A5100395697- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Nanoplatforms for cancer theranostics
- Immune Cell Function and Interaction
- Biosensors and Analytical Detection
- Advanced Nanomaterials in Catalysis
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- T-cell and B-cell Immunology
- Mosquito-borne diseases and control
- Nanoparticle-Based Drug Delivery
- CRISPR and Genetic Engineering
- Nanopore and Nanochannel Transport Studies
- Herpesvirus Infections and Treatments
- Viral Infections and Vectors
- Cancer Immunotherapy and Biomarkers
- RNA Research and Splicing
- Cancer, Hypoxia, and Metabolism
- Sulfur Compounds in Biology
- Cancer Genomics and Diagnostics
- Photodynamic Therapy Research Studies
- Nanocluster Synthesis and Applications
- Graphene and Nanomaterials Applications
- Cancer, Lipids, and Metabolism
- Viral Infections and Immunology Research
Chinese PLA General Hospital
2024-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2014-2025
Northeast Normal University
2022-2025
National Clinical Research Center for Digestive Diseases
2025
Wuhan University of Technology
2025
Southwest University
2016-2024
China Academy of Building Research
2024
Zhengzhou University
2023-2024
Fujian Medical University
2016-2024
Mengchao Hepatobiliary Hospital
2016-2024
Herein, a novel fluorescence sensor based on g-C3N4 nanosheet-MnO2 sandwich nanocomposite has been developed for rapid and selective sensing of glutathione (GSH) in aqueous solutions, as well living cells. The graphitic-phase C3N4 (g-C3N4) nanosheet used here is new type carbon-based nanomaterial with high quantum yield specific surface area. We demonstrate facile one-step approach the synthesis first time. this quenched, which attributing to resonance energy transfer (FRET) from deposited...
Intestinal inflammation is prevalent in chicken, which results decreased growth performance and considerable economic losses. Accumulated findings established the close relationship between gut microbiota chicken performance. However, whether impacts by lessening intestinal remains elusive.Seven-weeks-old male female chickens with highest or lowest body weights were significantly different breast leg muscle indices average cross-sectional area of cells. 16S rRNA gene sequencing indicated...
Tumors develop strategies to evade immunity by suppressing antigen presentation. In this work, we show that prosaposin (pSAP) drives CD8 T cell-mediated tumor and its hyperglycosylation in dendritic cells (DCs) leads cancer immune escape. We found lysosomal pSAP single-saposin cognates mediated disintegration of cell-derived apoptotic bodies facilitate presentation membrane-associated cell activation. the microenvironment, transforming growth factor-β (TGF-β) induced subsequent secretion,...
The budding yeast transcriptional activator Gcn4 is rapidly degraded in an SCF Cdc4 -dependent manner vivo. Upon fractionation of extracts to identify factors that mediate ubiquitination, we found Srb10 phosphorylates and thereby marks it for recognition by ubiquitin ligase. a physiological regulator stability because both phosphorylation turnover are diminished srb10 mutants. almost completely stabilized srb10Δ pho85Δ cells, or upon mutation all sites within Gcn4, suggesting the Pho85...
The human double-stranded RNA-dependent protein kinase (PKR) is an important component of the interferon response to virus infection. activation PKR accompanied by autophosphorylation at multiple sites, including one in N-terminal regulatory region (Thr-258) that required for full activity. Several kinases are activated phosphorylation between subdomains VII and VIII, referred as loop. We show Thr-446 Thr-451 loop vivo vitro high-level Mutation either residue Ala impaired translational...
Bright blue fluorescent glutathione-functionalized graphene quantum dots (GQDs@GSH) were prepared by a one-step pyrolysis method with fluorescence yield as high 33.6%. Futhermore, the obtained GQDs@GSH can be used probe to estimate ATP level in cell lysates and human blood serum.
One-pot synthesized DNA-templated Ag/Pt bimetallic nanoclusters possess highly-efficient peroxidase-like catalytic activity and are applied to design a colorimetric aptasensor for the protein detection with high sensitivity selectivity.
Cancer stem cells are considered responsible for many important aspects of tumors such as their self-renewal, tumor-initiating, drug-resistance and metastasis. However, the genetic basis origination human bladder cancer (BCSCs) remains unknown. Here, we conducted single-cell sequencing on 59 including BCSCs, non-stem (BCNSCs), epithelial (BESCs) (BENSCs) from three (BC) specimens. Specifically, BCSCs demonstrate clonal homogeneity suggest origin BESCs or BCNSCs through phylogenetic analysis....
