an exhaustive swimming test, along with determination LDH, BUN HG content. phenolic extract possessed notable on...

A novel method was developed for the simultaneous determination of β2-agonist residues such as terbutaline, salbutamol, and clenbuterol by high-performance liquid chromatography (HPLC) coupled with chemiluminescence (CL) detection. The procedure based on enhancement effect β2-agonists CL reaction between luminol complex trivalent copper periodate ([Cu(HIO6)2]5-), which on-line electrogenerated constant current electrolysis. HPLC separation used a Nucleosil RP-C18 column (250 mm × 4.6 i.d., 5...

The effects of purity tea polysaccharides (TPS) on its five antioxidant activities and hypoglycemic in vitro were studied. results showed that the higher TPS, lower capacity. FTPSI is highest (sugar content 80.72%), but than those Fujian (FTPS) FTPSII. activity polysaccharide related to protein polyphenol (Pearson r > .90). protective effect Zhejiang FTPS human umbilical vein endothelial cells (HUVEC) was better purified fractions. inhibition rates FTPSII (5 2 mg/ml) α-glucosidase (32.76%)...

The present study investigated the potential hypoglycemic and antioxidant effects of polysaccharides extracted from petioles pedicels Euryale ferox Salisb. (EFPP) on alloxan-induced hyperglycemic mice. EFPP had a total carbohydrate 65.72 ± 2.81%, uronic acid 4.56 0.62% protein 0.58 0.12%, with an average molecular weight 1.02 kDa to 11.45 monosaccharide composition Man, GlcA, Rha, Glc, Gal Ara at molar ratio 0.12 : 0.01 9.57 0.41 1.00 0.24. Administration EFPP, especially high dose was...

Lophatheri Herba was demonstrated to be rich in flavonoid glycosides, therefore, it is of great importance explore the high-value utilization. In this regard, these constituents own significant antioxidant capacities protect human body from aforementioned health problems. The aim article optimize and screen main antioxidative components ethanol extraction Herba. Response surface methodology (RSM) used for enhanced recovery total content (TFC), combined with two vitro methodologies measuring...

Excessive organic acids in grape juice will not only result poor taste but also cause turbidity and sedimentation. Tartaric acid exerts the most significant acidity among all juice. In this study, we used tartaric as main target anion-exchange resin to remove from concentrated Factors influencing removal process were optimized by liquid chromatography with ultraviolet detection statistical analysis for optimal deacidification of Use 335 treat at a ratio 1:6 (2:11.98) 15.57°C 4.35 hr. The...

The roots and rhizomes of Cimicifuga yunnanensis Hsiao are commonly used as anti-inflammatory, antipyretic, analgesic remedies detoxification agents in traditional Chinese medicine (TCM). Although C. has been considered supplementary for several disorders, the antitumor effect this herb its key components not explored.The were isolated by chromatographic techniques. Structures compounds identified based on spectroscopic methods comparison with published data. vitro anticancer activities...

A reversed-phase high-performance liquid chromatographic method was developed for the simultaneous quantification of seven phenolic compounds from Rhizoma Sparganii. The samples were separated on a Waters SunFire C18 column with temperature 30°C. Gradient elution applied, using 0.8% acetic acid (solvent A) and methanol B) flow rate 1.0 mL/min, detection wavelength 280 nm. validation included linearity, precision, repeatability, stability recovery. calibration curves showed good linear...

The preparation method and the sources of metal elements may affect activity metal–polysaccharide complex. In this study, four Fe–tea polysaccharide complexes were prepared three tea polysaccharides (TPSs) from different seasons extracted. Moreover, binding mode TPSs with internal external metallic as well their inhibitory effect on α-glucosidase was explored. results revealed that (–C–O–Fe –C–Fe) Fe–TPS complex at pH 5.0 closer to TPS elements. least amount (61.72 mg/g) exhibited a high...

Lophatherum gracile Brongn. (L. gracile) has been used for food and medicine in China thousands of years. Flavonoids are one the main bioactive components this herb. A reliable method was established using liquid chromatography with tandem mass spectrometry to determine concentrations seven flavonoids. The separation performed a C18 column at 40°C mobile phase mixture methanol 0.3% formic acid (v/v) water gradient elution mode flow rate 1 mL/min. Quantification through negative electrospray...

A new isocoumarin derivative 8,5'-dihydroxy-6'-methoxy-4-phenyl-5,2'-oxidoisocoumarin (1) and a stilbenoid methyl 5-hydroxy-2-(2-hydroxyphenyl)benzofuran-4-carboxylate (2) together with nine known compounds (3-11) were isolated from the tubers of Sparganium stoloniferum Buch.-Ham.. Another (3) xanthone (4) identified as natural products 5-10 obtained for first time genus Sparganium. All their structures elucidated by comprehensive spectroscopic analysis comparison available literature information.

In this study, a low‐cost efficient online derivatization system was developed which allows for the detection of various types mono‐ and oligo‐saccharides only utilizing high‐performance liquid chromatography (HPLC)‐ultraviolet detector (UV) system. proposed method, phenylhydrazine used as reagent directly spiked in mobile phase, allowing separation oligosaccharides an accessible instrument (HPLC‐UV). And design method has significantly reduced potential harm reagents to analysts....

The rhizome of Sparganium stoloniferum Buch.-Ham has been used as a traditional Chinese folk medicine for thousands years. Phenolic compounds are the main bioactive ingredients plant. In order to determine content phenolic from different major cultivations, reliable method developed using ultra-high performance liquid chromatography coupled with triple quadrupole electrospray tandem mass spectrometry. Seven compounds, including rutin, kaempferol, p-hydroxybenzaldehyde, formononetin, ferulic...

Two independent field trials were performed in Guizhou and Hunan, China 2013 to investigate the dissipation residue levels of saisentong tobacco soil. A novel accurate method using high-performance liquid chromatography with diode array detection was developed validated determine The average recovery at fortification 0.5, 2.5, 5.0 50.0 mg kg−1 fresh ranged from 75.92 107.40% a relative standard deviation (RSD) 0.94 7.55%, that 2.0 powder 74.96 94.43% 4.38 8.14%, 0.1, 0.5 soil 86.90 100.0% an...