A5079573168- Alzheimer's disease research and treatments
- Fungal and yeast genetics research
- Microbial Metabolic Engineering and Bioproduction
- Evolution and Genetic Dynamics
- Retinal Development and Disorders
- Hepatitis C virus research
- Neurological Disease Mechanisms and Treatments
- Dementia and Cognitive Impairment Research
- Hepatitis B Virus Studies
- Glaucoma and retinal disorders
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Advanced Chemical Sensor Technologies
- Receptor Mechanisms and Signaling
- Genomics and Phylogenetic Studies
- DNA Repair Mechanisms
- Parkinson's Disease Mechanisms and Treatments
- Prion Diseases and Protein Misfolding
- Cancer Genomics and Diagnostics
- Photosynthetic Processes and Mechanisms
- Photoreceptor and optogenetics research
- Retinal Imaging and Analysis
- RNA regulation and disease
- Retinal Diseases and Treatments
- Neurological diseases and metabolism
Xiangya Hospital Central South University
2020-2025
Central South University
2020-2025
Stanford University
1999-2024
Regeneron (United States)
1993-2024
Shandong University
2019-2023
State Key Laboratory of Microbial Technology
2020-2023
Beijing Proteome Research Center
2022
Anhui Medical University
2022
The University of Queensland
2021
Wenzhou City People's Hospital
2016-2021
Mutation is the ultimate source of genetic variation. The most direct and unbiased method studying spontaneous mutations via mutation accumulation (MA) lines. Until recently, MA experiments were limited by cost sequencing thus provided us with small numbers mutational events therefore imprecise estimates rates patterns mutation. We used whole-genome to identify nearly 1,000 accumulated over ∼311,000 generations in 145 diploid lines budding yeast Saccharomyces cerevisiae. are usually assumed...
Using a genomics-based reverse pharmacological approach for screening orphan G-protein coupled receptors, we have identified and cloned novel high-affinity histamine receptor. This receptor, termed AXOR35, is most closely related to the H3 sharing 37% protein sequence identity. A multiple responsive element/cyclic AMP-responsive element-luciferase reporter assay was used identify as ligand AXOR35. When transfected into human embryonic kidney 293 cells, AXOR35 receptor showed strong,...
Leukemia inhibitory factor (LIF) is a cytokine that affects the survival and differentiation of certain neuronal populations in vitro. To identify LIF-responsive neurons adult rat, we have demonstrated retrograde axonal transport 125I-LIF to sensory motor neurons. The accumulation by both cell types was significantly increased prior sciatic nerve crush. Retrograde inhibited excess unlabeled LIF but not related cytokines, indicating specific receptor-mediated mechanism. Northern blot analysis...
Budding yeast has undergone several independent transitions from commercial to clinical lifestyles. The frequency of such suggests that strains are derived environmentally available populations, including sources. However, despite their important role in adaptive evolution, the prevalence polyploidy and aneuploidy not been extensively analyzed strains. In this study, we have looked for patterns governing transition invasion largest screen isolates date. particular, focused on hypothesis...
The protein-folding chaperone Hsp90 has been proposed to buffer the phenotypic effects of mutations. potential for and other putative buffers increase robustness mutation had major impact on disease models, quantitative genetics, evolutionary theory. But sometimes contradicts expectations a by potentiating rapid changes that would otherwise not occur. Here, we quantify Hsp90's ability or potentiate (i.e., diminish enhance) genetic variation single-cell morphological features in budding...
Microscopic analysis of urine sediment is probably the most commonly used diagnostic procedure in nephrology. The urinary cells, however, have not yet undergone careful unbiased characterization.
The cognitive impairment patterns and the association with Alzheimer's disease (AD) in mental disorders remain poorly understood.
The sequencing of pooled non-barcoded individuals is an inexpensive and efficient means assessing genome-wide population allele frequencies, yet its accuracy has not been thoroughly tested. We assessed the this approach on whole, complex eukaryotic genomes by resequencing pools largely isogenic, individually sequenced Drosophila melanogaster strains. called SNPs in data estimated false positive negative rates using individual strain as a reference. also frequency "pooled" compared them with...
Associations between chronic antigen stimulation, T cell dysfunction, and the expression of various inhibitory receptors are well characterized in several mouse human systems. During hepatitis B virus (HBV) infection (CHB), responses blunted with low frequencies virus-specific cells observed, making these parameters difficult to study. Here, using mass cytometry a highly multiplexed combinatorial peptide-major histocompatibility complex (pMHC) tetramer strategy that allows for detection rare...
CRISPR/Cas9-assisted recombineering is restricted in lactic acid bacteria because of the lack available antibiotics and vectors. In this study, a seamless genome editing method was carried out Lactobacillus plantarum using double-stranded DNA (dsDNA) single-stranded (ssDNA) recombineering, recombination efficiency effectively improved by endogenous adenine-specific methyltransferase overexpression. L. WCFS1 produced 797.3 mg/liter N -acetylglucosamine (GlcNAc) through reinforcement GlcNAc...
Abstract Aims We mainly evaluate retinal alterations in Alzheimer's disease (AD) patients, investigate the associations between changes with AD biomarkers, and explore an optimal machine learning (ML) model for diagnosis based on thickness. Methods A total of 159 patients 299 healthy controls were enrolled. The parameters each participant measured using optical coherence tomography (OCT). Additionally, cognitive impairment severity, brain atrophy, cerebrospinal fluid (CSF) biomarkers...
Abstract Introduction We explored whether volatile organic compound (VOC) detection can serve as a screening tool to distinguish cognitive dysfunction (CD) from cognitively normal (CN) individuals. Methods The function of 1467 participants was assessed and their VOCs were detected. Six machine learning algorithms conducted the performance determined. plasma neurofilament light chain (NfL) measured. Results Distinguished VOC patterns existed between CD CN groups. model showed good accuracy...
Abstract INTRODUCTION Whether the integration of eye‐tracking, gait, and corresponding dual‐task analysis can distinguish cognitive impairment (CI) patients from controls remains unclear. METHODS One thousand four hundred eighty‐one participants, including 724 CI 757 controls, were enrolled in this study. Eye movement combined with patterns, measured. The LightGBM machine learning models constructed. RESULTS A total 105 gait eye‐tracking features extracted. Forty‐six parameters, 32 14...
Whether volatile organic compounds (VOCs) from exhaled breath can be used as a novel biomarker for Alzheimer's disease (AD) diagnosis is unclear. To determine the significantly distinctive VOCs AD, total of 970 participants were enrolled, including 60 individuals in data set 1 (AD, 30; controls, 30), 164 2 82; 82), 637 3 31; 606), and 109 4 (frontotemporal dementia, 19; vascular 21; Parkinson's disease, 69). The sets 1, 2, Xiangya Hospital, Central South University. Participants two-year...
Current challenge for dynamic pathway control in metabolic engineering is enabling the components of artificial regulatory system to be tunable. Here, we designed and built a heme-responsive containing heme biosensor HrtR CRISPRi regulate chemicals production while maintaining intracellular homeostasis. A series engineered biosensors with varied sensitivity threshold were obtained by semi-rational design site saturated mutation HrtR. The modified metabolite-binding affinity was confirmed...