A5080148797- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Structure and Function
- Microbial Community Ecology and Physiology
- CRISPR and Genetic Engineering
- 3D Printing in Biomedical Research
- Pharmaceutical and Antibiotic Environmental Impacts
- Gene Regulatory Network Analysis
- Viral gastroenteritis research and epidemiology
- Cytomegalovirus and herpesvirus research
- Enzyme Catalysis and Immobilization
- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Glycosylation and Glycoproteins Research
- bioluminescence and chemiluminescence research
- Chemical Synthesis and Analysis
- Biochemical and Molecular Research
- Enzyme Production and Characterization
State Key Laboratory of Microbial Technology
2017-2022
Shandong University
2017-2022
CRISPR/Cas9-assisted recombineering is restricted in lactic acid bacteria because of the lack available antibiotics and vectors. In this study, a seamless genome editing method was carried out Lactobacillus plantarum using double-stranded DNA (dsDNA) single-stranded (ssDNA) recombineering, recombination efficiency effectively improved by endogenous adenine-specific methyltransferase overexpression. L. WCFS1 produced 797.3 mg/liter N -acetylglucosamine (GlcNAc) through reinforcement GlcNAc...
N-acetyl-d-neuraminic acid (NeuAc) has attracted considerable attention because of its wide-ranging applications. The use cheap carbon sources such as glucose without the addition any precursor in microbial NeuAc production many advantages. In this study, improved was attained through optimization amino sugar metabolism pathway kinetics and reservation a phosphoenolpyruvate (PEP) pool Escherichia coli. N-acylglucosamine 2-epimerase N-acetylneuraminate synthase from different their best...
The assembly of DNA fragments is extremely important for molecular biology. Increasing numbers studies have focused on streamlining the laborious and costly protocols via in vivo assembly. However, existing methods were mainly developed Escherichia coli or Saccharomyces cerevisiae, whereas there are few direct other microorganisms. use shuttle vectors tedious plasmid extraction transformation procedures make cloning microorganisms inefficient, especially library construction. In this study,...
Traditional methods of microbial synthesis usually rely on a single engineered strain to synthesize the target product through metabolic engineering. The key cofactors, precursors and energy are produced by introduced complex synthetic pathways. This would increase physiological burden engineering strains, resulting in decrease yield products. modular co-culture has become an attractive solution for effective heterologous biosynthesis, where can be greatly improved. In engineering,...