A5038142685- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- IL-33, ST2, and ILC Pathways
- Immune cells in cancer
- Adenosine and Purinergic Signaling
- Immune Cell Function and Interaction
- Single-cell and spatial transcriptomics
- Cell Image Analysis Techniques
Università della Svizzera italiana
2021-2022
Abstract During melanoma metastasis, tumor cells originating in the skin migrate via lymphatic vessels to sentinel lymph node (sLN). This process facilitates cell spread across body. Here, we characterized innate inflammatory response metastatic microenvironment of sLN. We found that macrophages located subcapsular sinus (SS) produced protumoral IL1α after recognition tumoral antigens. Moreover, confirmed elimination LN or administration an IL1α-specific blocking antibody reduced spread. To...
<div>Abstract<p>During melanoma metastasis, tumor cells originating in the skin migrate via lymphatic vessels to sentinel lymph node (sLN). This process facilitates cell spread across body. Here, we characterized innate inflammatory response metastatic microenvironment of sLN. We found that macrophages located subcapsular sinus (SS) produced protumoral IL1α after recognition tumoral antigens. Moreover, confirmed elimination LN or administration an IL1α-specific blocking antibody...
Two-photon intravital microscopy (2P-IVM) has become a widely used technique to study cell-to-cell interactions in living organisms. Four-dimensional imaging data obtained via 2P-IVM are classically analyzed by performing automated cell tracking, procedure that computes the trajectories followed each cell. However, technical artifacts, such as brightness shifts, presence of autofluorescent objects, and channel crosstalking, affect specificity channels for cells interest, thus hampering...
<div>Abstract<p>During melanoma metastasis, tumor cells originating in the skin migrate via lymphatic vessels to sentinel lymph node (sLN). This process facilitates cell spread across body. Here, we characterized innate inflammatory response metastatic microenvironment of sLN. We found that macrophages located subcapsular sinus (SS) produced protumoral IL1α after recognition tumoral antigens. Moreover, confirmed elimination LN or administration an IL1α-specific blocking antibody...
Supplementary Figure from Subcapsular Sinus Macrophages Promote Melanoma Metastasis to the Sentinel Lymph Nodes via an IL1α–STAT3 Axis
Supplementary Data from Subcapsular Sinus Macrophages Promote Melanoma Metastasis to the Sentinel Lymph Nodes via an IL1α–STAT3 Axis
Supplementary Figure from Subcapsular Sinus Macrophages Promote Melanoma Metastasis to the Sentinel Lymph Nodes via an IL1α–STAT3 Axis
Supplementary Data from Subcapsular Sinus Macrophages Promote Melanoma Metastasis to the Sentinel Lymph Nodes via an IL1α–STAT3 Axis
ABSTRACT During melanoma metastasization, tumor cells originated in the skin migrate via lymphatic vessels to sentinel lymph node (sLN) a process that facilitates their spread across body. Here, we characterized innate inflammatory responses metastasis sLN. For this purpose, confirmed migration of fluorescent metastatic sLN and response microenvironment. We found macrophages located subcapsular sinus (SSM), produce pro-tumoral IL-1α after recognition antigens. Moreover, administration an...