A5011111664- Prion Diseases and Protein Misfolding
- Cancer Immunotherapy and Biomarkers
- Immune cells in cancer
- Immunotherapy and Immune Responses
- IL-33, ST2, and ILC Pathways
- Monoclonal and Polyclonal Antibodies Research
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Trace Elements in Health
- Influenza Virus Research Studies
- Adenosine and Purinergic Signaling
- RNA regulation and disease
- Alzheimer's disease research and treatments
- Toxin Mechanisms and Immunotoxins
- Biochemical and Molecular Research
- Transgenic Plants and Applications
- RNA Interference and Gene Delivery
- Pregnancy and Medication Impact
- Respiratory viral infections research
- HIV Research and Treatment
- Extracellular vesicles in disease
- Reproductive System and Pregnancy
- Advanced Biosensing Techniques and Applications
- Pediatric Pain Management Techniques
- Neurological diseases and metabolism
Università della Svizzera italiana
2018-2024
University of Bern
2018-2022
Fondazione IRCCS Istituto Neurologico Carlo Besta
2015-2019
Istituti di Ricovero e Cura a Carattere Scientifico
2015
Significance Here we report that targeting the pattern recognition receptor MARCO on macrophages within tumor alters their polarization and in turn activate natural killer (NK) cells to kill tumor. We describe mechanism of action, including NK are induced use receptor-mediated killing cancer cells. Furthermore, find this type treatment works combination with T cell-targeted checkpoint therapies. then transfer finding humans similar subpopulations human also block Finally, these a new...
Despite safety concerns regarding the toxicity of tattoo ink, no studies have reported consequences tattooing on immune response. In this work, we characterized transport and accumulation different inks in lymphatic system using a murine model. Upon quick drainage, observed that macrophages mainly capture ink lymph node (LN). An initial inflammatory reaction at local systemic levels follows capture. Notably, process is maintained over time as clear signs inflammation draining LN two months...
Abstract We developed a novel therapeutic strategy for Alzheimer’s disease (AD) exploiting the properties of natural variant Amyloid-β (Aβ) carrying A2V substitution, which protects heterozygous carriers from AD by its ability to interact with wild-type Aβ, hindering conformational changes and assembly thereof. As prototypic compound we designed six-mer mutated peptide (Aβ1-6 ), linked HIV-related TAT protein, is widely used brain delivery cell membrane penetration drugs. The resulting...
Prions are infectious proteins that possess multiple self-propagating structures. The information for strains and structural specific barriers appears to be contained exclusively in the folding of pathological isoform, PrPSc. Many recent studies determined de novo prion could generated vitro from conversion recombinant (rec) protein (PrP) into amyloidal Our aim was elucidate conformational diversity recPrP amyloids their biological activities, as well gain novel insights characterizing...
Neutrophils are amongst the first cells to respond inflammation and infection. Although they play a key role in limiting dissemination of pathogens, study their dynamic behavior immune organs remains elusive. In this work, we characterized vivo neutrophils mouse popliteal lymph node (PLN) after influenza vaccination with UV-inactivated virus. To achieve this, used an image-based systems biology approach detect motility patterns associate them distinct actions. We described prominent rapid...
mRNA-based vaccines symbolize a new paradigm shift in personalized medicine for the treatment of infectious and non-infectious diseases. However, reactogenicity associated with currently approved formulations limits their applicability autoinflammatory disorders, such as tumour therapeutics. In this study, we present delivery system showing controlled immunogenicity minimal non-specific inflammation, allowing selective mRNA to antigen presenting cells (APCs) within medullary region lymph...
Antibodies to the prion protein, PrP, represent a promising therapeutic approach against diseases but neurotoxicity of certain anti-PrP antibodies has caused concern. Here we describe scPOM-bi, bispecific antibody designed function as molecular tweezer. scPOM-bi combines complementarity-determining regions neurotoxic POM1 and neuroprotective POM2, which bind globular domain (GD) flexible tail (FT) respectively. We found that confers protection prion-infected organotypic cerebellar slices...
