A5100744739- Tea Polyphenols and Effects
- Machine Learning in Bioinformatics
- Plant Stress Responses and Tolerance
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Tuberculosis Research and Epidemiology
- Plant nutrient uptake and metabolism
- Mycobacterium research and diagnosis
- Plant Molecular Biology Research
- Fractal and DNA sequence analysis
- GABA and Rice Research
- Protein Structure and Dynamics
- Plant Pathogens and Fungal Diseases
- Genomics and Phylogenetic Studies
- Photosynthetic Processes and Mechanisms
- Phytochemicals and Antioxidant Activities
- Infectious Diseases and Tuberculosis
- COVID-19 Impact on Reproduction
- Gut microbiota and health
- CRISPR and Genetic Engineering
- Food composition and properties
- Plant Gene Expression Analysis
- COVID-19 Clinical Research Studies
- Selenium in Biological Systems
- Inflammatory Bowel Disease
Lanzhou University
2025
Anhui Medical University
2025
Shenyang Agricultural University
2025
Chinese Academy of Agricultural Sciences
2016-2024
Tea Research Institute
2012-2024
Ministry of Agriculture and Rural Affairs
2018-2024
Second Affiliated Hospital of Harbin Medical University
2022-2024
Agricultural Genomics Institute at Shenzhen
2021-2024
Shandong Academy of Agricultural Sciences
2022-2024
Harbin Medical University
2022-2024
Abstract Complete and accurate reference genomes annotations provide fundamental resources for functional genomics crop breeding. Here we report a de novo assembly annotation of pea cultivar ZW6 with contig N50 8.98 Mb, which features 243-fold increase in length evident improvements the continuity quality sequence complex repeat regions compared existing one. Genome diversity 118 cultivated wild demonstrated that Pisum abyssinicum is separate species different from P. fulvum sativum within ....
Tea plant (Camellia sinensis) is one of the most economically valuable crops in world. Anthracnose can affect growth leaves and cause serious yield losses tea. plants are rich secondary metabolites; however, their roles resistance to anthracnose unclear. Herein we compared contents total phenolics, catechins, caffeine two cultivars with different resistances during Colletotrichum fructicola infection. (-)-Epigallocatechin-3-gallate (EGCG), (+)-catechin (C), caffeine, critical regulatory...
Basic region/leucine zipper (bZIP) transcription factors play vital roles in the abiotic stress response of plants. However, little is known about function bZIP genes Camellia sinensis .CsbZIP6 was overexpressed Arabidopsis thaliana . Effects CsbZIP6 overexpression on abscisic acid (ABA) sensitivity, freezing tolerance and expression cold-responsive arabidopsis were studied.CsbZIP6 induced during cold acclimation tea plant. Constitutive lowered plants' to ABA exposure seedling growth....
Anthracnose causes severe losses of tea production in China. Although genes and biological processes involved anthracnose resistance have been reported other plants, the molecular response to plant is unknown. We used susceptible cultivar Longjing 43 resistant Zhongcha 108 as materials compared transcriptome changes leaves both cultivars following Colletotrichum fructicola inoculation. In all, 9015 8624 were differentially expressed between their controls (0 h), respectively. cultivars,...
Abstract Efficient and precise base editors (BEs) for C-to-G transversion are highly desirable. However, the sequence context affecting editing outcome largely remains unclear. Here we report engineered BEs of high efficiency fidelity, with predictable via machine-learning methods. By changing species origin relative position uracil-DNA glycosylase deaminase, together codon optimization, obtain optimized (OPTI-CGBEs) efficient transversion. The motif preference OPTI-CGBEs 100 endogenous...
Three commercial pea proteins and a laboratory sample (PL) were assessed for their physicochemical properties (subunit composition, secondary structure, surface hydrophobicity, solubility) gelation (rheology, texture, intermolecular forces) in fermentation induced yogurt gel. The results showed that the dissociation of legumin was occurred all three proteins, accompanied by formation large protein aggregates. contents α-helix β-sheet significantly lower than PL (p < 0.05). However, prepared...