Abstract Glucocorticoid-induced TNFR-related protein (GITR) is a costimulatory molecule with diverse effects on effector T cells and regulatory (Tregs), but the underlying mechanism remains poorly defined. Here we demonstrate that GITR ligation subverts induction of Foxp3 + Tregs directs activated CD4 to Th9 cells. Such GITR-mediated iTreg enhances anti-tumour immunity in vivo . Mechanistically, upregulates NF-κB family member p50, which recruits histone deacetylases locus produce ‘closed’...
Naive CD4+ T cells can be converted to Foxp3+ regulatory (Tregs) in the periphery (iTregs), where induction of Foxp3 gene expression is central Treg differentiation. OX40 signaling known inhibit and induction, but underlying mechanisms remain poorly defined. Here, we found that costimulation activates two distinct molecular pathways suppress freshly activated naive cells. Specifically, upregulates BATF3 BATF, which produce a closed chromatin configuration repress Sirt1/7-dependent manner....
In this work, on the basis of a new 2D DNA nanoprobe (DNP) and an enzyme-free-target-recycling amplification, electrochemical biosensor is developed for ultrasensitive detection microRNA-21 (miRNA-21). Herein, two ferrocene-labeled bipedal DNPs, which show small steric hindrance strong stability, are prepared mechanism proximity-ligation assay (PLA), improving space utilization. presence target, miRNA-21, hairpin strand, DNP will collapse, then strands miRNA-21 be simultaneously released...
Highlights•EBVaICC accounted for 6.6% of ICCs and was associated with EBV latency type I infection clonal isolates.•EBVaICChas unique characteristics related to etiology, clinicopathology molecular genetics.•Both PD-1 PD-L1 in tumor-infiltrating lymphocytes tumor cells are overexpressed EBVaICC.•Patients EBVaICC may respond well immune checkpoint inhibitors.•The lymphoepithelioma-like subtype is favorable survival.AbstractBackground & AimsLittle known about Epstein-Barr virus...
Efficient and cancer cell-targeted delivery of photosensitizer (PS) therapeutic protein has great potentiality for improving the anticancer effects. Herein, zeolitic imidazolate framework-8 (ZIF-8) nanoparticles, one most attractive metal–organic framework materials, were used coencapsulating chlorin e6 (Ce6, a potent PS) cytochrome c (Cyt c, apoptosis inducer); then nanoparticle was subsequently decorated with hyaluronic acid (HA) shell to form cell-active targeted nanoplatform (Ce6/Cyt...
Herein, a novel ratiometric electrochemical biosensor with methylene blue (MB) as the only one signal tag was proposed for highly reliable and ultrasensitive detection of microRNA-21 (miRNA-21) under assistance an intelligent target-induced dual amplification (T-DSA). First, small amount target miRNA-21 could produce abundant mimic targets DNA S1 Zn2+ through recycle acid dissolution, respectively. Then, triggered rolling circle (RCA) to generate functional nanospheres (DSP) encoded by...
Herein, by directly limiting the reaction space, an ingenious three-dimensional (3D) DNA walker (IDW) with high walking efficiency is developed for rapid and sensitive detection of miRNA. Compared traditional walker, IDW immobilized tetrahedral nanostructure (DTN) brings stronger kinetic thermodynamic favorability resulting from its improved local concentration space confinement effect, accompanied a quite faster speed much better efficiency. Once traces target miRNA-21 react prelocked IDW,...
A novel ultrasensitive electrochemiluminescence (ECL) biosensor was constructed using two-dimensional (2D) Co3O4 nanosheets as a coreaction accelerator of the luminol/H2O2 ECL system for detection microRNA-21 (miRNA-21). Impressively, 2D with effective mutual conversion Co2+/Co3+ redox pair and abundant active sites could promote decomposition coreactant H2O2 to generate more superoxide anion radicals (O2•-), which reacted luminol significantly enhancing signals. Furthermore, trace target...
Only five of the nine subunits human eukaryotic translation initiation factor 3 (eIF3) have recognizable homologs encoded in theSaccharomyces cerevisiae genome, and only two these (Prt1p Tif34p) were identified previously as yeast eIF3. We purified a polyhistidine-tagged form Prt1p (His-Prt1p) by Ni2+ affinity gel filtration chromatography obtained complex ≈600 kDa composed six polypeptides whose copurification was completely dependent on polyhistidine tag His-Prt1p. All associated with...