Abstract During melanoma metastasis, tumor cells originating in the skin migrate via lymphatic vessels to sentinel lymph node (sLN). This process facilitates cell spread across body. Here, we characterized innate inflammatory response metastatic microenvironment of sLN. We found that macrophages located subcapsular sinus (SS) produced protumoral IL1α after recognition tumoral antigens. Moreover, confirmed elimination LN or administration an IL1α-specific blocking antibody reduced spread. To...
Aim: To design lympho-targeted nanocarriers with the capacity to enhance activity of associated drugs/antigens whose target is within lymphatic system. Materials & methods: Inulin (INU)-based nanocapsules (NCs), negatively charged and positively chitosan NCs were prepared by solvent displacement techniques. The produced in two sizes: small (70 nm) medium (170-250 nm). Results:In vitro results indicated that interacted more efficiently dendritic cells than larger ones. study biodistribution...
The analysis of cell-cell or cell-pathogen interaction in vivo is an important tool to understand the dynamics immune response infection. Two-photon intravital microscopy (2P-IVM) allows observation cell interactions deep tissue living animals, while minimizing photobleaching generated during image acquisition. To date, different models for 2P-IVM lymphoid and non-lymphoid organs have been described. However, imaging respiratory remains a challenge due movement associated with breathing...
Prion diseases are fatal neurodegenerative disorders whose pathogenesis mechanisms not fully understood. In this context, we conducted a microarray-based transcriptome analysis of brains from BSE-infected macaques and five-gene signature was identified: in brief, HBB HBA2 were down-regulated infected macaques, whereas TTR, APOC1 SERPINA3 up-regulated. The purpose the current work is to validate CJD brain samples, where aim confirm results also at protein level. We collected about 100 samples...
The analysis of cell-cell or cell-pathogen interaction in vivo is an important tool to understand the dynamics immune response infection.Two-photon intravital microscopy (2P-IVM) allows observation cell interactions deep tissue living animals, while minimizing photobleaching generated during image acquisition.To date, different models for 2P-IVM lymphoid and non-lymphoid organs have been described.However, imaging respiratory remains a challenge due movement associated with breathing cycle...
Rectal prolapse is a common condition in laboratory-housed macaques, usually associated with recurrent gastroenteritis and stress. However, evidence for both statements lacking. Therefore, the prevalence of rectal prolapses their risk factors macaques have yet to be assessed. In addition, standard care pharmacological treatment remains recognized documented. Thus, retrospective study involving 816 was conducted, which prevalence, factors, success The shown 3.92%, all cases could either...
Pin1 is a peptidyl-prolyl isomerase that induces the cis-trans conversion of specific Ser/Thr-Pro peptide bonds in phosphorylated proteins, leading to conformational changes through which regulates protein stability and activity. Since down-regulation has been described several neurodegenerative disorders, including Alzheimer's Disease (AD), Parkinson's (PD) Huntington's (HD), we investigated its potential role prion diseases. Animals generated on wild-type (Pin1+/+), hemizygous (Pin1+/−) or...
<div>Abstract<p>During melanoma metastasis, tumor cells originating in the skin migrate via lymphatic vessels to sentinel lymph node (sLN). This process facilitates cell spread across body. Here, we characterized innate inflammatory response metastatic microenvironment of sLN. We found that macrophages located subcapsular sinus (SS) produced protumoral IL1α after recognition tumoral antigens. Moreover, confirmed elimination LN or administration an IL1α-specific blocking antibody...
Supplementary Figure from Subcapsular Sinus Macrophages Promote Melanoma Metastasis to the Sentinel Lymph Nodes via an IL1α–STAT3 Axis
Supplementary Data from Subcapsular Sinus Macrophages Promote Melanoma Metastasis to the Sentinel Lymph Nodes via an IL1α–STAT3 Axis
Supplementary Figure from Subcapsular Sinus Macrophages Promote Melanoma Metastasis to the Sentinel Lymph Nodes via an IL1α–STAT3 Axis
Supplementary Data from Subcapsular Sinus Macrophages Promote Melanoma Metastasis to the Sentinel Lymph Nodes via an IL1α–STAT3 Axis