Background Alterations in lipid metabolism promote tumor progression. However, the role of occurrence and development gastric cancer have not been fully clarified Method Here, genes that are related to fatty acid differentially-expressed between normal tissues were identified TCGA-STAD cohort. The intersection with Geneset was determined obtain 78 metabolism-related genes. ConsensusClusterPlus R package used perform genes, which yielded divided two subtypes termed cluster 1 2. Results...
Abstract Base editors have been reported to induce off-target mutations in cultured cells, mouse embryos and rice, but their long-term effects vivo remain unknown. Here, we develop a Systematic evaluation Approach For gene Editing tools by Transgenic mIce (SAFETI), evaluate the of BE3, high fidelity version CBE (YE1-BE3-FNLS) ABE (ABE7.10 F148A ) ~400 transgenic mice over 15 months. Whole-genome sequence analysis reveals BE3 expression generated de novo offspring mice. RNA-seq both...
Abstract Adenine base editors (ABEs) and cytosine (CBEs) enable the single nucleotide editing of targeted DNA sites avoiding generation double strand breaks, however, genomic features that influence outcomes in vivo still remain to be characterized. High-throughput datasets from lentiviral integrated libraries were used investigate sequence affecting outcomes, but effects endogenous factors beyond sequences are largely unknown. Here ABE CBE evaluated mammalian cells for 5012 11,868...
Abstract Sialylation is one of the altered glycosylation patterns associated with cancer progression. In this study, we investigated N -glycan profiles breast patients and cell lines to reveal sialylation progression, provided new evidences miRNA-mediated sialylation. MALDI-TOF MS analysis revealed that -glycans found in tissues MDA-MB-231 featured increased levels compared adjacent normal epithelial MCF-10A. The expressional 20 sialyltransferase genes were then analyzed significantly...
Colletotrichum, the causative agent of anthracnose, is an important pathogen that invades tea plant (Camellia sinensis). In this study, 38 isolates were obtained from diseased leaves plants collected in different areas Zhejiang Province, China. A combination multigene (ITS, ACT, GAPDH, TUB2, CAL, and GS) morphology analyses showed strains belonged to two species, namely, C. camelliae (CC), fructicola (CF). Pathogenicity tests revealed CC was more invasive than CF. vitro inoculation...
Vacuolar invertases (VINs) have been reported to regulate plant growth and development respond abiotic stresses such as drought cold. With our best knowledge, the functions of VIN genes little in tea (Camellia sinensis L.). Therefore, it is necessary develop research this field. Here, we identified a gene, CsINV5, which was induced by cold acclimation sugar treatments plant. Histochemical assays results showed that 1154 bp 5′-flanking sequence CsINV5 drove β-glucuronidase (GUS) gene...
Primary healthcare (PHC) plays a key role in hyperlipidemia (HL) management yet lacks adequate monitoring and feedback. This study aims at identifying pragmatic measures out from routinely collected electronic records to enable automatic inform continuous optimization of HL-management PHC settings. The used randomly selected (from the province-wide data center Anhui-province, China) as main source generated both procedure-based encounter-based for assessing HL-management. were derived...
To explore the relationship between serum complement levels at diagnosis and prognosis in patients with aggressive non-Hodgkin lymphoma(NHL). The clinical data of 102 lymphoma diagnosed First Hospital Lanzhou University from February 2017 to March 2023 were selected analyze correlation C3 C4 NHL time initial diagnosis. optimal cut-off point obtained by calculating Jorden index through receiver operating characteristic（ROC） curve, divided into low group (C3< 1.07) high (C3≥1.07), (C4< 0.255)...
Bacteriocytes are specialized insect cells adapted to harbor symbionts. However, their low number in individual whiteflies makes obtaining enough for transcriptome sequencing challenging. Here, we present a protocol the isolation of whitefly bacteriocytes. We describe steps preparing single bacteriocyte sample, cDNA amplification library construction, sequencing, and data analysis. Our offers convenience efficiency conducting single-cell bacteriocytes has potential be applied other cells....