A5080216043- Cell Image Analysis Techniques
- Image Processing Techniques and Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Chemokine receptors and signaling
- Photoacoustic and Ultrasonic Imaging
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Neuroinflammation and Neurodegeneration Mechanisms
- 3D Printing in Biomedical Research
- Tryptophan and brain disorders
- Immune Response and Inflammation
- Advanced Fluorescence Microscopy Techniques
- HER2/EGFR in Cancer Research
- Cell Adhesion Molecules Research
- interferon and immune responses
- Melanoma and MAPK Pathways
- Labor market dynamics and wage inequality
- Immune Cell Function and Interaction
- Advanced Glycation End Products research
- vaccines and immunoinformatics approaches
- Ubiquitin and proteasome pathways
Weizmann Institute of Science
2024-2025
Università della Svizzera italiana
2020-2024
Institute of Cell Biology
2024
University of Bern
2020-2024
Sapienza University of Rome
1977
For decades, antigen presentation on major histocompatibility complex class I for T cell-mediated immunity has been considered the primary function of proteasome-derived peptides1,2. However, whether products proteasomal degradation play additional parts in mounting immune responses remains unknown. Antimicrobial peptides serve as a first line defence against invading pathogens before adaptive system responds. Although protective antimicrobial across numerous tissues is well established,...
Chemotaxis is an essential physiological process, often harnessed by tumors for metastasis. CXCR4, its ligand CXCL12 and the atypical receptor ACKR3 are overexpressed in many human cancers. Interfering with this axis deletion impairs lymphoma cell migration towards CXCL12. Here, we propose a model of how controls diffused large B-cell VAL cells vitro vivo response to expressing full-length ACKR3, but not truncated version missing C-terminus, can support lacking (VAL-ko) when allowed migrate...
Immune responses highly depend on the effective trafficking of immune cells into and within secondary lymphoid organs (SLOs). Atypical chemokine receptors (ACKRs) scavenge chemokines to eliminate them from extracellular space, thereby generating gradients that guide leukocytes. In contrast canonical receptors, ACKRs do not induce classical intracellular signaling results in cell migration. Recently, closest relative ACKR3, GPR182, has been partially deorphanized as a potential novel ACKR. We...
The marginal zone (MZ) contributes to the highly organized spleen microarchitecture. We show that expression of atypical chemokine receptor 3 (ACKR3) defines two equal-sized populations mouse MZ B cells (MZBs). ACKR3 is required for development a functional and positioning MZBs. Deletion on distorts MZ, MZBs fail deliver antigens follicles, reducing humoral responses. Reconstitution MZ-deficient CD19ko mice shows ACKR3− can differentiate into ACKR3+ MZBs, but not vice versa. lack rescued by...
Intravital microscopy has revolutionized live-cell imaging by allowing the study of spatial–temporal cell dynamics in living animals. However, complexity data generated this technology limited development effective computational tools to identify and quantify processes. Amongst them, apoptosis is a crucial form regulated death involved tissue homeostasis host defense. Live-cell enabled at cellular level, enhancing our understanding its regulation. present, no method can deliver robust...
Abstract Cell migration is a pivotal biological process, whose dysregulation found in many diseases including inflammation and cancer. Advances microscopy technologies allow now to study cell vitro, within engineered microenvironments that resemble vivo conditions. However, capture an entire 3D chamber for extended periods of time with high temporal resolution, images are generally acquired low which poses challenge data analysis. Indeed, detection tracking hampered due the large pixel size...
Intravital microscopy has revolutionized live-cell imaging by allowing the study of spatial–temporal cell dynamics in living animals. However, complexity data generated this technology limited development effective computational tools to identify and quantify processes. Amongst them, apoptosis is a crucial form regulated death involved tissue homeostasis host defense. Live-cell enabled at cellular level, enhancing our understanding its regulation. present, no method can deliver robust...
Intravital microscopy has revolutionized live cell imaging by allowing the study of spatial-temporal dynamics in living animals. However, complexity data generated this technology limited development effective computational tools to identify and quantify processes. Amongst them, apoptosis is a crucial form regulated death involved tissue homeostasis host defense. Live-cell enabled at cellular level, enhancing our understanding its regulation. present, no method can deliver robust detection...
Abstract Intravital microscopy has revolutionized live cell imaging by allowing the study of spatial-temporal dynamics in living animals. However, complexity data generated this technology limited development effective computational tools to identify and quantify processes. Amongst them, apoptosis is a crucial form regulated death involved tissue homeostasis host defense. Live-cell enabled at cellular level, enhancing our understanding its regulation. present, no method can deliver robust...
Abstract Cell migration is a pivotal biological process, whose dysregulation found in many diseases including inflammation and cancer. Advances microscopy technologies allow now to study cell vitro , within microenvironments that resemble vivo conditions. However, when cells are observed large 3D chambers at low magnification for extended periods of time, data analysis becomes difficult. Indeed, detection tracking hampered due the pixel size, possible signal-to-noise ratio distortions shape...
Intravital microscopy has revolutionized live cell imaging by allowing the study of spatial-temporal dynamics in living animals. However, complexity data generated this technology limited development effective computational tools to identify and quantify processes. Amongst them, apoptosis is a crucial form regulated death involved tissue homeostasis host defense. Live-cell enabled at cellular level, enhancing our understanding its regulation. present, no method can deliver label-free...